阿鲁科尔沁旗紫花苜蓿需水规律与灌溉定额

为了给阿鲁科尔沁旗紫花苜蓿(Medicago sativa)灌溉提供科学依据,利用1984-2013年30年气象数据,采用联合国粮农组织推荐的彭曼-蒙特斯公式法,研究了阿鲁科尔沁旗紫花苜蓿需水规律和灌溉定额。结果表明,阿鲁科尔沁旗紫花苜蓿第1茬、第2茬、第3茬、第4茬、生长季、非生长季和全年需水量分别为221、187、169、179、755、70和825mm,需水强度分别为4.3、4.7、4.1、2.5、3.7、0.4和2.3 mm·d~(-1),灌溉需水量分别为194、118、66、131、508、56和564mm,灌溉定额分别为228、139、78、154、598、66和664 mm。     

黄芪注射液对犬心脏双向变时作用的初步观察

为了观察黄芪注射液对正常犬和二尖瓣关闭不全病理模型 (MR)犬的变时作用 ,并探讨其机制 ,在异氟烷吸入麻醉下 ,对正常及外科方法制作的犬亚急性二尖瓣关闭不全模型 (术后 5 0天后 )一次性静脉推注含生药 2 g/ m L的黄芪注射液 1 .2 5 m L/ kg,通过体表心电图测定心率和 PQ、QRS、RR、QT,并计算 QTC(QT/ RR)间期。在正常犬 ,开始注射黄芪注射液后 ,心率出现先减少后恢复再持续减少和显著变化。 MR模型犬开始注射液后 ,心率出现先升高后恢复再持续升高的显著变化。 MR模型犬的心率减慢变化与 RR、PQ、QT、QTc呈高度显著负相关 (P<0 .0 1 )。表明黄芪注射液对正常比格犬 ,具有加快心率的正性变时作用。对二尖瓣闭锁不全 (MR)模型犬具有减缓心率的负性变时作用。黄芪注射液的变时作用似与机体的机能状态有关 ,呈现双向调节作用。     

添加微生物植酸酶对中国绍兴麻鸭产蛋性能的影响

试验选用 1 2 0 0只 2 6周龄的中国绍兴麻鸭 ,随机分为 5个处理组 ,每个处理组 2 4 0只鸭 ,分为 4个重复 ,试验期 70天。观测微生物植酸酶替代日粮中部分磷酸氢钙对产蛋鸭生产性能的影响。结果表明 ,添加 3 0 0FTU/Kg植酸酶替代产蛋鸭高峰饲料中 70 %磷酸氢钙时就可保持产蛋鸭的生产性能不变。随着植酸酶添加量的增加 ,产蛋鸭的生产性能越好 ,添加 4 0 0FTU/Kg植酸酶替代产蛋鸭高峰饲料中70 %磷酸氢钙时 ,产蛋率极显著优于对照组 (P <0 .0 1 )。添加 50 0FTU/Kg植酸酶替代产蛋鸭高峰饲料中70 %磷酸氢钙时 ,产蛋率极显著地优于对照组 (P <0 .0 1 )、饲料转化率显著地优于对照组 (P <0 .0 5) ,采食量各组之间差异不显著。由此可见 ,植酸酶可以用于产蛋鸭高峰饲料中 ,且以添加 4 0 0～ 50 0FTU/Kg植酸酶活性单位的饲养效果最好     

Effect of Fermentation Bed with Spent Mushroom Substrate of Pleurotus eryngii on Weaning Piglets

[Objective] The paper was to investigate effects on fermentation bed temperature,growth performance,diarrhea rate and digestive en-zyme activity of weaning piglets by using spent mushroom substrate of Pleurotus eryngii as padding.[Method] A total of 120 weaning piglets(Duroc × Landrace ×Yorkshire) with average initial body weight of(8.0 ±0.5)kg were allocated to five dietary treatments in a randomized complete block design for 42 d,each of which was replicated three times with eight piglets per replicate(half male,half female).The padding for control group was(50% sawdust +50% rice husk);experimental group Ⅰ 100% spent mushroom substrate;experimental group Ⅱ(15% sawdust +15% rice husk +70% spent mushroom substrate);experimental group Ⅲ(25% sawdust +25% rice husk +50% spent mushroom substrate);experimental group Ⅳ(35% sawdust +35% rice husk +30% spent substrate).[Result] There was no significant difference in surface temperature of fermentation bed between experimental groups and control group(P0.05).Compared with the control group,the temperature under 20 cm of fermentation bed in ex-perimental groups I,Ⅱ and Ⅲ increased significantly(P0.05).Except for experimental group Ⅳ,other three experimental groups had higher aver-age daily gain(P0.05) and experimental group Ⅰ had higher average daily feed intake(P0.05) compared to the control group.The diarrhea rate and mortality of weaning piglets in experimental groups Ⅱ,Ⅲ and Ⅳ were significantly decreased compared with the control group(P0.05).Compared with the control group,other three experimental groups had higher digestive enzyme activity in duodenal contents except for experimental group Ⅳ(P0.05).[Conclusion] Spent mushroom substrate of P.eryngii can be used as fermentation bed padding,and the optimal proportion was experimental group Ⅲ.     

鸭疫里氏杆菌吉林分离株camp基因的克隆、表达及间接ELISA检测方法的建立

为建立鸭疫里氏杆菌(RA)的血清学快速检测方法,本研究根据GenBank中登录的camp基因序列设计引物,以吉林RA分离株JL-1的基因组DNA为模板,通过PCR扩增camp基因,并构建pET-camp重组表达质粒,转化至E.coli BL21 (DE3)中诱导表达出分子量约为37 ku重组蛋白,westemblot分析结果表明该蛋白能够与血清1、2、10、11和17型RA全菌体阳性血清发生特异性反应.以纯化的重组蛋白为包被抗原建立间接ELISA方法,结果显示其具有良好的特异性和重复性,与RA超声裂解抗原ELISA方法的符合率达到77.5％.研究结果进一步验证了Camp蛋白具有良好免疫原性,并且为RA多种血清型共同抗原,在RA的检测及亚单位疫苗开发中具有良好的应用价值.     

Characterization of culture supernatant proteins from Brucella abortus and its protection effects against murine brucellosis

In this study, we characterized the secreted proteins of Brucella abortus into the enriched media under the bacterial laboratory growth condition and investigated the pathogenic importance of culture supernatant (CS) proteins to B. abortus infection. CS proteins from stationary phase were concentrated and analyzed using 2D electrophoresis. In MALDI TOF/TOF analysis, more than 27 proteins including CuZn SOD, Dps, Tat, OMPs, Adh, LivF, Tuf, SucC, GroEL and DnaK were identified. Cytotoxic effects of CS proteins were found to increase in a dose-dependent manner in RAW 264.7 cells. Upon B. abortus challenge into phagocytes, however, CS proteins pre-treated cells exhibited lower bacterial uptake and intracellular replication compared to untreated cells. Immunization with CS proteins induced a strong humoral and cell mediated immune responses and exhibited significant higher degree of protection against virulence of B. abortus infection compared to mice immunized with Brucella broth protein (BBP). Taken together, these results indicate that B. abortus secreted a number of soluble immunogenic proteins under laboratory culture condition, which can promote antibody production resulted in enhancing host defense against to subsequently bacterial infection. Moreover, further analysis of CS proteins may help to understand the pathogenic mechanism of B. abortus infection and host–pathogen interaction.     

师范生免费教育政策下的英语专业学生英语学习动机类型

采用问卷调查并结合半结构式访谈,探讨在师范生免费教育政策的条件下英语专业学生的动机倾向性,尝试归纳出动机类型,旨在为师范生免费教育相关政策的推广以及高等师范免费教育的培养模式提供一定的借鉴。此次调查的受试学生为402名西南大学外国语学院2007年9月入学的首批英语专业免费师范生。用SPSS13．0对问卷调查后所得数据进行了因子分析,归纳出八种动机类型：个人发展动机,兴趣动机、社会责任动机、文化融合动机、家庭责任动机、政策动机、专业观念动机、证书动机。调查的结果反映出师范生免费教育政策给他们的学习动机带来了直接或间接的影响。     

福建省牛病毒性腹泻病的血清学调查

为了了解福建省牛感染牛病毒性腹泻(BVDV)的情况,本中心对福建省9个地区2006-2008年的15个非免疫牛场和56个散养户共计511份样品进行了该病的检测。结果规模场BVDV血清学阳性一直保持在较高的水平,年平均阳性率维持在80%以上,散养户BVDV血清学阳性,2006、2007、2008年的血清学阳性分别为12.3%,21.4%和18.5%。调查表明,我省规模牛场感染BVDV较为严重,散养户感染BVDV的数量在逐年增加。     

当事人同时发生二种违法行为的案件处理与鉴别分析

1案情概述2007年11月22日,因有人举报本市某镇一家肉兔收购加工经营户收购屠宰未经检疫的淘汰和病死毛兔的违法行为,执法人员立即赶赴现场检查,发现当事人番某的冷库中确实有屠宰加工好但未经检疫的冻兔肉249公斤和120公斤兔皮。同时在     

基质对黑果腺肋花楸幼苗生长的影响

以黑果腺肋花楸(Aronia melanocarpa)为试验材料,选用田园土、泥炭土、珍珠岩和黄沙,采用正交试验设计,研究不同基质配比对黑果腺肋花楸成苗率以及幼苗形态指标和生理指标的影响。结果表明,不同育苗基质对黑果腺肋花楸出苗率、苗高、地径、主根长、侧根数和高径比等形态指标以及根系活力、可溶性糖、淀粉和蛋白质等生理指标的影响差异显著,综合分析各项指标,筛选出适合黑果腺肋花楸容器育苗的栽培基质为田园土(40%)+泥炭土(30%)+黄沙(30%)、田园土(40%)+珍珠岩(30%)+黄沙(30%)、田园土(40%)+泥炭土(30%)+珍珠岩(30%)和田园土(60%)+泥炭土(20%)+黄沙(20%)。