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排序方式: 共有100条查询结果,搜索用时 46 毫秒
71.
Comparative analyses of canine distemper viral isolates from clinical cases of canine distemper in vaccinated dogs 总被引:10,自引:0,他引:10
Lan NT Yamaguchi R Inomata A Furuya Y Uchida K Sugano S Tateyama S 《Veterinary microbiology》2006,115(1-3):32-42
Sequence and phylogenetic analyses of three isolates of canine distemper virus (CDV) isolated from three dogs with a vaccination history were compared with the same analyses of vaccine virus isolated from a vaccine used for dogs. The three dogs showed clinical signs of a recent major type of CD in Japan, including oculonasal discharge and diarrhea, and pathological findings including non-suppurative encephalitis, pneumonia, mild gastroenteritis and lymphoid depletion. Inclusion bodies were in the stomach without inflammation and encephalitis was without clinical signs. One of the highest titers of CDV in different organs of the three dogs was commonly systemic lymphatic organs, including the spleen, lymph nodes and tonsils. New isolates of CDV joined to the clades of the Asia 1 group that is far from the vaccine group. These results surely indicate that wild strains of CDV from dogs with a vaccination history were not reversed vaccine virus, and that the dogs showed characteristics of recent CD in Japan. 相似文献
72.
Misaki Maeda Takashi Hosoya Koichi Yoshioka Hisashi Miyafuji Hiroyuki Ohno Tatsuhiko Yamada 《Journal of Wood Science》2018,64(6):810-815
Vanillin is one of the industrially important compounds that can be produced from lignin. This study presents production of vanillin and vanillic acid (oxidized form of vanillin) through aerobic oxidation of Japanese cedar (Cryptomeria japonica) at 120 °C for 72 h in aqueous alkali solutions with several Bu4N+ and OH? concentrations (1.25, 2.50, and 3.75 mol/L), where Bu4N+ is an enhancer of the vanillin formation reported in our previous study. The concentrations of Bu4N+ and OH? were adjusted by the additions of Bu4NCl and solid NaOH into the base medium Bu4NOH·30H2O, which forms 1.25 mol/L aqueous solution of Bu4NOH at the elevated temperature. Vanillin and vanillic acid were produced with the maximum yields of 21.0 and 1.7 wt% (lignin-base), respectively, at the 1.25 mol/L Bu4N+ and 3.75 mol/L OH? concentrations. This vanillin yield is close to that obtained by the alkaline nitrobenzene oxidation (26.5 wt%), indicating significantly high selectivity of our lignin degradation with Bu4N+ toward vanillin formation. We also proposed a novel Bu4NOH·30H2O-free reaction medium, where Bu4NOH·30H2O as the base medium were substituted with an aqueous solution of Bu4NCl and NaOH to avoid using expensive Bu4NOH·30H2O. The treatment of the Japanese cedar with this alternative medium exhibited the moderately decreased vanillin yield of 14.6 wt%, which is, however, much higher than the vanillin yield obtained with a simple 1.25 mol/L NaOH solution. 相似文献
73.
Htet Wai Wai Kyaw Kenichi Tsuchiya Masaru Matsumoto Kazuhiro Iiyama Seint San Aye Myo Zaw Daisuke Kurose Mitsuo Horita Naruto Furuya 《Journal of General Plant Pathology》2017,83(4):216-225
In 2013 and 2014, an extensive survey of bacterial wilt in Myanmar was performed, and 70 strains of Ralstonia solanacearum (Rs) were collected from wilting plants of tomato, potato, chili and eggplant. Myanmar Rs strains were characterized by traditional and molecular methods. Polymerase chain reaction (PCR) test using Rs-specific primer set amplified one specific band (281-bp) from template DNA of all strains. Pathogenicity tests on the four solanaceous plants differentiated the strains into six pathogenic groups. Biovar determination tests showed that biovar 3 strains predominated (63%) among all Rs strains. Biovar 4 strains (7%) were obtained from both tomato and chili strains, whereas biovar 2 (30%) strains were isolated only from potato. Multiplex-PCR analysis indicated that tomato, eggplant and chili strains belonged to phylotype I, whereas potato strains comprised phylotype I and phylotype II. Strains in phylotype I, which was suggested to have originated from Asia, were the most prevalent in all surveyed areas. Phylogenetic analysis based on the endoglucanase (egl) gene sequences revealed that Myanmar strains partitioned into two major clusters that corresponded to phylotype I and II. Strains in phylotype I were further divided into seven subclusters, each corresponding to a distinct sequevar (15, 17, 46, 47, 48, unknown 1 or unknown 2). All strains in phylotype II belonged to sequevar 1. This is the first comprehensive report of the presence of diverse Rs strains in Myanmar. 相似文献
74.
75.
Hiromitsu Furuya Keisuke Tubaki Tsutomu Matsumoto Shin-ichi Fuji Hideki Naito 《Journal of General Plant Pathology》2007,73(1):8-14
Roots of rice plants grown in paddy fields in a transplant culture system were collected seven times between 8 and 22 weeks
after transplanting, for two crop seasons. Rice seedlings grown in a potting medium amended with the collected roots were
significantly shorter than those grown in the same medium either without the addition of the roots or amended with pasteurized,
collected roots indicating that seedlings were inhibited by heat-labile microorganisms on the collected rice roots. Ninety-five
and 172 pure cultures of Pythium spp. and fungi, respectively, were isolated from the rice roots collected 5 or 7.5 weeks after transplanting in the fields.
Among these microorganisms, Pythium aristosporum inhibited seedling growth in greenhouse experiments, and Acremonium sp., Alternaria sp., Epicoccum nigrum, Fusarium sp., Massarina sp., Penicillium spp., Rhinocladiella sp., Stemphylium sp., Trichocladium sp., and several unidentified fungi inhibited seminal root growth in in vitro experiments. These microorganisms might be
involved in the inhibition of seedling growth in soils amended with the rice roots collected from the paddy field. Thus, roots
of rice plants at the middle stage of growth transplanted into paddy fields can harbor pathogenic or deleterious fungi or
Pythium sp(p). The effects of these microorganisms on rice growth in paddy fields are discussed. 相似文献
76.
Isolation of Encephalitozoon cuniculi using primary tissue culture techniques from a rabbit in a colony showing encephalitozoonosis 总被引:1,自引:0,他引:1
Furuya K Fukui D Yamaguchi M Nakaoka Y Bando G Kosuge M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(2):203-206
Encephalitozoon spores were isolated in a primary tissue culture of the kidneys from an encephalitozoonosis-suspected rabbit in a municipal zoo in Hokkaido. The isolated spores were morphologically characteristic of microsporidial ones in chromotrope stain, and proven to be E. cuniculi by a polymerase chain reaction (PCR) with a species-specific primer set and by direct DNA sequencing of the PCR products. 相似文献
77.
Noriko Furuya Keiichiro Matsukura Kenta Tomimura Mitsuru Okuda Shin-ichi Miyata Toru Iwanami 《Journal of General Plant Pathology》2010,76(2):122-131
In Japan and Southeast Asia, ‘Candidatus Liberibacter asiaticus’ (Las) is the dominant causal agent of citrus greening (huanglongbing) disease. Using PCR techniques,
we determined the 11168-nucleotide sequence of the ψserA-trmU-tufB-secE-nusG-rplKAJL-rpoB gene cluster and the flanking regions for 51 Japanese, four Taiwanese, four Indonesian, and three Vietnamese isolates of
Las. The sequence is identical in 62 isolates collected from Japan, Taiwan, Indonesia, and Vietnam, except for nucleotide
substitutions at 11 positions. Some Las isolates from Sakishima Islands near Taiwan had unique nucleotide mutations, but all
Las isolates around Okinawa Main Island were homologous. On the basis of the pattern of single nucleotide polymorphisms (SNPs)
of the 11 nucleotide substitutions, the 62 Las isolates from Japan, Taiwan, Indonesia, and Vietnam could be divided into 12
pattern groups, and the 51 Japanese isolates consisted of six patterns. The results suggested that one unique genetic group
is dominant around Okinawa Main Island, whereas several different are commonly distributed around islands near Taiwan. 相似文献
78.
Michiko Hayano Naoki Horikawa Tsuneo Kuwagata Jun Furuya Yasushi Ishigooka Toshihiro Hasegawa Tomoyuki Taniguchi Akira Shimizu Hitoshi Toritani Minoru Tada Takao Masumoto 《Paddy and Water Environment》2008,6(1):15-23
This paper introduces the Agriculture, Forestry and Fisheries Research Council of Japan (AFFRC) model, an integrated model
that predicts future rice production in the Mekong River basin by taking into account the effect of global warming on both
the water cycle and the rice economy. The model focuses especially on the water balance of paddy fields for different farmland
water use systems. We defined six categories of irrigated paddies and three categories of rain-fed paddies on the basis of
their systems of water usage. We included a process-based model to predict future rice production, accounting for daily changes
in available water resources such as precipitation. Many models of crop production treat rice in the same way as other crops;
the particular characteristics of rice farming are considered in more detail in our model. Our results show that it is possible
to estimate future rice production in the Mekong River basin by taking into account changes in available water, and to model
the resultant effects on the grain market. 相似文献
79.
Apparent digestibility of soybean coproducts in extruded diets for Nile Tilapia,Oreochromis niloticus
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L.V.O. Vidal T.O. Xavier L.B. de Moura T.S. Graciano E.N. Martins W.M. Furuya 《Aquaculture Nutrition》2017,23(2):228-235
Soybean coproducts are important protein sources in aquaculture because of their amino acid profile. Tilapia can be reared on soybean coproducts as the only source of dietary protein without compromising growth. This study estimated apparent digestibility coefficients for five soybean coproducts for juvenile Nile tilapia, Oreochromis niloticus, fed with extruded diets. A reference diet and test diets consisting of 70% reference diet and 30% coproduct ingredient were formulated, along with 5.0 g kg?1 chromic oxide (Cr2O3) as a marker. Apparent digestibility coefficients (ADC) for dry matter (ADCDM), gross energy (ADCGE) and crude protein (ADCCP) for soybean coproducts were estimated as 84.2–88.4%, 86.9–91.1% and 96.8–97.9%, respectively. ADCDM and ADCCP values did not vary among the ingredients, but low‐protein soybean meal exhibited lower ADCGE values than did soybean protein concentrate. ADCs of all amino acids were >95%, and mean ADCs for amino acids were similar to the values observed for ADCCP. Soybean coproducts had good nutritional value in extruded diets fed to tilapia. Methionine, histidine and valine were the limiting amino acids in all evaluated ingredients. 相似文献
80.
Watabe Shugo Ikeda Daisuke Mashiro Takaki Kagetakubo Yuko Takahashi Yoshihiro Uemura Misaki Mizusawa Nanami Koyama Hiroki Yasumoto Ko Jimbo Mitsuru Kan-no Nobuhiro Ueda Tomohiro Matsuoka Yoko Ueki Nobuhiko Wan Jianrong 《Fisheries Science》2020,86(4):711-719
Fisheries Science - Complementary DNA encoding a part of myosin heavy chain was cloned from fast skeletal muscle of Japanese codling Physiculus japonicus. Japanese codling, a frequent catch off the... 相似文献