Effect of species and spacing of fast-growing nurse trees on growth of an indigenous tree, Hopea odorata Roxb., in northeast Thailand

We tested the effects of species and spacing of nurse trees on the growth of Hopea odorata, a dipterocarp tree indigenous to Southeast Asia, in a two-storied forest management system in northeast Thailand. Eucalyptus camaldulensis, Acacia auriculiformis, and Senna siamea were planted as nurse trees in 1987 at spacings of 4 m × 8 m, 2 m × 8 m, 4 m × 4 m, and 2 m × 4 m in the Sakaerat Silvicultural Research Station of the Royal Forest Department, Thailand. Seedlings of H. odorata were planted in the nurse tree stands at a uniform spacing of 4 m × 4 m and in control plots (no nurse trees) in 1990. Stem numbers of some nurse trees were thinned by half in 1994. The stem diameter and height of all trees were measured annually until 1995 and again in 2007. The mean annual increment (MAI) in volume was estimated as 8.2–10.1 m³ ha⁻¹ year⁻¹ for E. camaldulensis and 0.9–1.2 m³ ha⁻¹ year⁻¹ for S. siamea, smaller than reported elsewhere. This suggests that the site properties were not suitable for them. The MAI of A. auriculiformis was 7.9–9.8 m³ ha⁻¹ year⁻¹, within the reported range. Survival rates of H. odorata in the S. siamea stands and the control plots decreased rapidly during the first 2 years but then stayed constant from 1992. In contrast, survival rates of H. odorata in the E. camaldulensis and A. auriculiformis stands were initially high (>70%), but then decreased after 1995. Stem diameter, tree height, and stand basal area of H. odorata were large in both the S. siamea stands and the control plots from then. The growth of H. odorata was largest in the 2 m × 8 m S. siamea stands. In contrast, it was restricted in the E. camaldulensis and A. auriculiformis stands owing to strong shading by their canopies. Thinning by 50% tended to facilitate the growth of H. odorata temporarily in the E. camaldulensis and A. auriculiformis stands. The stand basal areas of nurse trees and of H. odorata showed a trade-off. These results suggest that the growth of H. odorata was maximized in the S. siamea stands. We assume, however, that the growth of H. odorata could be improved even in the E. camaldulensis and A. auriculiformis stands by frequent or heavy thinning.     

Microvasculature of the retina, ciliary processes and choroid in the North American raccoon (Procyon lotor) eye: a scanning electron microscopic study of corrosion casts

We have studied the vasculature of the retina, ciliary processes and choroid in the North American raccoon (Procyon lotor), a nocturnal mammal, using light and scanning electron microscopic examination of corrosion casts. We carried out an identical study in the crab-eating macaque (Macaca fascicularis), which forages only during the daytime, in order to compare the ocular vasculature with that of nocturnal mammals. Our observations in raccoons demonstrated a photoreceptor layer associated with rich lymph and a poorly vascularized retina. The meridian region of the eye, which lies in the horizontal plane and pass around the optic disc, had a markedly sparse capillary network. This horizontal sparse vascular band may correspond to a visual streak. Ciliary process capillaries were delicate, and formed a well-developed and compact network. Choriocapillaries were quite thin and formed a coarse capillary network. This contrasted with the dense retinal and well-extended choroidal capillary networks noted in the macaques. Our findings suggest that the sparse retinal capillary network in raccoons is extremely beneficial for photon capture, thereby allowing the raccoon to see well at night, as the retinal vessels restrict the inflow of photons toward the photoreceptors. The well-developed lymph probably compensates for the sparse retinal capillaries and choriocapillaries and nourishes the retina in the nocturnal raccoon.     

Aquaporin 1 expression in tissues of canines possessing inherited high K+ erythrocytes

We investigated the expression of aquaporin 1 (AQP1) in tissues from canines with an inherited anomaly that causes their erythrocytes to have high K⁺. Northern blot analysis revealed abundant AQP1 expression in lung and kidney, though little expression was found in spleen. Using anti-C-terminus for dog AQP1, abundant expression was shown in kidney, trachea, and eye, but little expression was shown in pancreas and cerebrum, indicating that AQP1 expression in canine tissues is similar to that noted in other mammals.     

Mitigating the anti-nutritional effect of polyphenols on in vitro digestibility and fermentation characteristics of browse species in north western Ethiopia

Tropical Animal Health and Production - Browse species are important sources of forage for livestock in Ethiopia, especially during the dry season, when the quality and quantity of green herbage is...     

Seasonal variation of paulownia witches’-broom phytoplasma in paulownia trees and distribution of the disease in the Tohoku district of Japan

Seasonal variation of paulownia witches’-broom (PWB) phytoplasma within different organs (leaves, branch and trunk bark and roots) in paulownia trees was investigated by the amplification of a PWB-specific DNA fragment by the polymerase chain reaction (PCR). In leaf samples, PWB phytoplasma was first detected in June and the incidence gradually increased. On the other hand, the PWB was detected at relatively low incidence in branch bark, trunk bark and roots and the incidence did not change among seasons. A survey of PWB in 27 fields in the Tohoku district of Japan showed that malformed flower buds were observed in paulownia trees in almost all of the fields. PWB-phytoplasma was also detected by PCR from paulownia trees in almost all of the fields in Iwate and Fukushima Prefectures. The frequencies of trees in which phytoplasma was detected by PCR were higher than those in which symptoms were observed. These results indicated that PCR amplification of a PWB-specific DNA fragment is an effective tool for practical diagnose and that PWB is widely distributed in the Tohoku district of Japan.     

Attachment and infection to MA104 cells of avian rotaviruses require the presence of sialic acid on the cell surface

To determine the characters of receptors on target cells for avian rotaviruses, the receptors on MA104 cells for the pigeon rotavirus PO-13, the turkey rotaviruses Ty-1 and Ty-3, and the chicken rotavirus Ch-1 were analyzed. Pretreatment of MA104 cells with neuraminidase greatly reduced the infection by all of the four avian rotavirus strains. Binding of the cell-attachment protein, purified VP8 expressed in bacteria, of strain PO-13 to MA104 cells was also inhibited by pretreatment of cells with neuraminidase. These findings suggest that avian rotaviruses primarily utilize sialic acid-containing molecules as receptors on MA 104 cells.     

Studies on keeping-quality of vegetables and fruits. III. Changes in sugar and organic acid contents accompanying the chilling-injury of eggplant fruits

The mechanism of chiiling-injury in fruits of eggplants (Solanum melongena L. cultivar ‘Hyonaga’) was investigated by determining the sugar and organic acid content during cold storage and after exposure to low temperature for various periods. Fructose, glucose and maltose were detected, and totalled more than 96% of total sugar on the initial day. Total sugar content increased to 30% higher than the initial day after 2 days of cold storage, and after that decreased gradually, while in 20° C-stored fruits it decreased sharply. Fructose, glucose and maltose increased rapidly after transfer from 1° C to 20° C. Succinic, oxalic, malic and citric acid were detected before storage. Malic and citric acid were found to be the main organic acids. The ratio of malic/citric acid in fruits stored at 1°C decreased during storage, while after storage at 20°C it increased. The ratio in fruits after transfer from 1°C to 20°C increased rapidly during the time course. The ratio of malic/citric acid ranged from 6.0 to 6.8 in healthy fruits, while it was below 5.9 or above 7.3 in chilled fruits. It is suggested that according to this ratio the degree of chilling-injury of eggplant fruits might be foreseen.     

The first chemical enrichment in the universe and the formation of hyper metal-poor stars

The recent discovery of a hyper-metal-poor (HMP) star, with a metallicity Fe/H smaller than 1/100,000 of the solar ratio, together with one earlier HMP star, has raised a challenging question whether these HMP stars are the actual first-generation, low-mass stars of the universe. We argue that these HMP stars are second-generation stars formed from gases that were chemically enriched by the first-generation supernovae. The key to this solution is the very unusual abundance patterns of these HMP stars and the similarities and differences between them. We can reproduce these abundance features with core-collapse "faint" supernova models that include extensive matter mixing and fallback during explosions.