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91.
Detection of rabies viral antigens in non-autolysed and autolysed tissues by using an immunoperoxidase technique 总被引:2,自引:0,他引:2
The objectives of this study were to determine the potential of an immunoperoxidase technique involving the avidin-biotin complex (ABC) stain for the diagnosis of rabies in fresh tissues and compare it with other standard methods, including the fluorescent antibody test (FAT), haematoxylin and eosin and Seller's stain, and to investigate its capacity to detect rabies antigen in autolysed tissues. Samples of non-autolysed brain from 81 domestic and wild animals suspected of having rabies were examined. Rabies antigen was detected by FAT in 41 of these samples and Negri bodies were detected in 40 (97.6 per cent) of them by the immunoperoxidase technique, in 25 by haematoxylin and eosin and in 22 by Seller's stain. The sensitivity of the immunoperoxidase technique decreased as the tissues were left to autolyse; after two days it was 91.2 per cent, after four days 70.6 per cent, and after seven days 11.8 per cent. 相似文献
92.
We investigated the feminization of bluegill and black crappie by short periodic immersions in an aqueous estradiol‐17β (E2) solution. Bluegill fry (13.8 mm) were immersed in a 1 mg L?1 E2 solution every 5 days on three occasions over an 11‐day period. In the positive control treatment, the same cohort of fry was fed 200 mg kg?1 E2 diet for 45 days. Two different size (26.1 and 20.1 mm) but the same age (45 days old) black crappie fry were immersed in the same concentration of E2 solution for 5 h a day every 3–5 days on 10 occasions between 45 and 86 days post hatch. Both modes of E2 administration were effective to induce feminization in bluegill. Feeding fry E2 impregnated diet for 45 days yielded 99.3% female populations from a 38.8% female experimental population. Only three short‐time E2 immersions of fry over a much shorter treatment period also produced 76.9% female populations. E2 immersions had no effect on sex ratios of larger black crappie fry and produced variable levels of feminization (60–82% female) in smaller black crappie fry. Results of the present study showed that feminization in both species can be achieved by periodic immersions of fry in a 1 mg L?1 E2 solution with no adverse effects on survival. Yet, further researches on timing and intensity of immersion treatments are still needed for optimization of the procedures. 相似文献
93.
H. Filiz Ayyildiz Mustafa Topkafa FatmaNur Arslan Fatih Durmaz Semahat Kucukkolbasi Ismail Tarhan Huseyin Kara 《Water, air, and soil pollution》2012,223(7):3817-3830
This work assesses the use of immobilized humic acid (ImHA) onto aminopropyl silica (APS) as a sorbent for the removal and preconcentration of trace amounts of cobalt ions by on-line solid phase extraction (SPE) technique in the column system prepared in our laboratory. Different parameters, such as the effect of the pH, concentration, and flow rate, were studied and throughput was observed by a UV detector. All SPE steps were monitored by breakthrough curves used to visualize distribution of cobalt concentration between mobile and solid phase. The solutions collected from stripping steps were analyzed in atomic absorption spectrometer (AAS) and the amount of sorbed ions was calculated. Sorption characteristics were evaluated by using common adsorption isotherms and Scatchard plot analysis. From the obtained results, it was seen that sorption mechanism of cobalt ions were fitted to Langmuir model on a large scale and thought to be localized. Mean free energy (E?=?40.82?kJ?mol?1) calculated from D-R isotherm showed that chemical interactions are more effective than physical interactions. This investigation reveals a new, simple, environmentally friendly, and cost-effective method for removal and preconcentration of cobalt ions from aqueous solutions by a new aminopropyl silica-immobilized humic acid material. 相似文献
94.
This study was carried out to determine the prevalence of Cryptosporidium species in diarrhoeic lambs and investigate some risk factors in Kars province (Northeastern region of Anatolia) in Turkey.
Four hundred faecal samples were taken from the rectums of clinically diarrhoeic and aged to 1-month-old lambs from 34 sheep
farms in 20 villages in March-April 2007 and examined by using the modified acid-fast staining technique. The prevalence of
Cryptosporidium species was found as 38.8% (155/400). Cryptosporidium oocysts were detected in 90.0% (18/20) of villages and in 76.5% (26/34) of the sheep farms. Infection rates were detected
as: 44.4% (67/151) in 1-week-old lambs, 37.5% (39/104) in 2-week-old lambs, 40.0% (38/95) in 3-week-old lambs, and 22.0% (11/50)
in 4-week-old lambs. Farms classified according to their zoohygienic conditions and fine, average and bad conditioned farms
were contaminated with Cryptosporidium with the percentages of 14.7%, 20.6% and 41.2%, respectively. Clinical cryptosporidiosis was determined in 35.0% of the villages
(7/20) and in 29.4% of the sheep farms (10/34), Cryptosporidium oocysts were found in 81.3% of the lambs (91/112) in these farms. Cryptosporidiosis may be a major epidemiological significance
in lambs in Kars province, and suggests that naturally infected lambs may be reservoirs of Cryptosporidiosis infections for
calves even for humans too. 相似文献