一起销售劣兽药案的查处与思考

云南省德宏州动物卫生监督所通过抽检发现,辖区内某兽药经营服务部涉嫌销售劣兽药。经该县农业局立案调查,认定当事人违法事实清楚,证据充分,根据《兽药管理条例》规定,依法给予了行政处罚。本文重点介绍了案件来源、调查过程、处理情况等执法内容。分析了案件定性、执法主体适格、自由裁量权应用、保护当事人合法权益等问题,以期为同类案件查处提供参考。     

猪德尔塔冠状病毒荧光定量PCR检测方法的建立

根据GenBank登录的猪德尔塔冠状病毒(PDCoV)毒株序列设计特异性引物,建立了检测PDCoV的实时荧光PCR方法。该方法在标准品浓度2.49×10~8~2.49×10~2 copies/μL范围内线性关系良好;特异性试验显示,其他几种常见猪病病毒未见典型扩增曲线;敏感性试验显示,最低检测下限为24.9 copies/μL;重复性试验显示,批内和批间变异系数小于3%。用该方法检测62份猪腹泻粪便样品,相比常规PCR方法,前者的检出率更高;两种方法检测1 168份进境活猪粪便拭子样品,结果均为阴性。结果表明,本研究所建立的方法适用于国内猪场的PDCoV的检测和进境活猪的监控检测,也可作为PDCoV的诊断及分子流行病学调查的技术手段。     

浙西南山区巨峰葡萄平棚避雨栽培病害发生及防治

总结浙西南山区巨峰葡萄平棚避雨栽培主要病害的发生规律,并提出防治措施。     

TFSQ型高效环保组合式粮食杂质清理筛的研制

通过对振动筛理、垂直风选、旋风分离与沉降系统的研究,研制出集振动筛理、风选、旋风分离沉降、含尘气流循环再利用为一体的TFSQ型高效环保组合式粮食杂质清理筛。经应用测试和专家鉴定:该设备具有清理效率高、清理过程粉尘污染小,使用维护方便、可靠性强等优点,具有广阔的市场推广前景。     

Effect of L-lysine Added in Diluent on the Fresh Semen Quality of Dorper Sheep

In order to explore the effect of different L-lysine levels added in semen dilution on the fresh Dorper sheep quality stored at liquid state,3 health Dorper ram were used to collect semen which were equal-packaged and added to solution containing different levels (0,0.1,0.2,0.3,0.4 and 0.5 g) L-lysine in 120 mL diluent and at 0,6,24,30,48,54,72 and 78 h,the sperm motility,membrane integrity and acrosome integrity were detected.The results showed that the sperm motility in groups added 0,0.1 and 0.2 g L-lysine were higher than other groups,and that in 0.4 and 0.5 g L-lysine groups decreased faster than other groups;The sperm motility in 0.5 g L-lysine group reduced to 0 at 30 h,and it reduced to 0 at 48 h in the group added 0.4 g L-lysine;The effective survival time and survival index in the group added 0.1 g L-lysine was higher than other groups (P< 0.05);The membrane integrity of 0.1 g group was the highest,that had no significant difference with control group from 0 to 54 h (P >0.05),while after 72 h the two groups had significant differences (P< 0.05).The membrane integrity in 0.4 and 0.5 g groups were the worst,and that was significant different with other groups (P< 0.05);The acrosome integrity of 0.1 g group was the highest which had no significant difference with control group (P >0.05),0.4 and 0.5 g groups were the worst,the difference between them was not significant (P >0.05),while was extremely significant difference with other groups except for 0 and 6 h (P< 0.01).The results suggested that dilution added a high concentration of L-lysine could inhibit sperm quality,when the count of L-lysine was more than 0.2 g,sperm quality was significantly decreased,adding 0.1 g L-lysine could improve Dorper sheep fresh sperm quality stored at liquid state.     

TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE2分泌的影响

[目的] 进一步探讨牛磺鹅去氧胆酸（taurochenodeoxycholic acid,TCDCA）在抗炎免疫方面的潜在调节作用。[方法] 以AA大鼠成纤维样滑膜细胞作为研究对象,采用ELISA方法检测TCDCA和IL-1β作用下AA大鼠成纤维样滑膜细胞上清液中PGE₂的含量,分析TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂分泌情况的影响。[结果] TCDCA能够对IL-1β刺激下AA大鼠纤维样滑膜细胞PGE₂的分泌产生下调作用（P<0.05）。[结论] TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂的分泌具有抑制作用,为TCDCA在兽医临床应用提供依据。     

赤霉素调控植物块茎形态建成的研究进展

赤霉素对植物的生长发育具有重要的调控作用。综述了赤霉素调控块茎形态建成的研究进展。块茎形态建成是一个复杂的生理过程,受遗传、坏境等因素共同调控,其中,激素调控发挥重要作用。虽然多种激素参与调控块茎的形态建成,但它们的效果最终均依赖于赤霉素含量。一般认为赤霉素抑制或延缓块茎的形成,但对块茎生长膨大的作用有不同的看法。已明确植物体内赤霉素转导的GA-GID1-DELLA路径,但是否在块茎形态建成过程中发挥作用还不清楚。最后提出块茎形态建成可能分为块茎启动和生长膨大两个不同阶段,分属于两个独立的调控系统,同时存在不同于GA-GID1-DELLA信号通路的赤霉素途径,赤霉素在其中发挥的作用不同,机制也不同。     

春小麦新品种酒春7号选育报告

酒春7号是以自育品系酒0325 F_1为母本,冀89-6091为父本杂交选育而成的春小麦新品种。2012—2013年参加甘肃省(西片)水地组区域试验,2 a平均折合产量7 720.5 kg/hm~2,较对照品种宁春4号增产9.9%。2014年参加甘肃省(西片)水地组生产试验,平均折合产量8 091.3 kg/hm~2,较对照品种宁春4号增产6.8%。籽粒含粗蛋白(干基)124.9 g/kg、湿面筋27.1%(以14%水分计),Zeleny沉淀值28.2 m L。适宜在甘肃酒泉、张掖、武威等生态条件相类似区域种植。     

感应热处理工艺对拖拉机轴类零件扭转强度的影响

对驱动轮工艺轴进行了7种工艺方案的感应热处理表面强化,然后对工艺轴进行扭转破坏试验。通过对驱动轮工艺轴扭转断裂的研究,为驱动轮轴及承受扭力的轴类零件结构设计、工艺流程设计和热处理技术要求提供指导,从而提高其扭转疲劳强度和使用寿命。     

基于TRIZ的方草捆收集车集捆装置设计

针对方草捆收集车集捆装置工作稳定性较差的问题，利用TRIZ系统分析方法及ProInnovator软件对集捆装置进行分析，提出了改进方案，对改进方案进行综合评价，得到了最优方案。