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71.
72.
Maria Podsiedlik Erik H. Hofmeister Tanya Duke-Novakovski 《Canadian journal of veterinary research》2022,86(1):20
The paravertebral brachial plexus block (PVB) provides thoracic limb analgesia. The objective was to describe a blind craniocaudal (CC) approach to the PVB and compare its accuracy, time, and difficulty of performance with a blind dorsoventral (DV) approach. The operator was initially trained by experienced clinicians to perform both approaches on 5 cadavers. Next, a CC or DV approach to the PVB was performed on both thoracic limbs of 20 cadavers (20 for each approach). Methylene blue dye was equally divided into 4 aliquots to stain the ventral branches of the sixth to eighth cervical and first thoracic spinal nerves. Successfully stained (stain ≥ 1 cm) spinal nerves were counted. The time to perform each approach was recorded and ease of performance was scored using a numerical scale (1 “easy” to 4 “difficult”). The phrenic nerve was checked for stain. A Wilcoxon signed-rank test was used to compare approaches. The data are presented as median (interquartile range; minimum to maximum range). The number of stained nerves with the CC approach 3 (1; 2 to 4), was higher than the DV approach 2 (2; 0 to 4) (P = 0.002). The time (in seconds) to perform the CC approach 125 (79; 70 to 194), was not different from the DV approach 142 (54; 101 to 232) (P = 0.084). The CC approach 2 (2; 1 to 4) was easier to perform than the DV approach 3 (1; 2 to 4) (P = 0.024). No phrenic nerve staining was observed with either approach. The CC approach is an alternative to the DV approach for performing the PVB in dogs. 相似文献
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74.
Jorge Dias Rebeca Rueda-Jasso Stephan Panserat Luis E C da Conceição Emidio F Gomes & Maria T Dinis 《Aquaculture Research》2004,35(12):1122-1130
A study was undertaken to determine the effect of various dietary carbohydrate‐to‐lipid ratios on growth performance, whole‐body composition and tissue lipid content in Senegalese sole (Solea senegalensis) juveniles. Data on the dietary regulation of key hepatic enzymes of the lipogenic and glycolytic pathways (glucose‐6‐phosphate dehydrogenase, G6PD; malic enzyme, ME; fatty acid synthetase, FAS; pyruvate kinase, PK and glucokinase, GK) were also generated. Four isonitrogenous (crude protein: 52% dry matter (DM)) diets were formulated to contain one of two lipid levels (11% and 21% DM). Within each dietary lipid level, the nature of the carbohydrate fraction (raw or extruded peas) was varied. Triplicate groups of 54 sole (initial body weight: 23.6±1.2 g) were grown in recirculated seawater over 67 days. Fish were fed using automated feeders. At the end of the study, whole‐body, liver, viscera and muscle samples were withdrawn for analyses. During the experimental period, the mean fish weight about doubled in all treatments. No significant differences were found in growth performance (ranging from 1.1% to 1.4% body weight day?1) among dietary treatments. High‐fat diets increased whole‐body fat content. Similarly, daily fat gain ranged from 0.54 to 0.78 g kg?1 day?1 and highest values were found in fish fed high‐lipid diets. Dietary treatments also affected tissue lipid content (liver, viscera and muscle), with highest values in fish fed high‐fat diets. The nature of dietary carbohydrates had little influence on performance criteria, but affected tissue lipid deposition. The activities of G6PD, ME and FAS were depressed by elevated levels of dietary lipid, confirming the inhibitory effect of dietary fats on lipid biosynthesis. At both dietary lipid levels, ME and FAS activities were little affected by dietary carbohydrate. Activities of PK and GK were not affected by the starch level of the diets. In Senegalese sole juveniles, the lipogenic pathway is more susceptible to modulation by dietary means (particularly through lipid intake) than the glycolytic pathway. 相似文献
75.
Maria Rowena R. Romana-Eguia Minoru Ikeda Zubaida U. Basiao Nobuhiko Taniguchi 《Aquaculture International》2010,18(6):1205-1221
Growth of several genetically improved Nile tilapia (GIFT or Genetically Improved Farmed Tilapia, FaST or Freshwater Aquaculture
Center Selected Tilapia, SEAFDEC-selected) and domesticated red tilapia (BFS or Binangonan Freshwater Station, NIFI-red or
National Inland Fisheries Institute red, HL or Hacienda Luisita) stocks were compared in controlled (tank) and uncontrolled
farm conditions (lake-based cages) with unselected NIFI or Chitralada Nile tilapia as control. Specific growth rate differed
significantly (P = 0.009) in tank-reared Nile tilapia stocks where GIFT grew best at 1.358%/day followed by FaST (1.307%/day), control stock
NIFI (1.257%/day) and SEAFDEC-selected (1.202%/day). Genetic effect explained 84.4% of the variance in growth of Nile tilapia
in tanks. Although Nile tilapia growth in cages followed the same trend where GIFT grew best at 1.570%/day, no significant
stock differences (P = 0.479) were noted. Meanwhile, red tilapia reared in either tanks or cages showed no significant stock differences in terms
of growth. However, survival of the red tilapia stocks in cages differed significantly with HL having the highest percentage
survival at 93.3%. The different growth responses of the Nile tilapia stocks especially under controlled (tank) farm conditions
were largely due to genetic factors (stock differences).Under uncontrolled farm conditions, environmental factors were generally
observed to have also affected the survival and to some extent, the growth of Asian Nile and red tilapia stocks. 相似文献
76.
77.
Herwig Waidbacher David M Liti Maria Fungomeli Raphael K Mbaluka Jonathan M Munguti & Michael Straif 《Aquaculture Research》2006,37(6):594-600
The effects of pond fertilization and feeding rate on growth, economic returns and water quality were investigated to develop a low‐cost cage‐cum‐pond integrated system for production of Oreochromis niloticus (L.). Hand‐sexed male fingerlings averaging 19±0.39 and 32±0.69 g were stocked in cages and open ponds at 150 fish cage?1 and 2 fish m?2 respectively. Fish were cultured for 114 days in five triplicate treatments. Cages were installed into ponds and caged fish were fed a 24% protein diet at 3% (T1) and 6% (T2) body weight day?1 (BWD) without pond fertilization, and 6% BWD with pond fertilization (T3). The open water in the fourth treatment (T4) was not stocked but contained caged fish, which were fed 6% BWD for the first 57 days followed by 3% BWD for the remaining period. Ponds in the control (T5) had no cages and were neither fertilized nor open‐pond fish fed. Feeding rate and pond fertilization significantly (P<0.05) affected fish growth, profitability and water quality among treatments. Fish growth, feed utilization, fish yield, water quality and profits were significantly (P<0.05) better in T3 than the other treatments. It was concluded that fish production and economic returns were optimized at 6% BWD in fertilized ponds. 相似文献
78.
Carlos A Martínez-Palacios Maria Gisela Ríos-Durán Jorge Fonseca Madrigal Mayra Toledo Cuevas Angela Sotelo López & Lindsay G Ross 《Aquaculture Research》2008,39(7):738-747
The Mexican silverside, Menidia estor, is a species with great regional importance and with very high prices in local markets. Unfortunately, due to high fisheries pressure, environmental degradation and pollution, the species has become endangered. Recently, there has been much progress in the biotechnology of this species, aimed at its culture, and the present paper describes the advances in feeding and nutrition of those important fish. M. estor is a stomachless, zooplantophagous fish, which also occasionally feeds on small fish and crustaceans in the adult stage. Studies on the digestive enzymatic activities show high proteolytic capacity and a late or different model of digestive maturation from that described for marine stomach fish. Nutritional studies on M. estor have shown that juveniles have dietary requirements of about 400 g kg?1 protein and 80 mg kg?1 vitamin C. The upper level of dietary carbohydrate for good growth and survival for juveniles is about 150 g kg?1. Based on the high docosahexaenoic acid (DHA) content in flesh when fed on diets low in DHA, it is believed that the species has the capacity to biosynthetize >20‐carbon fatty acids from 18‐carbon fatty acids. The high levels of DHA in flesh makes this fish a very significant potential component of human nutrition. Using these findings the first practical diets for commercial culture of the species have been developed. 相似文献
79.
Martina Caramante Rosa Rao Luigi Maria Monti Giandomenico Corrado 《Scientia Horticulturae》2009,120(4):560-564
‘San Marzano’ (SM) is one of the most widely known tomato (Solanum lycopersicum L.) cultivars, and is a classic example of a local variety with a premium value. Unfortunately, the original cultivated form is underrepresented in the Protected Denomination of Origin (PDO) area because of the incidence of contaminant and phenotypically similar genotypes. Our aim was to examine the ability of three DNA marker systems (minisatellite, cleaved amplified polymorphic sequence (CAPS) and simple sequence repeat (SSR)) to reveal the genetic diversity of tomato accessions that were, based on a morphological analysis, very similar. The data indicate that both minisatellites and SSRs can be used to genetically distinguish the analysed materials. Furthermore, these two marker systems depict relationships consistent with the hierarchal pattern obtained by the morphological data. As locally cultivated tomato accessions are often characterised by some degree of genetic variability, our results will be valuable in facilitating the purification, management and breeding of tomato germplasms. The differences between the marker systems employed are also discussed in relation to their usefulness in the agro-food chain. 相似文献
80.