Measured and estimated water use and crop coefficients of grapevines trained to overhead trellis systems in California’s San Joaquin Valley

The use of overhead trellis systems for the production of dry-on-vine (DOV) raisins and table grapes in California is expanding. Studies were conducted from 2006 to 2009 using Thompson Seedless grapevines grown in a weighing lysimeter trained to an overhead arbor trellis and farmed as DOV raisins for the first two years and for use as table grapes thereafter. Maximum canopy coverage for the two lysimeter vines across years was in excess of 80 %. Seasonal (15 March–31 October) evapotranspiration for the lysimeter vines (ET_Lys) was 952 mm in 2007 (farmed as DOV raisins) and 943 and 952 mm (when farmed as table grapes). The maximum crop coefficient (K _cLys) across all 4 years ranged from 1.3 to 1.4. These maximum values were similar to those estimated using the relationship where K _c is a function of the amount of shaded area measured beneath the canopy at solar noon (K _c = 0.017 × percent shaded area). Covering the lysimeter’s soil surface with plastic (and then removing it) numerous times during the 2009 growing season (1 June–14 September) reduced ET_Lys from an average of 6.4 to 5.6 mm day^?1 and the K _c from 1.07 to 0.93. A seasonal basal K _c (K _cb) was calculated for grapevines using an overhead trellis system with a 13 % reduction in the K _cLys across the growing season.     

Relationships of spectral traits with yield and nutritional quality of snap beans (Phaseolus vulgaris L.) in dry seasons

Four snap bean varieties (green and yellow-podded) were grown in three water supplies (regularly irrigated, deficit-irrigated and non-irrigated) in order to examine the spectral reflectance at leaf and canopy levels, leaf area index (LAI) and their relationships with the yield and nutritional quality at the University of Debrecen, Debrecen, Hungary from 2011 to 2013. Under non-irrigated condition, the plant height decreased, SPAD values were high but LAI and normalized difference vegetation index (NDVI) were low. The difference in SPAD values was higher between the varieties during flowering under non-irrigated condition and it was more pronounced during pod development using deficit irrigation. In dry years, non-irrigated condition SPAD did not correlate with the yield but during pod development it was correlated with the dry matter content of the pods. The relationship of SPAD with protein and crude fiber content of pods was strongly dependent on the cropping years. Under deficit-irrigated condition, NDVI measured during flowering and pod development correlated closely with the yield where a higher NDVI (0.85) predicted either 6 or 9.5 t ha^?1 of yield depending on the variety. Under this condition Serengeti variety with green pods was prominent based on the studied traits among the varieties.     

Atmospheric Mercury Fluxes in a Southern Boreal Forest and Wetland

Total gaseous mercury (TGM) fluxes from the forest floor and a boreal wetland were measured by a flux chamber technique coupled with an automatic mercury vapour analyser. The fluxes were measured at three sampling sites in southern Finland, 61°14′ N, 25°04′ E in summer 2007, with additionally in situ TGM concentrations in the air at one of the sites and mercury bulk deposition at another. Most of the flux data were collected during the daytime. At one of the sites, diurnal flux behaviour was studied, and a clear cycle with an afternoon maximum and a night minimum was observed. The highest emissions (up to 3.5 ng m⁻² h⁻¹) were observed at the forest floor site having a moss and grass cover. At the wetland and litter-rich forest floor sites, the emissions were below 1 ng m⁻² h⁻¹ and sometimes negative (down to −1.0 ng m⁻² h⁻¹), indicating mercury uptake. The measured average fluxes in August were 0.9 ± 1.1 and 0.2 ± 0.3 ng m⁻² h⁻¹ for the forest floor sites and wetland sites, respectively. The flux data were compared with the mercury bulk deposition, which proved to be of the same magnitude, but opposite in sign. At the mossy forest floor site, the extrapolated TGM emissions were 130% of the Hg deposition in August 2007. Comparison with other studies showed that the fluxes in background areas are relatively uniform, regardless of measurement site location and method used. Airborne TGM remained at the background level during the study, with an average value of 1.3 ± 0.2 ng m⁻³; it frequently showed a diurnal cycle pattern.     

Fasciation in <Emphasis Type="Italic">Crassula argentea</Emphasis>: molecular identification of phytoplasmas and associated antioxidative capacity

The present study reports on phytoplasma induced fasciation in Crassula argintea (Crassulaceae). DNA was extracted from symptomless and fasciated tissues and amplified by nested PCR using universal primers P1/P7 followed by R16F2n/R16R2 produced amplicons of 1.2 Kb. The nucleotide sequence analyses of the amplicons indicated that fasciated plants were infected by phytoplasma. Phylogenetic analysis placed the Crassula fasciation phytoplasmas in 16SrII-D group. Histochemical staining for reactive oxygen species indicated that phytoplasma infected (PI) tissues possess significantly higher levels of hydrogen peroxide (H₂O₂) rather than superoxide (O₂ ^·-) as compared with symptomless tissues. PI tissues were also associated with a significant increase in antioxidant enzyme activities (catalase, peroxidase, polyphenol oxidase, and glutathione reductase) and electrolyte leakage as compared with symptomless tissues.     

Pathogens of bark beetles (Coleoptera: Curculionidae) in Bulgarian forests

The occurrence and prevalence of bark beetle pathogens in forest stands in Bulgaria were investigated in 944 specimens belonging to 21 bark beetle species. Protozoa, microsporidia, fungi and nematodes occurred in 19 of all investigated species. The infections were found in the gut (nematodes, gregarines, microsporidia), gonads (microsporidia) and hemolymph (nematodes) of the infected insects. Protozoan species (Gregarina typographi, Gregarina spp.) were detected in eight bark beetle species. Morphometric data about G. typographi and Gregarina spp. are presented. The prevalence of the gregarines varied between 1.4% and 64.2%. Microsporidia of the genera Nosema and Chytridiopsis were revealed in three bark beetle species. The prevalence of microsporidia ranged between 1.5% and 11.8%. This is the first report of a microsporidium in Taphrorychus villifrons and of gregarines in T. villifrons, Pityogenes bistridentatus, P. conjunctus, and Orthotomicus erosus. The fungus Beauveria bassiana was found in 3.4% of Hylurgops palliatus specimens. Nematodes (in gut and haemolymph) were revealed in 19 bark beetle species and their prevalence varied between 10% and 98.5%.     

STUDIES ON THE MANAGEMENT OF CARDIOPULMONARY EMBARRASSMENT DUE TO THIOPENTONE ANAESTHESIA IN THE EQUINE

Cardio pulmonary embarrassment was induced with thiopentone sodium in 35 healthy adult donkeys divided in to seven groups of five animals. “To effect” anaesthetic dose was (12.72 ± 0.69 mg/kg) which caused transient hypotension, tachycardia and hypoxemia. A stable fall in MAP 1. 6.77 ± 0.287 Kpa (50.78 ± 2.15 mmHg) and a flat EEG were considered to indicate acute circulatory insufficiency due to thiopentone overdose.     

Content of Nutrient and Antinutrient in Edible Flowers of Wild Plants in Mexico

Nutrient and antinutritional/toxic factors present in some edible flowers consumed in Mexico were determined. The edible flowers were: Agave salmiana, Aloe vera, Arbutus xalapensis, Cucurbita pepo (cultivated), Erythrina americana, Erythrina caribaea, Euphorbia radians benth and Yucca filifera. The nutrient content in the flowers studied is similar to that of the edible leaves and flowers studied mainly in Africa. The moisture content of the flowers varied from 860 to 932 g kg⁻¹. Crude protein (CP) was between 113 to 275 g kg⁻¹ DM, crude fiber, 104 to 177 g kg⁻¹ DM and the nitrogen free extract, between 425 to 667 g kg⁻¹ DM. The highest chemical score (CS) was found in E. americana and A. salmiana; in five samples the limiting amino acid was lysine, and in three of them it was tryptophan. Trypsin inhibitors and hemaglutinnins had a very low concentration. Alkaloids were present in both the Erythrina species and the saponins in A. salmiana and Y. filifera. Cyanogenic glucosides were not found in the studied flowers. The traditional process of preparing these specific flowers before consumption is by cooking them and discarding the broth; in this way the toxic substances are diminished or eliminated. These edible flowers from wild plants consumed in local areas of the country play an important role in the diet of the people at least during the short time of the season where they are blooming.     

Effect of tool angles on the chips generated during milling of wood by straight router-bits

 The effect of tool angles on the shapes of chips generated by parallel-to-grain and end-grain milling was explored for China fir and maple under fixed spindle and feed speeds and cutting depth. The milling path was up-milling by straight router-bits with a diameter of 12 mm. The chip shapes could be distinguished as five types: spiral, splinter, flow, thin, and granules or powder. The flow and thin chips were generated most often (on a weight percentage basis) for all tool angles investigated for parallel-to-grain and end-grain milling of China fir and maple. More granule chips were produced with parallel-to-grain milling than with end-grain milling for both woods. The measured chip thickness (t′) was thicker than the calculated thickness (t _max). Thicker and longer maple chips were produced by end-grain milling than by parallel-to-grain milling. The tool geometries of 40°/15° (sharpness of the angle–rake angle), 50°/15°, and 60°/15° for China fir and 40°/25°, 50°/5°, and 60°/5° for maple produced relatively more flow chips with parallel-to-grain milling. Furthermore, the tool geometries of 40°/5°, 50°/15° and 60°/25° produced more flow chips (weight percentage) by end-grain milling of China fir and maple. Received: May 23, 2001 / Accepted: June 28, 2002 Acknowledgment This study was supported by a grant from the National Council of Science, Taiwan (NSC89-2313-B-415-011).     

磷肥诱导下层植被伴生的辐射松苗生长、磷素营养和土壤有效磷变化

通过在温室条件下施加不同剂量的重过磷酸钙(0,50和100mg·kg-1P),对典型水铝英石土壤(Orthic Allophanic Soil)的土壤植物有效磷、磷素营养和伴生有金雀花(Cytisus scoparius)或黑麦草(Lolium multiflorum)的辐射松苗木生长变化情况进行了研究。结果表明,施加磷肥增强了辐射松根际和磷素匮乏土体的有效磷。与辐射松苗伴生的金雀花、黑麦草根际土壤有效磷也增加。辐射松新枝和老枝松针、茎和根系中磷含量随施加重过磷酸钙剂量的增加而增加;但是金雀花、黑麦草对辐射松苗磷素营养的影响依赖于土壤磷状态。未施肥处理下,与金雀花伴生的辐射松新枝和老村松针、茎和根系中磷含量要高于与黑麦草伴生的辐射松苗,而施肥处理下,结果相反。这可能与金雀花生长大量消耗土壤中有效磷有关。因为,在两个施肥剂量(50mg·kg-1P和100mg·kg-1P)上,与金雀花伴生的辐射松根际土壤中Bray-2磷含量始终低于与黑麦草伴生的辐射松根际土壤。此外,在施肥浓度100mg·kg-1下,与金雀花伴生的辐射松苗干特质量低于黑麦草伴生的辐射松。说明在中、高施肥水平,辐射松苗与黑麦草伴生生长好于与金雀花伴...     

The effects of maternal nutrition during the first 50 d of gestation on the location and abundance of hexose and cationic amino acid transporters in beef heifer uteroplacental tissues

We hypothesized that maternal nutrition during the first 50 d of gestation would influence the abundance of hexose transporters, SLC2A1, SLC2A3, and SLC2A5, and cationic amino acid transporters, SLC7A1 and SLC7A2, in heifer uteroplacental tissues. Angus-cross heifers (n = 43) were estrus synchronized, bred via artificial insemination, and assigned at breeding to 1 of 2 dietary intake groups (CON = 100% of requirements to achieve 0.45 kg/d of BW gain or RES = 60% of CON intake) and ovariohysterectomized on day 16, 34, or 50 of gestation (n = 6 to 9/d) in a completely randomized design with a 2 × 3 factorial arrangement of treatments. Uterine cross-sections were collected from the horn ipsilateral to the corpus luteum, fixed in 10% neutral buffered formalin, sectioned at 5 µm, and stained via immunofluorescence for transporters. For each image, areas of fetal membrane (FM; chorioallantois), luminal epithelium (ENDO), superficial glands (SG), deep glands (DG), and myometrium (MYO) were analyzed separately for relative intensity of fluorescence as an indicator of transporter abundance. Analysis of FM was only conducted for days 34 and 50. No transporters in target areas were influenced by a day × treatment interaction (P ≥ 0.06). In ENDO, all transporters were differentially abundant from days 16 to 50 of gestation (P ≤ 0.04), and SLC7A2 was greater (P = 0.05) for RES vs. CON. In SG, SLC7A1 and SLC7A2 were greater (P ≤ 0.04) at day 34 vs. day 16. In DG, SLC2A3 and SLC7A1 were greater (P ≤ 0.05) for CON vs. RES heifers; furthermore, SLC7A1 was greater (P < 0.01) at day 50 vs. days 16 and 34 of gestation. In MYO, SLC7A1 was greater (P < 0.01) for CON vs. RES and was greater (P = 0.02) at days 34 and 50 vs. day 16. There were no differences in FM (P ≥ 0.06). Analysis of all uterine tissues at day 16 determined that SLC2A1, SLC2A3, and SLC7A2 were all differentially abundant across uterine tissue type (P < 0.01), and SLC7A1 was greater (P = 0.02) for CON vs. RES. Analysis of all uteroplacental tissues at days 34 and 50 demonstrated that all transporters differed (P < 0.01) across uteroplacental tissues, and SLC7A1 was greater (P < 0.01) for CON vs. RES. These data are interpreted to imply that transporters are differentially affected by day of gestation, and that hexose and cationic amino acid transporters are differentially abundant across utero-placental tissue types, and that SLC7A1 is responsive to maternal nutritional treatment.