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Barbara Meisel Jeanne Korsman Frederik J. Kloppers Dave K. Berger 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(4):577-583
The aim of our study was to identify the causal agent of grey leaf spot disease of maize in southern Africa. Single-conidial
cultures were recovered from maize leaves with typical disease symptoms sampled from several fields in South Africa, Zambia
and Zimbabwe. Morphology, cultural characteristics, and a PCR-based test using Cercospora zeae-maydis and C. zeina-specific primer sets identified all single-conidial cultures as C. zeina. In addition, sequence alignment of DNA fragments of the internal transcribed spacer region (ITS1, ITS2, and the 5.8S gene)
and elongation factor 1-α grouped all cultures in the same clade as the C. zeina ex-type culture CBS 118820. To by-pass cultivation of the slow-growing fungus, a rapid method to isolate DNA directly from
lesions was successfully applied for PCR identification of C. zeina with species-specific ITS and histone primers. Koch’s postulates were fulfilled for C. zeina by artificially inoculating maize plants in a greenhouse, re-isolating conidia emerging from lesions and verifying pathogen
identity with molecular techniques. These results provide evidence that confirms the presence of C. zeina and absence of C. zeae-maydis in commercial maize plantations in southern Africa. 相似文献
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