Effect of γ (Gamma)-radiation on the physico-mechanical properties of grafted jute fabric reinforced polypropylene (PP) composites

The bleached jute fabric (BJF) reinforced polypropylene (PP) composites with various contents of acrylic acid (AA)-treated BJF and un-AA-treated BJF were fabricated by compression moulding method at 190 °C. The AA-grafted BJF reinforced PP composites were then irradiated by γ-ray at various doses. The mechanical properties of neat PP (N-P), ungrafted-BJF and PP composites (UG-BJFPC), AA-grafted-BJF and PP composites (AA-BJFPC) and γ-ray cum AA-grafted-BJF and PP composites (γAA-BJFPC) show maximum tensile strength (TS) of 30, 46, 47 and 51 MPa, maximum flexural strength (FS) of 34, 49, 50 and 54 MPa and maximum Young’s modulus (E) of 280, 428, 436, and 680 MPa, respectively. The increase of TS, FS and E from UG-BJFPC are 2 %, 2 %, and 2 % for AA-BJFPC and 11 %, 10 % and 59 % for γAA-BJFPC. The TS, FS and E are found to increase with radiation dose up to 500Krad and then decrease. The water absorption (WA) for UG-BJFPC, AA-BJFPC and γAA-BJFPC is respectively about 14, 10 and 9 %, indicating a gradual development of hydrophobic character of the composites first by AA-treatment and then by γ-ray-treatment. AA treatment on jute fabric and gamma irradiation on composite result in significant change of morphology of the jute fabric composites surface and better mechanical bonding between fabric and polymer matrix, as a result improved mechanical properties are found.     

Assessment of molecular genetic diversity of 384 chickpea genotypes and development of core set of 192 genotypes for chickpea improvement programs

Genetic Resources and Crop Evolution - Cicer arietinum L. (chickpea) is one of the most significant legume crops domesticated in the Fertile Crescent. This study was aimed to characterize a diverse...     

Rhizosphere microbiomes can regulate plant drought tolerance

Beneficial root-associated rhizospheric microbes play a key role in maintaining host plant growth and can potentially allow drought-resilient crop production. The complex interaction of root-associated microbes mainly depends on soil type, plant genotype, and soil moisture. However, drought is the most devastating environmental stress that strongly reduces soil biota and can restrict plant growth and yield. In this review, we discussed our mechanistic understanding of drought and microbial response traits. Additionally, we highlighted the role of beneficial microbes and plant-derived metabolites in alleviating drought stress and improving crop growth. We proposed that future research might focus on evaluating the dynamics of root-beneficial microbes under field drought conditions. The integrative use of ecology, microbial, and molecular approaches may serve as a promising strategy to produce more drought-resilient and sustainable crops.     

Build up of patches caused by Rhizoctonia solani

Rhizoctonia solani is a complex species that is composed of different anastomosis groups (AG). Although these different AGs show differences in their host ranges, generally R. solani is a phytopathogenic species with a wide spectrum of hosts. It has the ability to grow as a saprotroph, which further complicates its behaviour as a parasite. The losses caused by R. solani are very important and need a sustainable management strategy. The patchy appearance of the disease caused by this pathogen is well-known. The patches show within and between season dynamics. The factors which affect the spread of the disease can be grouped into three main categories: host plant, pathogen and environment. However, each of the categories in its detail may depend on or react with the other categories. There are a number of factors that may be involved in dynamics of patches. These potential mechanisms are discussed. It is essential to know about the mechanisms involved to develop an effective control strategy. Although more work is needed to investigate different mechanisms of parasitism deployed by different AGs in different hosts, it seems that many mechanisms external to the host are operating at the same time which necessitates an integrative research approach to study and control the diseases caused by R. solani.     

A Preliminary Comparison of Plasma Fibrinogen Concentrations,Leukocyte Numbers and Erythrocyte Sedimentation Rate as Non-Specific Indicators of Inflammatory Conditions in Buffalo (Bubalis Bubalis)

Zargham Khan, M., Muhammad, G., Umar, A. and Ali Khan, S., 1997. A preliminary comparison of plasma fibrinogen concentrations, leukocyte numbers and erythrocyte sedimentation rate as non-specific indicators of inflammatory conditions in buffalo (Bubalis bubalis). Veterinary Research Communications, 21 (4), 265-271The plasma fibrinogen concentration (Fib), total leukocyte count (TLC), neutrophil, lymphocyte and monocyte numbers, and the erythrocyte sedimentation rate (ESR) were determined in 153 buffaloes suffering from different clinical conditions. Fib increased significantly (p < 0.05) in chronic mastitis, pyrexia, pyometra, cutaneous abscesses, tail gangrene and acute indigestion, whereas in most of the other conditions studied it varied non-significantly. TLC increased significantly in chronic mastitis, pyrexia, endometritis, cutaneous abscesses and infected skin wounds. An increase in neutrophils was associated with an increased TLC. Numbers of lymphocytes varied non-significantly in most of the conditions. Monocytes decreased significantly in most of the acute conditions. ESR was significantly elevated in all clinical conditions. Significantly increased mean Fib values in the different conditions varied from 703 ± 119 to 725 ± 140 mg/dl, while the maximum individual value was 1510 mg/dl in a case of cutaneous myiasis. The significantly increased mean TLC ranged from 9.48 ± 2.91 to 11.1 ± 3.5 × 103/µl, while it was 21.7 × 103/µl in a case of meningitis. ESR values in sick buffaloes varied from 57 to 111 mm in the first hour.     

Efficient production of doubled haploid Brassica napus plants by colchicine treatment of microspores

Summary The effect of colchicine on isolated microspore cultures of Brassica napus was evaluated in order to combine a positive effect of colchicine on the induction of embryogenesis with the possibility to induce chromosome doubling at an early developmental stage, thus avoiding the production of haploid or chimeric plants. Colchicine was added to the culture medium immediately after isolation of B. napus microspores. The cultures were incubated from 6 to 72 h with various concentrations of colchicine. Samples were taken from the regenerating embryoids after 6 weeks for ploidy determination by flow-cytometry.The highest diploidization rate was obtained after a 24 h treatment of microspores with 50 mg/l colchicine, leading to 80–90% diploid embroids. A concentration of 100 mg/l colchicine applied for the same duration resulted in a lower diploidization rate (76–80%). Treatment durations of 6 h were not long enough to induce a high rate of diploidization, whereas the application of 10 mg/l for 72 h was also very effective.A sample of the plants regenerated from the colchicine treated microspores was transferred to the greenhouse. The plants looked similar to normal diploid rapeseed plants and showed reasonable pod and seed set. Thus, an additional generation for seed increase in the greenhouse is rendered unnecessary. The advantage of applying a minimum volume of colchicine under controlled in vitro conditions means a considerable saving of time and labour in DH-breeding programs.     

Stability of RAPD markers for determining cultivar specific DNA profiles in rye (Secale cereale L.)

Summary Cultivar specific DNA profiles in rye were revealed by polymerase chain reaction (PCR) using randomly amplified polymorphic DNA (RAPD) sequences. Ten base primers were used for the amplification of genomic DNA of rye cultivars by PCR. RAPD analysis was found to be reproducible among samples between PCR runs. When amplification profiles of different rye cultivars were compared using various primers, the overall profiles were cultivar specific. However, not all primers revealed polymorphisms. These primers appear to amplify conserved sequences in all rye cultivars. Intracultivar studies were conducted on two of the cultivars. In the cultivar Imperial, no polymorphisms were observed among ten plants analyzed with five primers. In the cultivar Balboa, polymorphisms were observed among fifty plants with four of the ten primers analyzed. Despite the small amount of intracultivar variability, RAPD analysis has the potential to be a rapid and reliable method of cultivar identification in this outcrossing species.     

Genetic analysis of flowering and maturity time in high latitude spring wheat

Due to the short growing season in the high northern latitudes, the development of early maturing spring wheat (Triticum aestivum L.) cultivars is important to avoid frost damage which can lower production and quality. We investigated earliness of flowering and maturity, and some associated agronomic traits, using a set of randomly selected high northern latitude adapted spring wheat cultivars (differing in maturity) and their F₁ and F₂ crosses made in a one-way diallel mating design. The parents, and their F₁ and F₂ crosses were evaluated under field conditions over 2 years. Anthesis and maturity times were controlled by both vernalization response and earliness per se genes, mainly acting additively. Non-additive genetic effects were more important in controlling grain fill duration, grain yield and plant height. Additive × additive epistatic effects were detected for all traits studied except time to anthesis. Segregation analyses of the F₂ populations for time to anthesis indicated the presence of different vernalization response genes. Molecular genetic analyses revealed the presence of Vrn-A1 and Vrn-B1 genes in the parental cultivars. Narrow-sense heritability was medium to high (60–86%) for anthesis and maturity times but low to medium (13–55%) for grain fill duration, plant height and grain yield. Selection for early flowering/maturity in early segregating generations would be expected to result in genetic improvement towards earliness in high latitude spring wheats. Incorporation of the vernalization responsive gene Vrn-B1 in combination with vernalization non-responsive gene Vrn-A1 into spring wheats would aid in the development of early maturing cultivars with high grain yield potential for the high latitude wheat growing regions of the northern hemisphere.