A light and scanning-electron microscopic study of infection of tomato plants by virulent and avirulent races of Cladosporium fulvum

Infection of tomato plants byCladosporium fulvum Cooke was studied using light and scanning-electron microscopy. Races 1.2.3 and 4 ofCladosporium fulvum were used, whereas tomato cultivars, carrying the Cf2 gene (susceptible to race 1.2.3 and immune to race 4) and the Cf4 gene (immune to race 1.2.3 and susceptible to race 4) served as differentials. No differences were observed in growth between compatible and incompatible combinations during germination, subsequent formation of runner hyphae and stomatal penetration. Runner hyphae did not show directional growth towards stomata. Penetration usually occurred on the third or fourth day after inoculation. In compatible combinations the fungus grew intercellularly, often in close contact with spongy mesophyll cells. Under optimal conditions it did not cause visible damage to plant cells during early stages of infection. Under suboptimal conditions in winter, the host cells often reacted with callose deposition, but growth of the fungus did not appear to be inhibited. Ten to twelve days after inoculation conidiophores emerged through the stomata and produced conidia. In incompatible combinations fungal growth was arrested one to two days after penetration and confined to stomata and surrounding cells. Very soon the host cells, in contact with the fungus, deposited extensive amounts of callose. Later these cells turned brown and collapsed. At the surface of the host cells, contacted by fungal hyphae, abundant extracellular material could be observed by scanning-electron microscopy. Removing the epidermis of leaves before inoculation delayed the resistant response. On stripped leaves the rate of fungal growth was equal for both interactions up to ten days after inoculation, but the incompatible combination lacked sporulation.     

Observation on the remarkable stability of transmissible mink encephalopathy virus.

Transmissible mink encephalopathy (TME) virus retained biological activity after prolonged storage in formalin (about three and a half years) and in paraffin tissue blocks used for histologic preparations (about six and a half years). Thus, TME tissues in formalin and in paraffin blocks should be handled as contaminated materials.     

Variation of canine and feline frontal-plane QRS axes with lead choice and augmentation ratio

The intraindividual variability in mean electric axis referable to choice of ECG leads and augmentation ratio was investigated by means of a digital computer program. Although, in theory, the choice of leads for determining the QRS axis should make no difference, the maximum intraindividual variation in axis angle was about 50 degrees for canine and feline subjects. The angle conputed for the lead combinations I and III and I and aVF will usually fall within 5 to 10 degrees of the mean of all lead combinations, but larger variations are seen at time. The application of an "augmentation ratio" for the augmented unipolar leads was most helpful when it was individually computed. Further methods of lead-vector improvement are discussed.     

Tiering in suspension-feeding communities on soft substrata throughout the phanerozoic

Tiering of benthic marine suspension-feeding communities on soft substrata has varied throughout the Phanerozoic. Epifaunal tiering was most developed during the middle and late Paleozoic and the Triassic to Jurassic, with large-scale reductions in tiering occurring during the Permian-Triassic extinctions and after the Jurassic. Infaunal tiering reached its highest level of organization after the Paleozoic.