Sedative effects of intramuscular administration of a low dose of romifidine in dogs

OBJECTIVE: To evaluate sedative effects of IM administration of a low dose of romifidine in dogs. ANIMALS: 13 healthy adult Beagles. PROCEDURE: Physiologic saline solution (0.2 ml), 0.1 % romifidine (10, 20, or 40 microg/kg), or 10% xylazine (1 mg/kg) was given IM in a crossover study design. Heart rate, respiratory rate, rectal temperature, hemoglobin saturation, and scores for sedation, muscle relaxation, posture, auditory response, and positioning response were recorded before and at regular intervals for up to 240 minutes after drug administration. RESULTS: Scores for sedation, muscle relaxation, posture, auditory response, and positioning response increased in a dose-dependent manner after romifidine administration. Sedation induced by the highest dose of romifidine (40 microg/kg) was comparable to that induced by xylazine (1 mg/kg). Heart rate, respiratory rate, and rectal temperature decreased in a dose-dependent manner after romifidine administration, but hemoglobin saturation did not change. CONCLUSIONS AND CLINICAL IMPLICATIONS: Romifidine (10, 20, or 40 microg/kg, IM) is an effective sedative in dogs, but causes a decrease in heart rate, respiratory rate, and rectal temperature.     

Canadian veterinarians' use of analgesics in cattle, pigs, and horses in 2004 and 2005

Anecdotal evidence suggests that many veterinarians may not use analgesics in livestock for routine surgical procedures or painful disease states. To investigate this, we conducted a national mail survey of a random sample of 1431 Canadian veterinarians (response rate, 50.1%). Questions primarily concerned veterinarians' analgesic usage for common surgeries and medical conditions in beef and dairy cattle, pigs, and horses, and attitudes toward pain management. More than 90% of veterinarians used analgesic drugs for equine surgeries, for cesarean section in sows and cows, and for bovine claw amputation and omentopexy. However, in these and other categories, the analgesics used were often inadequate, and many veterinarians did not give analgesics to young animals. When castrated, < 0.001% of piglets received analgesia, compared with 6.9% of beef calves and 18.7% of dairy calves < or = 6 mo of age, 19.9% of beef calves and 33.2% of dairy calves > 6 mo of age, and 95.8% of horses. Respondents largely agreed that there are no long-acting, cost-effective analgesics available for use in livestock (median rating 8/10; interquartile range 4-9), and that the long or unknown withdrawal periods of some drugs outweighed the benefits of using them (median rating 7/10; interquartile range 4-9). The results indicate an urgent need for veterinarians to manage pain in livestock better. Continuing education would help, as would an increase in the number of approved, cost-effective analgesic drugs with known withdrawal periods.     

Tillage, crop residue and N fertilizer effects on crop yield, nutrient uptake, soil quality and nitrous oxide gas emissions in a second 4-yr rotation cycle

An 8-yr (1998–2005) field experiment was conducted on a Gray Luvisol (Boralf) soil near Star City, Saskatchewan, Canada, to determine the effects of tillage (no-tillage – NT and conventional tillage – CT), straw management (straw retained – R and straw not retained – NR) and N fertilizer (0, 40, 80 and 120 kg N ha⁻¹, except no N to pea (Pisum sativum L.) phase of the rotation) on seed and straw yield, mass of N and C in crop, organic C and N, inorganic N and aggregation in soil, and nitrous oxide (N₂O) emissions for a second 4-yr rotation cycle (2002–2005). The plots were seeded to barley (Hordeum vulgare L.) in 2002, pea in 2003, wheat (Triticum aestivum L.) in 2004 and canola (Brassica napus L.) in 2005. Seed, straw and chaff yield, root mass, and mass of N and C in crop increased with increasing N rate for barley in 2002, wheat in 2004 and canola in 2005. No-till produced greater seed (by 51%), straw (23%) and chaff (13%) yield of barley than CT in 2002, but seed yield for wheat in 2004, and seed and straw yield for canola in 2005 were greater under CT than NT. Straw retention increased seed (by 62%), straw (by 43%) and chaff (by 12%) yield, and root mass (by 11%) compared to straw removal for barley in 2002, wheat in 2004, and seed and straw yield for pea in 2003. No-till resulted in greater mass of N in seed, and mass of C in seed, straw, chaff and root than CT for barley in 2002, but mass of N and C were greater under CT than NT for wheat in 2004 and for canola in 2005 in many cases. Straw retention had greater mass of N and C in seed, straw, chaff and root in most cases compared to straw removal for barley in 2002, pea in 2003 and wheat in 2004. Soil moisture content in spring was higher under NT than CT and with R than NR in the 0–15 cm depth, with the highest moisture content in the NT + R treatment in many cases. After eight crop seasons, tillage and straw management had no effect on total organic C (TOC) and N (TON) in the 0–15 cm soil, but light fraction organic C (LFOC) and N (LFON), respectively, were greater by 1.275 Mg C ha⁻¹ and 0.031 Mg N ha⁻¹ with R than NR, and also greater by 0.563 Mg C ha⁻¹ and 0.044 Mg N ha⁻¹ under NT than CT. There was no effect of tillage, straw and N fertilization on the NH₄-N in soil in most cases, but R treatment had higher NO₃-N concentration in the 0–15 cm soil than NR. The NO₃-N concentration in the 0–15, 15–30 and 30–60 cm soil layers increased (though small) with increasing N rate. The R treatment had 6.7% lower proportion of fine (<0.83 mm diameter) and 8.6% greater proportion of large (>38.0 mm) dry aggregates, and 4.5 mm larger mean weight diameter (MWD) compared to NR treatment. This suggests a lower potential for soil erosion when crop residues are retained. There was no beneficial effect of elimination of tillage on soil aggregation. The amount of N lost as N₂O was higher from N-fertilized (580 g N ha⁻¹) than from zero-N (155 g N ha⁻¹) plots, and also higher in CT (398 g N ha⁻¹) than NT (340 g N ha⁻¹) in some cases. In conclusion, retaining crop residues along with no-tillage improved some soil properties and may also be better for the environment and the sustainability of high crop production. Nitrogen fertilization improved crop production and some soil quality attributes, but also increased the potential for NO₃-N leaching and N₂O-N emissions, especially when applied in excess of crop requirements.     

Identification and quantification of subsidies relevant to the production of local and imported pig breeds in Vietnam

Livestock diversity contributes in many ways to human survival and well-being, while its loss reduces options for attaining sustainable agriculture and universal food security. The current rapid rate of loss of this diversity is the result of a number of underlying factors. While in some cases changes in production systems and consumer preferences reflect the natural evolution of developing economies and markets, in other cases production systems, breed choice and consumer preferences have been distorted by local, national and international policy. In the context of a widespread threat to local pig breeds in Vietnam, this paper identifies and quantifies the level of agricultural subsidies that are currently contributing to this process of breed substitution. Producer subsidies—which tend to improve the competitiveness of imported breeds and their crosses over local breeds—are shown to be considerable, and mitigating measures are now urgently needed to avoid an irreversible loss of livestock diversity.     

Pharmacologic-endocrinological findings in animal experiments with TURISYNCHRON and SUISYNCHROM. 2. Toxicologic findings

Acute, subacute and chronic toxicity of TURISYNCHRON and its zinc complex (SUISYNCHRON) was tested in mice, rats and dogs. The acute toxicity of SUISYNCHRON was lower than that of TURISYNCHRON in mice and rats. Ulcerative lesions in the duodenum produced by high doses of SUISYNCHRON were quantitatively less pronounced than those produced by similar doses of TURISYNCHRON. Subacute toxicity testing in rats showed that neither preparation had any toxic effect on haematological, clinical chemical or histological criteria in the dosages selected. Chronic toxicity testing of TURISYNCHRON in dogs did not reveal any evidence of toxic damage.     

Nutrition security, livelihoods and HIV/AIDS: implications for research among farm worker households in South Africa

OBJECTIVE: Previous research on the health transition and nutrition security in black South African households revealed the need for further research among farm workers who belong to the poorest population categories. This article gives an overview of the link between nutrition security, livelihoods and HIV/AIDS in South Africa, drawing conclusions for research among farm worker households. DESIGN AND SETTING: A literature review, observations on farms and interviews with farmers and key informants were carried out in 2003 and 2004. Peer examination was done with South African and German researchers from the natural and social sciences. RESULTS: Farm workers face poverty and nutrition insecurity and continue to be a tragically underserved population group, also in terms of research. There is furthermore a lack of in-depth research on underlying causes for nutrition security in South Africa and on the link with livelihoods, poverty and HIV/AIDS. Micro-social qualitative research from the household and gender perspective is required, as valid data on households, their internal dynamics and therefore the reflection of social realities are missing. A multidisciplinary research approach based on a new conceptual framework was developed to address the situation of farm worker households. CONCLUSION: The outlined research contributes to existing programmes on farms in the North West Province, with the findings being valid also for other parts of southern Africa facing poverty, nutrition insecurity and HIV/AIDS.     

Carbohydrate epitopes are immunodominant at the surface of infectious Neoparamoeba spp

Amoebic gill disease, the main disease of concern to the salmon industry in Tasmania, is caused by the amoeba, Neoparamoeba spp. Experimental infection can only be induced by exposure to wild-type (WT) parasites isolated from the gills of infected fish, as cultured amoebae are non-infective. To characterize the surface antigens of WT parasites, we produced monoclonal antibodies (mAbs) using subtractive immunization. Mice inoculated with non-infective parasites were treated with cyclophosphamide, to deplete reactive lymphocytes, and then immunized with different antigen preparations from infective parasites. When whole parasites were used for boosting, the percentage of WT unique mAbs was very high (86%) as was the percentage of mAbs specific for carbohydrate epitopes (89%). When deglycosylated membranes were used, the numbers of mAbs specific for non-carbohydrate epitopes did not increase, but the total number of WT unique mAbs was reduced (86-40%). Using an untreated membrane preparation, the total number of mAbs to surface molecules was very high, but all recognized carbohydrate epitopes. The total number of mAbs recognizing carbohydrate epitopes on the surface of the WT parasites was 97%, suggesting that the dominant epitopes on the surface molecules unique to WT parasites are carbohydrate in nature.     

Local and regional anesthesia.

Local anesthetics have the unique ability to produce complete blockade of sensory nerve fibers and prevent or pre-empt the development of secondary (central) sensitization to pain. For this reason, local and regional anesthetic techniques are often used with opioids, alpha 2-receptor agonists, dissociatives, and anti-inflammatory drugs as part of a multimodal strategy to manage pain. Lidocaine and bupivacaine are the local anesthetics used most commonly in dogs and cats. Lidocaine has a fast onset (10-15 min) and an intermediate duration of action (60-120 min), and is used for short diagnostic and surgical procedures. Bupivacaine has a slow onset (20-30 min) and a long duration of action (240-360 min), and is used to control pain both preoperatively and postoperatively. Local anesthetics are relatively safe if they are administered correctly. Administration of an excessive dose and accidental intravenous administration are probably the most common causes of systemic toxicity in small animals. Doses of local anesthetics, especially those for cats and small dogs, should always be calculated carefully. In many animals, the most simple and elegant way to control pain perioperatively is to perform a local or regional anesthetic block. Veterinarians should not hesitate to incorporate local and regional anesthetic techniques into their pain management strategies for dogs and cats.     

Factors affecting Canadian veterinarians' use of analgesics when dehorning beef and dairy calves

Data collected through a national, randomized mail survey (response rate 50%) were used to identify reasons why veterinarians were likely (i) to use analgesic drugs when dehorning calves, and (ii) to perceive dehorning without analgesia as very painful. Logistic regression analysis indicated that veterinarians were more likely to be analgesic users the more they perceived that dehorning without analgesia was painful (OR = 1.7, P < 0.001). Other positive influences were if the veterinarian worked in British Columbia or Alberta (OR = 5.9, P = 0.005), and if they were primarily in dairy practice (OR = 3.7, P = 0.012) rather than beef practice. This effect of dairy practice was negated if the veterinarian also perceived that owners were unwilling to pay for analgesia (interaction term: OR = 0.25, P = 0.038). Veterinarians were also less likely to perceive dehorning without analgesia as very painful if they perceived that owners were unwilling to pay (OR = 0.58, P = 0.029). However, this effect on pain perception was offset by concern for personal safety (OR = 2.7, P = 0.015). The results are consistent with the relatively high level of outreach about animal welfare among farmers and veterinarians in the western provinces. The results confirm that many veterinarians' approach to pain management for dehorning is influenced considerably by concern about cost. However, pain management for dehorning is not expensive and there is unequivocal evidence that dehorning calves without pain management causes significant distress. Continuing education of veterinarians should help to increase analgesic usage.     

Community engagement in biobanking: Experiences from the eMERGE Network

Advances in genomic technologies and the promise of "personalised medicine" have spurred the interest of researchers, healthcare systems, and the general public. However, the success of population-based genetic studies depends on the willingness of large numbers of individuals and diverse communities to grant researchers access to detailed medical and genetic information. Certain features of this kind of research - such as the establishment of biobanks and prospective data collection from participants' electronic medical records - make the potential risks and benefits to participants difficult to specify in advance. Therefore, community input into biobank processes is essential. In this report, we describe community engagement efforts undertaken by six United States biobanks, various outcomes from these engagements, and lessons learned. Our aim is to provide useful insights and potential strategies for the various disciplines that work with communities involved in biobank-based genomic research.