离心风机串联吸气特性的试验研究

离心风机是最常用风机的一种，广泛应用于国民经济的各个领域。为此，在离心风机单机吸气性能试验的基础上，进行了离心风机串联吸气性能试验，得出了串联吸气性能与单机吸气性能的关系，以及适于串联的工况条件，为串联风机的参数选择提供了试验依据。     

利用振动特性诊断流程泵的运行工况

用所建立的用于描述振动强度的范数说明了泵在偏离原设计工况运行时会导致泵轴向力和径向力的增大。将流程泵叶轮叶片和压水室的隔舌看作旋涡发生体，在不同运行工况下在叶片后缘以及隔舌两侧串联发生的卡门涡街的发射频率是不同的。采用加速度传感器采集的振动信号正反应了这种工况变动。通过对振动信号进行基于EMD的能量算子解调以及频谱分析，发现得到的最大幅值处的频率就是叶轮叶片的通过频率。试验表明，得到的次大幅值处的频率就是卡门涡街的发射频率，可根据该频率处幅值的相对大小来判断泵是否在合理的工况内运行。     

灌溉水利用系数测定方法研究

通过开展对灌溉水利用系数资料的搜集、整理并对灌区进行实地调研,在了解目前灌区灌溉水利用系数测量中存在问题及原因的基础上,对灌区灌溉水利用系数测量计算方法进行了分析研究,提出了测定灌溉水利用系数的综合测定计算方法。该方法既克服了传统测量方法中工作量大,需要大量人力、物力才能完成的缺点,又弥补了只测量典型渠段而引起较大误差的不足,而且能反映出灌区渠系用水情况、灌溉工程质量及灌溉用水管理水平等。为灌区今后经常性地测量符合实际的灌溉水利用系数及指导灌区节水工程改造等提供了一种切实可行的计算方法。并结合实例进行了应用分析。     

基于局部综合法的非零正传动弧齿锥齿轮副设计

利用局部综合法对非零正传动弧齿锥齿轮副进行大重合度加工参数设计,克服了非零正传动由于啮合角增大而使得重合度降低的不足。通过实例与常规设计的结果进行仿真与应力分析对比,结果表明,在同样的载荷条件下,新设计比常规设计的小轮最大齿根拉伸应力减小27.6%,最大齿根压缩应力减小23.5%,最大齿面接触应力减小6.5%,同时大轮的齿根应力也有所降低,使得齿轮副具有更高的寿命和可靠性。     

杨凌区降雨对作物生长适应性的模糊分析

依据模糊数学理论 ,采用模糊综合评判方法 ,从降雨“适量”、“适时”和“有效程度”3个指标出发 ,就杨凌区降水资源对农作物生长发育适应性做了评价分析 ,并提出降雨对作物生长的适宜度模型。通过实地调查与计算分析 ,该模型较好地反映了杨凌区主要作物对该区降雨特征的适宜能力 ,同时也较好地反映了本区降雨资源的地域特征 ,为该地区农作物合理布局决策提供了科学依据     

柴油机机体的模态分析与减振设计

采用 ANSYS有限元分析软件 ,对一直列 6缸柴油机机体进行了模态分析 ,并与实验模态分析结果相比较 ,验证了计算模型的正确性。在该模型的基础上 ,从增加刚性和减少质量方面进行了研究 ,结果表明 ,铝制机体以及梯形框架结构和加强板结构均可减小机体的振动 ,进而降低发动机的噪声。     

农村小水电站机组增容改造的方法

我国在20世纪70年代兴建的小水电站已运行了几十年，随着新技术与新材料的应用，对老电站进行增容改造，提高电站的调峰能力是提高电站和县电网经济效益的关键措施之一。介绍了机组增容改造的方法和实际应用中应注意的问题。     

Construction of mouse ESC line stably expressing Pdx1 by Tet-On system

AIM To construct the mouse embryonic stem cell （ESC） line with stable pancreatic and duodenal homeobox 1 （Pdx1） expression by Tet-On system, which may lay a foundation for further research on the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells. METHODS The Pdx1-overexpressing lentiviral vector with green fluorescent protein marker and puromycin resistance was constructed by Tet-On system and was used to infect the mouse ESC. The cells were divided into 3 groups： blank control group （ESC group）, empty lentivirus control group （PDX1^- ESC group） and Pdx1 lentivirus transfection group （PDX1⁺ ESC group）. Flow cytometry was used to detect the transfected cells after screening by doxycycline （DOX）. The function of Tet-On system and the expression of Pdx1 gene were detected. The transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were sorted by flow cytometry, and constructed ESC line with stable expression of Pdx1 and negative control ESC line were verified. RESULTS （1） The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were 90.72% and 94.01% after screening by DOX, respectively. The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group was 97.84% and 98.13% after sorting by flow cytometry, respectively. （2） With DOX, green fluorescence was observed in PDX1^- ESC group and PDX1⁺ ESC group. The mRNA and protein expression of Pdx1 was significantly increased in PDX1⁺ ESC group （P<0.05）. Without DOX, no green fluorescence was observed in the cells of the 3 groups, and no significant difference in the mRNA and protein expression of Pdx1 was observed （P>0.05）. （3） After 3 months of cryopreservation, the cell lines still survived in resuscitation culture and were regulated by DOX. CONCLUSION Using Tet-On system, the mouse ESC line with inducible Pdx1 expression were successfully established and could be used as an effective cell model to research the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells.     

MicroRNA-9-5p mediates sympathetic overactivity by targeting KCNN3 in paraventricular nucleus of rats with T2D

AIM To investigate whether microRNA-9-5p （miR-9-5p） mediates sympathetic overactivity by targeting KCNN3 （potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3） gene,which encoded small-conductance calcium-activated potassium channel 3 （SK3） protein, in paraventricular nucleus （PVN） of rats with type 2 diabetes mellitus （T2D）. METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus （rAAV）-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control （DC） group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus （DM） rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced （P<0.05）. However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.     

桃新品种‘秋露蜜’的选育

‘秋露蜜’是从‘国庆’桃实生后代中选出的浓甜晚熟桃新品种。平均单果重248.3 g,果面鲜红,果肉乳白色,甘甜,品质上等,平均硬度达15.30kg/cm²,可溶性固形物含量19.08%。在晋南地区,果实9月上中旬成熟,果实发育期162 d左右。离核。