Design and fabrication of topologically complex, three-dimensional microstructures

Two concepts for use in the fabrication of three-dimensional (3D) microstructures with complex topologies are described. Both routes begin with a two-dimensional (2D) pattern and transform it into a 3D microstructure. The concepts are illustrated by use of soft lithographic techniques to transfer 2D patterns to cylindrical (pseudo-3D) substrates. Subsequent steps-application of uniaxial strain, connection of patterns on intersecting surfaces-transform these patterns into free-standing, 3D, noncylindrically symmetrical microstructures. Microelectrodeposition provides an additive method that strengthens thin metal designs produced by patterning, welds nonconnected structures, and enables the high-strain deformations required in one method to be carried out successfully.     

Detecting Seasonal Flow Pathways in Road Structures Using Tracer Tests and ERT

Roads and traffic can be a source of water-bound pollutants, which can percolate through the unsaturated zone to groundwater. Deicing salt is widely used on roads in northern Europe during winter and is usually applied at a time when the temperature is below zero and the soil is partly frozen. Understanding the mechanism by which water-bound pollutants such as deicing salt are transferred from roads to groundwater is highly important for groundwater protection, environmental sustainability and road maintenance. Electrical resistivity tomography (ERT) can be used for tracing the infiltration of deicing salt in different seasons, including the frozen period, as a step towards identifying pollutant infiltration pathways. In this study, a tracer-ERT monitoring method and analytical process was developed and evaluated for use in investigating and demonstrating deicing salt infiltration pathways in road structures in different seasons and weather conditions. The method involves using dissolved sodium chloride as a tracer and monitoring its infiltration using a multi-electrode array system. The tracer tests were performed at the same location in different seasons over a 1-year period. The results indicated high seasonal variation in percolation pattern and flow velocity, with large decreases in December (winter), most likely due to preferential flow paths within the road shoulder. These findings can be applied to other water-soluble pollutants that move from the road surface to groundwater.     

Effects of Serum Deprivation and Cycloheximide on Cell Cycle of Low and High Passage Porcine Fetal Fibroblasts

Arrest of cells in G0/G1 cell cycle phase is desired for nuclear transfer procedures. Serum starvation and cell cycle inhibitors are different ways to induce synchronization of the cell cycle. This study investigated the effects of serum starvation and cycloheximide (CHX) on the cell cycle of low (5th) and high (15th) passages fetal porcine fibroblasts. Cell cycle phases were determined using fluorescent activated cell sorting. Fifth passage fibroblast cultures had higher (p < 0.05) proportion of cells in G0/G1 only after 72 h of serum starvation (77.60 ± 0.65) when compared with non‐starved cells (71.44 ± 1.88). Serum starvation for all periods tested induced an increase (p < 0.05) on proportion of cells in G0/G1 on the 15th passage. No significant differences were observed on the 5th passage cultures exposed to CHX, although, on the 15th passage an increase on proportion of cells was observed after all periods of exposure (p < 0.05). These data indicates that high passage cells in vitro are more susceptible to serum starvation and CHX G0/G1 synchronization.     

Pre-parturition staphylococcal mastitis in primiparous replacement goats: persistence over lactation and sources of infection

This investigation reported for the first time the occurrence of intramammary infections caused by Staphylococcus in primiparous replacement goats before parturition and the persistence of clinical Staphylococcus aureus infection during the lactation period. Subclinical infections, mainly caused by coagulase negative staphylococci (CoNS), did not persist during lactation. Genotyping analysis indicated that environment seems to play a moderate role as source of intramammary infections to goats before parturition, but causative agents of mastitis in lactating animals are not genotypically related to environmental staphylococci. The occurrence and persistence of intramammary infections in replacement goats demonstrate the need to consider those animals as potential sources of infections in dairy goat herds.