Equine chronic diarrhea: Traditional Chinese veterinary medicine review

Chronic diarrhea is a common problem in equine practice. Herbal medicine has been used for the treatment of gastrointestinal disease in horses for at least one thousand years. In present-day equine practice in China, the most common approach to treat chronic diarrhea is herbal medicine, or a combination of herbal medicine and acupuncture. Three types of chronic diarrhea in horses will be described in detail in term of symptoms, diagnosis and herbal treatments in this paper. Nineteen herbal formulas for treatment of different types of diarrhea will be introduced. Clinical results of each herbal formula will be evaluated. Acupuncture treatment of this problem will also be included.     

Studies on the pathogenicity ofBabesia bovis in water buffaloes after cryopreservation and resuscitation

Tropical Animal Health and Production - Packed erythrocytes infected withBabesia bovis were mixed with an equal volume of 16% dimethyl sulphoxide (DMS0) in Alsever’s solution and dispensed...     

Multiple amino acid substitutions are involved in the adaptation of H9N2 avian influenza virus to mice

To explore adaptation of avian influenza virus to mice we previously performed serial lung-to-lung passages of the influenza A/Chicken/Jiangsu/7/2002 (H9N2) strain, resulting in the isolation of a variant influenza strain lethal for mice. We now report that virulence correlates with improved growth characteristics on mammalian cells and extended tissue tropism in vivo. Sequencing of the complete genomes of the wild-type and mouse-adapted viruses revealed 25 amino acid substitutions. Some were found to reiterate known substitutions in human and swine H9N2 influenza isolates. Functions affected include nuclear localization signals and sites of protein and RNA interaction, while others are known determinants of pathogenicity and host specificity such as the viral polymerase PB2 E627K substitution. These observations suggest that enhanced growth characteristics and modified cell tropism may contribute to increased virulence in mice. We conclude that multiple amino acid substitutions are likely to be involved in the adaptation of H9N2 avian influenza virus to mice.     

甘肃省临洮县及周边县区绵羊肝片吸虫感染情况调查

为掌握甘肃省临洮县及其周边县区绵羊肝片吸虫感染情况,2016—2020年采用局部蠕虫剖检术检查屠宰绵羊18373只,发现绵羊肝片吸虫平均感染率为43.1％,平均感染强度为39.3条.荷虫量1～50条的占感染羊的90.6％,51～100条的占9.3％,101条以上的占0.1％;2018年感染率最高(53.3％),平均感染...     

K_(Ca)3.1通道与血管功能的研究进展

研究表明,在促进血管疾病发展的众多细胞类型中,如T细胞、B细胞、内皮细胞、成纤维细胞、巨噬细胞以及去分化的平滑肌细胞,KCa3.1都起到了一定的作用。生理上,KCa3.1已被证明在乙酰胆碱及内皮源性超极化因子(EDHF)介导下发生超极化,从而发挥控制血压的作用。病理上,KCa3.1异常则可导致血管平滑肌细胞增殖迁移。因此,从KCa3.1在内皮细胞及平滑肌细胞两方面对其作用作一总结,并对KCa3.1通道的研究进展进行综述。     

东北虎主要消化腺的组织学研究

通过光镜对东北虎主要几种消化腺的H.E染色石蜡切片进行观察,结果表明,东北虎肝小叶间的结缔组织不发达,小叶的界限不清晰,肝细胞体积较大,核位于细胞一侧,肝细胞多呈索状排列,窦状隙不明显;胰腺外分泌部由腺泡和导管组成,腺泡形状不一,由单层锥状腺上皮细胞组成,胰岛是一些大小不等的细胞团和细胞索,分散于外分泌部之间,胞质染色较外分泌部浅;腮腺为纯浆液腺泡,胞质着色呈嗜酸性,颌下腺是以黏液性细胞为主,含少量的浆液性细胞半月。     

用重组核衣壳蛋白ELISA检测PRRSV抗体的研究

ＰＲＲＳＶ重组Ｎ蛋白ＥＬＩＳＡ检测人工感染猪血清７５份,与ＩＤＥＸＸ公司ＥＬＩＳＡ试剂盒及ＩＦＡ的符合率分别为９７．３％和１００％。检测疫苗免疫猪血清,阳性率１００％。在免疫后６ｄ就能检出抗体,比ＩＦＡ敏感。检测８５份我国田间猪血清,阳性率１８．８％。     

湘西黄牛肉质特性研究

为研究我国南方优良地方品种湘西黄牛的肉质特性,选用8头湘西黄牛青年公牛,对肉质中的常规营养成分、氨基酸和脂肪酸进行了测定.结果表明:鲜肉中脂肪含量为4.80％,蛋白质含量为23.21％;饱和脂肪酸(SFA)含量为48.43％,不饱和脂肪酸含量(UFA)为41.00％,不饱和脂肪酸在总脂肪酸(TFA)的比例为45.85％...     

畜禽养殖场环境微生物致病性和耐药性调查研究

为了解掌握养殖场中环境微生物对养殖业和公共卫生造成的影响,初步对我国养殖场环境微生物进行了研究。对吉林、湖北等省份的部分养猪场和养鸡场环境采样,并进行大肠杆菌和沙门氏菌分离鉴定,同时对从鸡舍和猪舍内分离大肠杆菌和沙门氏菌进行动物致病性实验及对13种抗生素的耐药性实验。实验结果表明,所有实验的沙门氏菌和90.7%的大肠杆菌菌株对小白鼠有致病性;所有分离菌株对磺胺类药物的耐药性非常严重,其次是对四环素,对其他药物也有不同程度的耐药性。     

熊蜂滞育及调控机制研究进展

滞育是绝大多数昆虫为了在不利于自身生存的极端自然环境中存活下来而进行的一种生理过程,是昆虫与环境长期协同进化的结果。熊蜂滞育过程属于成虫生殖滞育,其滞育进程可能受保幼激素水平、热激蛋白表达以及物质代谢等方面的影响,但其具体的滞育调节机制尚未清楚。本文从昆虫滞育的相关假说、熊蜂滞育进程以及熊蜂滞育机制调控进展等方面进行阐述,以期为后续熊蜂工厂化繁育中滞育技术的突破奠定基础。