黄颡鱼暴发性流行病病原的分离鉴定及其3种毒力基因检测

为查明黄颡鱼(Pelteobagrus fulvidraco)暴发性流行病的病原菌及其3种毒力基因的携带情况,以常规方法进行细菌分离,人工感染试验确定病原菌的致病性,API 20NE和16S rRNA基因序列分析进行细菌鉴定,PCR扩增法检测病原菌的3种毒力基因。结果显示,从发病症状典型的黄颡鱼肝脏中分离到1株嗜水气单胞菌(Aeromonas hydrophila)GXGP1,与嗜水气单胞菌标准株ATCC 7966T的同源性为99.9%,对黄颡鱼有很强致病力,是引起黄颡鱼暴发性流行病的病原菌,该菌携带有Aer、hly和ahp 3种毒力基因。     

养猪小区粪便资源化处理工艺设计

影响我国养猪小区健康发展的关键问题是养猪小区的环境控制,降水、污水、粪便混合排放,加剧了养猪小区的环境污染,使粪便资源化处理更加困难。根据现代养猪生产工艺流程,利用工程技术手段,设计了一个现代化示范型养猪小区粪便资源化处理工艺,首先解决天然降水与污水混合排放问题,其次实现污水与粪便有效分离,第三步通过充氧发酵、搅拌干燥最终实现养猪小区粪便资源化开发利用,解决捆扰我国养猪小区健康发展的环境控制问题。     

梨叶片中苹果褪绿叶斑病毒的引物原位标记检测

 选用带苹果褪绿叶斑病毒的香梨叶片,制备成适合进行原位RT-PCR的石蜡切片。设计特异引物,利用引物原位标记技术对切片组织中的病毒进行了检测研究,结果表明,PRINS-FITC染色法和PRINS-Rhodamine染色法均能获得良好的检测效果,有病毒部位分别显示绿色荧光和红色荧光,而且荧光出现的位置与原位RT-PCR检测的结果一致。由此证明引物原位标记技术可用于果树病毒的原位检测。     

Mechanism of mesenteric lymph reperfusion aggravates multiple organ injury in superior mesenteric artery occlusion shock rats

AIM: To explore the mechanism of mesenteric lymph reperfusion (MLR) aggravates multiple organ injury in superior mesenteric artery occlusion (SMAO) shock rats. METHODS: Male Wistar rats were randomly divided into 4 groups: in sham group, only anesthetization and operation were performed; in MLR group, occlusion of mesenteric lymphatics (ML) for 1 h followed by 2 h of reperfusion; in SMAO group, occlusion of superior mesenteric artery (SMA) for 1 h followed by 2 h reperfusion; in MLR+SMAO group, occlusion of SMA and ML for 1 h followed by 2 h of reperfusion. The homogenates of liver, kidney, myocardium and lung were prepared for determining the activities of free radical, nitric oxide, myeloperoxidase (MPO) and cell membrane ATPase. RESULTS: The MDA, NO contents and NOS, MPO activities of multiple organic homogenate in SMAO and MLR+SMAO group were higher than those in sham and MLR group, and these indexes in MLR+SMAO were increased significantly than those in SMAO group. The SOD and ATPase activities of muliple organic homogenate in SMAO and MLR+SMAO group were lower than those in sham and MLR group, and those in MLR+SMAO group was decreased obviously than those in SMAO group. CONCLUSION: The MLR enhances the multiple organ free radical injury, NO synthesis and release, PMN detention and decreases the activity of cell membrane ATPase, aggravating the major organs injury in SMAO shock rats. Intestinal lymphatic pathway plays an important role in the pathogenesis of SMAO shock.     

黄瓜耐弱光性的多元统计分析

 利用遮阳网模拟弱光条件, 调查18份黄瓜自交系苗期生长发育和生理生化指标, 采用多元统计分析方法评价黄瓜品系耐弱光性强弱。结果表明, 参试品系的13个指标在品系间存在显著差异, 利用因子分析将这些指标综合成5个主因子, 累计贡献率达85.62%。对旋转后的因子得分进行聚类分析, 供试品系可分为4类: 耐弱光材料M₂₂、M₄₃、M_a9; 较耐弱光材料M₈、M₁₁、M₃、M₄、M₃₄; 弱光中度敏感材料M₁₂、M₂₁、M₁₄、M₂、M_a1、M₂₈和弱光敏感材料M₁₀、M₄₄、M₃₂、M₃₆。经过弱光处理后耐弱光指数较大、光合速率降低幅度较小的品系耐弱光性强, 应结合耐弱光指数与光合、生长等因子综合进行黄瓜耐弱光性鉴定。     

甘蓝自交不亲和决定因子的体外表达和相互作用的检测

 S位点受体激酶(SRK)和S位点富含半胱氨酸蛋白/S位点蛋白11(SCR/SP11)分别为甘蓝自交不亲和(self-incompatibility, SI)信号传导的雌雄决定因子。为了深入研究两者的作用机理和进行人工调控,本研究以结球甘蓝ZQ为材料,利用pET·NusA融合蛋白表达系统,将包含SRK胞外域和跨膜域的mSRK蛋白和SCR蛋白在大肠杆菌BL21中融合表达,经SDS-PAGE电泳检测表达出融合蛋白大小分别约为116 kD和74 kD。进一步将两融合蛋白进行体外相互作用的检测,结果表明mSRK与SCR蛋白能够相互结合形成稳定的复合体,这为下一步实现SRK-SCR复合体聚合与解离的人为调控提供了技术平台。     

Jagged1 knocking down in endothelial cells accelerates PDGF induced proliferation and migration of vascular smooth muscle cells in rats

AIM: To investigate the effect of Jagged1 expression in endothelial cells (EC) on platelet derived growth factor (PDGF) induced proliferation and migration of vascular smooth muscle cells (VSMC) in rat.METHODS: Rat aorta EC was inoculated in the lower chamber and VSMC were in the upper chamber of the cell coculture system. Three groups were divided: control, sicontrol and siJagged1. The EC Jagged1 protein expression was assayed by Western blotting to evaluate small RNA interfering (RNAi) efficiency. After the cells were cocultured with PDGF for 24 h, the proliferation and migration of VSMC were respectively evaluated by ［3H］-TdR incorporation and migrating cells counting. Protein expression of α-SM-actin in VSMC was assayed by Western blotting. RESULTS: The Jagged1 protein expression in EC was significantly lower in siJagged1 group than that in control group (0.26±0.02 vs 0.67±0.02, P<0.05), and no statistic significance was observed between control and sicontrol groups. The VSMC ［3H］-TdR incorporation and migration were higher in PDGF +siJagged1 group than those in PDGF group {［3H］-TdR incorporation (23 074±2 702) counts·min-1·well-1 vs (16 442±1 803)counts·min-1·well-1, n=5, P<0.05; migration (27±4) cells/field vs (15±3)cells/field, n=5, P<0.05}. The α-SM-actin protein in VSMC was lower in PDGF + siJagged1 group than that in PDGF group (0.25±0.06 vs 0.49±0.04, n=3, P<0.05).CONCLUSION: Jagged1 knock down in rat EC accelerates PDGF induced proliferation and migration of VSMC. These results suggest that Jagged1 expression in EC plays an important role in maintaining VSMC contract phenotype and inhibiting VSMC overgrowth after arterial injury.     

早熟大白菜新品种中白62号的选育

中白62号是以矬菜类型早熟材料自交系B60533和青麻叶类型材料自交系B60532为亲本配制而成的早熟大白菜一代杂种。成熟期60 d(天)左右。株型紧凑,株高39 cm,开展度57 cm,外叶深绿色,少皱、有光泽,叶缘波褶小而浅,叶柄浅绿色。叶球直筒形,顶部舒心,球高35 cm,横径13 cm,球形指数2.8,叶球净质量1.6 kg左右。每667 m2净菜产量4 600 kg左右,高抗病毒病、霜霉病、黑腐病,球叶不易发生干烧心。质地脆嫩,品质优良,适宜在辽宁、河北、天津、北京、陕西、贵州等省市及山东、黑龙江、河南、山西等省的部分地区秋季种植。     

几个鸢尾新品种栽培研究

对几种在郑州地区新引进的德国彩花鸢尾进行了栽培研究。结果表明:不朽白和黑骑士生长较旺,花朵较大,但开花率低,花期较短,繁殖系数低;黄娃娃和血石长势中庸,花朵中等,开花率高,花期较长,繁殖系数也高;金娃娃与黄链长势中庸偏下,花朵较小,但花期较长,金娃娃繁殖系数中等,黄链繁殖系数最低;紫边白植株矮小,长势较弱,花朵也小,但花期较长,繁殖系数较低。无论任何品种在郑州地区均能良好生长,且其生长、开花、繁殖均与土壤肥沃、疏松、排水良好呈正相关。     

鸡粪高效降解微生物菌剂富集培养新法

以新鲜鸡粪为主要培养基,自然腐熟鸡粪(处理1),菌剂腐熟鸡粪和土壤(处理2),菌剂腐熟鸡粪(处理3)为富集样品连续5批堆肥驯化培养,使微生物菌群经历不同的生长环境,达到优胜劣汰的效果。处理1在5批富集培养中较处理2和处理3有较好的堆肥效果,说明其中有降解性能好的优势菌群,将其筛选出扩大培养,再回接到新鲜鸡粪中,将会快速、有效地降解新鲜鸡粪,达到良好的堆肥效果。