Effects of stable dust on farm animals-a review

The relationship between air quality-its dust content-and respiratory diseases of animals in the food industry and otherwise in examined. The correlation between dust content and incidence of disease in farm animals is also discussed. Lesions caused either directly or indirectly by dusttransported microorganisms are reviewd. Effects of dust on animal performance are included.This review is an attempt to compile available information on the natural occurrence of stable dust and the possibilities for its measurement and control.     

Soluble expression of a CXCL10-loop3-EGF fusion protein and its anti-tumor activity

AIM: To evaluate the implication of CXCL10-loop3-EGF fusion protein for the activities of targeting tumor and anti-angiopoiesis. METHODS: RT-PCR was preformed to amplify CXCL10 coding sequence from PBMC activated by IFN-γ. CXCL10-loop3-EGF fusion gene, which was conducted by Over-Lap Extention PCR, was hinged up with plasmid pTG19-T, transfected to E. coli DH5α and processed positive colony selection. After ligated with plasmid pET32a(+), recombinant CXCL10-loop3-EGF fusion gene was then transfected to E. coli Origami B (DE3) and induced to express its coding fusion protein his-CXCL10-loop3-EGF. The recombinant fusion protein CXCL10-loop3 -EGF was purified by His-bind affinity chromatograph, enterokinase cleavage, ultrafiltration and dislysis. The transwell chemotatic test and HUVEC angiopoiesis inhibition test were performed to determine the anti-tumor responses and anti-angiopoiesis activity of CXCL10-loop3-EGF fusion protein. RESULTS: CXCL10-loop3-EGF fusion protein was successfully constructed and confirmed by SDS-PAGE analysis and Western blotting. Significant PBMC chematatic activity and HUVEC anti-angiopoiesis activity were observed. CONCLUSION: CXCL10-loop3-EGF fusion protein, which has perfect anti-tumor activity, is successfully constructed.     

银染mRNA差异显示方法在致突变实验中的初步应用

为建立在药物致突变研究中应用银染 m RNA差异显示的方法 ,试验提取未经过 /经过环磷酰胺处理的 Balb/c小鼠肝组织的总 RNA ,并以此为模板 ,采用 d T1 2 CG、d T1 2 AG、d T1 2 GG为锚定引物 ,通过反转录、差异显示 PCR反应 ,以 6 %变性聚丙烯酰胺凝胶电泳分离差异条带 ,回收后将其再扩增。结果表明 ,RNA投入量为 3μg、镁离子浓度为 1.5～ 2 .0 mm ol/L、退火温度为 4 0℃时 ,扩增的片段条带清晰、背景低 ,差异条带明显 ,再扩增条带单一。银染 m RNA差异显示技术可成功应用于药物致突变的研究。     

Islet cells modified with PD-L1-GPI molecule inhibit attack of reactive T cells

AIM: To investigate the immune depressive effect on the reactive T cells and to explore the immunologic injury mechanism of beta cells of islet in type 1 diabetes mellitus (DM-1). METHODS: pAd5/ PD-L1-GPI adenovirus vector with target gene was constructed and transfected into NIT cells which are known as a mouse insuloma cell line. The highly expressed membrane protein of PD-L1-GPI was confirmed by Western blotting. The peripheral blood non-adherence lymph leukocytes and target cells were cultured to detect lymph leukocyte proliferation and the T cell function. The level of IL-2, TNF-α and IFN-γ were detected in the cell culture fluid. RESULTS: Compared with the control group, the NIT cells modified with PD-L1-GPI inhibited the sensitized lymph leukocyte proliferation effectively and down-regulated the level of some cytokine secretions such as IL-2, IFN-γ and TNF-α (P<0.05). CONCLUSION: The islet cells expressed the PD-L1 gene inhibit the reactive responsive T cells, block up the cytotoxic effect of autoimmunity T cells, and induce the immunotolerance, which would be a value direction of the therapy of DM.     

菜豆抗草甘膦基因SSR分子标记的研究

利用抗草甘膦亲本89-05-3与敏感草甘膦亲本89-13杂交后获得F2代群体,采用BSA法,以上述F2代为定位群体,利用抗、感亲本和抗、感池筛选10对SSR引物.结果袁明:3对引物BM143、BM156、BM164均能在抗、感池间扩增出多态性条带,用Mapmaker 3.0软件作图,将该抗草甘膦基因初步定位在引物BM156和BM164之间,遗传距离分别为3.3 cM和4.4 cM,并暂命名为EPSPS2.该研究结果为莱豆抗性育种以及抗草甘膦基因的精细定位和图位克隆奠定了基础.     

微囊化沙门菌SP4噬菌体的制备及其缓释特性分析

通过采用阳离子醚化淀粉/海藻酸钠/黄原胶/纳米TiO₂/壳寡糖为原料制备微囊化SP4噬菌体,为控制沙门菌感染提供生物制剂。选用沙门菌SP4噬菌体,采用液滴法来制备微囊化SP4噬菌体,并对其效价、阳离子醚化淀粉浓度、海藻酸钠浓度、黄原胶浓度、壳寡糖浓度、pH稳定性、热稳定性、模拟胃液稳定性、模拟肠液释放行为以及保存稳定性进行分析。结果显示,当阳离子醚化淀粉浓度为2.4%、海藻酸钠浓度为2%、黄原胶浓度为1%、壳寡糖浓度为0.6%时,微囊化SP4噬菌体微球外形规则,包封率为97.5%,在pH5.0~8.0、温度10~30℃、模拟胃液pH2.0、0~30 min和pH3.0、0~150 min噬菌体保持较高的生物学活性,模拟肠液中4 h,噬菌体完全释放,4℃环境下保存6周噬菌体效价无明显降低。综上表明,微囊化沙门菌SP4噬菌体显著提高了噬菌体的稳定性和缓释性能,其耐酸特性利于抵御胃酸的分解,具有应用于生物防治的潜力,为进一步采用多因素试验制备微囊化沙门菌SP4噬菌体奠定了基础。     

河南省夏播青贮玉米品种筛选与综合评价

为筛选出适合河南地区种植的优质高产青贮玉米(Zea mays)品种,本研究在河南原阳,选用20个玉米品种作为试验材料,对其干物质生物产量、品质性状(粗蛋白、淀粉、中性洗涤纤维、酸性洗涤纤维、相对饲用价值)、抗逆性(倒伏率、倒折率、茎腐病)和农艺性状(株高、穗位、青贮生育期)等指标进行比较分析.结果表明:1)渝青386、渝青506、北农青贮368、渝青玉3号、新科910、雅玉04889、先玉1658、郑青贮1号、九新631、北农青贮3740和郑青贮2号11个品种干物质生物产量均高于对照雅玉青贮8号,其中先玉1658和九新631籽粒产量均高于郑单958.2)品质指标在20个品种之间差异显著(P＜0.05),通过品质指标聚类分析获得两大类群,其中第Ⅰ类群玉米品种品质较高,除永优1573外其余品种品质等级均为特级或者一级,且该类群玉米品种中性洗涤纤维(neutral detergent fiber,NDF)和酸性洗涤纤维(acid detergent fiber,ADF)含量较低,淀粉含量和相对饲用价值较高.3)渝青玉3号、渝青386、宁禾0709、渝青506、曲辰19、雅玉04889、大京九26和永优1573 8个品种抗倒性较差.从干物质生物产量、品质、抗逆性等综合考虑,北农青贮368、新科910、先玉1658、郑青贮1号、九新631、北农青贮3740、郑青贮2号7个品种表现最佳,适宜作为青贮玉米品种在河南省黄淮海畜牧产业区推广种植.     

不同前处理对测定甜高粱茎秆汁液3种糖含量的影响

茎秆含糖量是甜高粱（Sorghum dochna）重要的农艺性状,其可溶性糖的组分与含量的测定,是甜高粱QTL定位和性状改良的前提。本研究以粒用高粱的‘忻粱52’与甜高粱‘W452’重组自交系F8代255个群体为材料,采用改良蒽酮比色法来测定蔗糖、果糖和葡萄糖含量。结果表明：可溶性总糖的最佳显色时间为10 min,显色温度为100℃;测定蔗糖的最佳处理条件为1 mol·L^-1的KOH溶液处理10 min;果糖测定的最佳显色温度为50℃,显色时间为3 min。优化过后的蒽酮比色法能测得较稳定的数值,精密度和稳定性较好,可用于测定甜高粱汁液的糖含量。重组自交系后代糖锤度与可溶性糖总量之间呈极显著正相关关系（P <0.01）,相关系数为0.885,线性回归方程为y=37.082×可溶性糖总量+3.990。     

褐色凝固型酸奶的制备工艺研究

褐色酸奶是以牛乳和葡萄糖为主要原料,通过加热产生美拉德反应,对褐变后的牛乳进行接种和发酵,得到褐色酸奶制品。本研究以感官得分和乳清析出率为指标,通过单因素试验及正交试验,研究并确定了褐色酸奶的加工工艺参数。试验结果表明,褐色酸奶的最佳加工工艺参数为葡萄糖添加量6%,褐变温度95 ℃,褐变时间5 h,发酵剂接种量5%,果胶添加量0.2%,发酵时间6 h,发酵温度42 ℃。在此条件下得到的褐色酸奶感官评定分数为93分,乳清析出率为5.3%。     

NaOH溶液脱绒对白羊草种子活力的影响

[目的] 探究NaOH溶液脱绒处理对白羊草（Bothriochloa ischaemum）种子活力的影响,为机械化播种提供便利条件。[方法] 分别利用浓度为10%、20%、30%、40%、50%（W/V）的NaOH溶液对白羊草种子进行脱绒处理0（CK）、1、5、10、15 min,分析其对发芽率（Gp）、发芽指数（Gi）、平均发芽时间（MGT）、幼苗活力指数（SVI）的影响,筛选白羊草种子NaOH溶液脱绒的最佳条件。[结果] NaOH溶液浓度及处理时间均对白羊草种子活力具有极显著（P<0.01）影响。相同处理时间下,白羊草种子脱绒1~10 min后Gp、Gi随NaOH溶液浓度的增加而增加,脱绒1 min和10 min后SVI随着NaOH溶液浓度的增加而增加,脱绒5 min后SVI随NaOH溶液浓度的增加呈先降低后升高的趋势,脱绒15 min时Gp、Gi和SVI随NaOH溶液浓度的增加呈先升后降的趋势;在20%~50%的NaOH溶液浓度下,脱绒后白羊草种子Gp、Gi和SVI随处理时间的增加呈先升后降的趋势。经NaOH溶液脱绒处理后白羊草种子的MGT均高于CK。[结论] 适宜浓度的NaOH溶液浸种能有效脱去白羊草种子表面的绒毛,提高白羊草种子的活力。浓度为50%的NaOH溶液浸种5 min对白羊草种子活力的促进效果最好。