水分胁迫与小麦产量性状及籽粒容重的关联度研究

本研究利用相关性分析及灰色关联度分析法,对10个小麦新品种的产量、容重等7个主要性状进行了分析,结果表明：相关性分析结果显示小麦产量性状及容重受水分的影响不同的性状表现不同幅度的减小,灰色关联度结果表明水旱地条件下各性状对单株产量及容重的关联度大小并不一致,各性状对单株产量及容重的关联度大小不同。在沧州地区选择小麦品种时品种的分蘖力和成穗率是首要考虑的条件,穗粒数也要兼顾考虑,小麦籽粒产量及小麦籽粒容重是比较复杂的综合性状,由许多因素相互结合、互相作用。因此,应用灰色关联度分析方法评价性状间的影响程度是客观可行的,可用于今后的育种实践中。     

Dissecting the genetic basis of maize deep-sowing tolerance by combining association mapping and gene expression analysis

Deep-sowing is an important method for avoiding drought stress in crop species,including maize.Identifying candidate genes is the groundwork for investigating the molecular mechanism underlying maize deep-sowing tolerance.This study evaluated four traits(mesocotyl length at 10 and 20 cm planting depths and seedling emergence rate on days 6 and 12) related to deep-sowing tolerance using a large maize population containing 386 inbred lines genotyped with 0.5 million high-quality single nucleotide ...     

杏鲍菇栽培技术研究

杏鲍菇 (Ｐｌｅｕｒｏｔｕｓｅｒｙｎｇｉｉ)是一种品质优良的大型肉质伞菌 ,子实体单生或群生 ,菌肉厚 ,开伞慢 ,柄组织细密结实 ,雪白粗长 ,孢子少 ,保鲜期长、不同于一般平菇 ,味道鲜美 ,被称为“平菇王” ,寡糖含量丰富 ,口感极佳 ,有整肠美容效果 ,市场前景看好。我所在延     

Alteration of apoptotic susceptibility and bcl-2 gene in malignant transformation of human bronchial epithelial cells

AIM: To demonstrate the susceptibility of cell apoptosis varies during the progress of cell malignant transformation from human being in vitro. METHODS: A SV₄₀T-transfected human bronchial epithelial immortalized cell line (called M) was selected in this work, which has acquired some characteristics of malignant transformation at the later passage. The alterations of apoptosis and bcl-2, P53 genes between early and later passage of M cells were investigated by means of TDT labeling in situ, chromosome FISH, RNA and protein testing, etc. RESULTS: Incidence of apoptosis induced by cis-platin was significantly lower in later than in early passages of M. Levels of bcl-2 mRNA and protein in later passages were higher than early passages of M, and overexpression of bcl-2 was accumulated following the development of cellular malignancy. P53 protein level was as high in early as in later passages. CONCLUSION: Overexpression of bcl-2 decreases the cellular sensitivity to apoptotic inductors plays an important role during progress of carcinogenesis in human bronchial epithelial cancers. The inactivation of P53 protein in the SV₄₀-T transfected M cell line may be one of reasons of bcl-2 overexpression, but not associated with the accumulation of bcl-2 expressed level during cell transformation.     

Effect of dimethyl sulfoxide on endotoxin -induced fever in rabbits

AIM:To investigate the effect of dimethyl sulfoxide on endotoxin -induced fever in rabbits. METHODS:Test substances were administered intravenously into a marginal ear vein. Temperature responses were measured using rectal thermistor probes. RESULTS:Endotoxin (0.4 μg/kg) stimulated a biphasic rise in colonic temperature. Pretreatment with dimethyl sulfoxide significantly attenuated the biphasic febrile response to endotoxin (0.4 μg/kg) of conscious rabbits in a dose-dependent manner. Rabbits treated with dimethyl sulfoxide 10 min after injection of endotoxin developed fever, which was significantly milder compared with rabbits treated with an equal volume of normal saline. No significant differences were found between thermal response index of rabbits injected with dimethyl sulfoxide and those with normal saline. CONCLUSION:Dimethyl sulfoxide inhibit endotoxin-induced fever in rabbits.     

Effects of oxidative stress and inflammatory response on kidney injury in mice with hyperthyroidism

AIM To explore the effects of oxidative stress and inflammatory response on kidney injury induced by hyperthyroidism in mice. METHODS Forty male Kunming mice were randomly divided into control group （n=20） and L-thyroxine （T4） group （n=20）. The mice in T4 group were intraperitoneally injected with T4 diluent at a dose of 1 mg/kg to induce hyperthyroidism, and those in control group were injected with normal saline of the same volume. After 7 weeks, the mice were weighed and dissected, the kidneys were removed and weighed, and the length of tibia was also measured. The activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in the kidney tissues were detected. The pathological changes of the kidney tissues were observed by HE staining. The levels of 4-hydroxynonenal （4-HNE）-modified proteins, interleukin-1 receptor-associated kinase 1 （IRAK1） and tumor necrosis factor receptor-related factor 6 （TRAF6） were determined by Western blot and immunohistochemistry. RESULTS Compared with control group, the body weight of the mice was decreased, while the kidney size and weight were increased significantly in T4 group. In addition, the ratios of kidney weight/body weight and kidney weight/tibia length were also increased （P<0.05）. In T4 group, the renal tubules were enlarged, and the epithelial cells of renal tubules were swollen and exfoliated, with vacuolar degeneration. Furthermore, reduced SOD activity, and increased MDA content and 4-HNE-modified proteins were found in T4 group, all of which were related to oxidative stress （P<0.05）. The levels of inflammation-related proteins IRAK1 and TRAF6 were significantly increased in T4 group （P<0.05）. CONCLUSION Excessive T4 may lead to kidney hypertrophy and injury in mice, and the mechanism may be related to oxidative stress and inflammatory response.     

播期播量耦合对小麦越冬死苗的影响

采用D-饱和最优设计法对播期和播量耦合与小麦越冬死苗的关系进行了研究,结果表明:在10月25日播种、播量为8万基本苗/667 m2条件下,小麦的越冬死苗率最低;晚播和减少播量有利于降低越冬死苗率;播期对小麦越冬死苗率的影响大于播量。     

海湾泥碱渣和粉煤灰作为园林种植基质的氮素生理类群及生化作用研究

海湾泥、碱渣和粉煤灰是天津滨海地区大量存在的固体废弃物,通过前期室内配比筛选、盆栽试验、田间试验表明其混合基质可以用于园林绿化,并在防护林建设中获得成功.以滨海盐土、客土绿地为对照,对通过脱盐、培肥、种植毛白杨、刺槐、洋白蜡、金银木、紫花苜蓿等园林植物后基质的氨化细菌、好气性自身固氮菌、硝化细菌、反硝化细菌、纤维素分解菌等土壤氮素生理类群的数量进行了研究,同时对呼吸作用、氨化作用强度以及二者与土壤微生物数量、理化性质进行了相关性分析.结果表明,基质的氮素生理类群数量高于或与客土绿地相当,明显高于滨海盐土.呼吸作用高于客土绿地和滨海盐土,氨化作用强度与客土绿地相当,说明混合基质的氮素转化、有机物质的矿化能力以及基质整体的代谢水平好于客土绿地.土壤呼吸作用强度与土壤盐分呈极显著负相关,与细菌数量呈显著正相关.氨化作用强度与全氮、全磷、有机质含量、细菌和真菌数量呈极显著正相关,与放线菌呈显著正相关,与速效钾、盐分呈显著负相关.     

有机培肥土壤对优势流中养分淋失的影响

为研究有机培肥对土壤优势流中养分淋失的影响,采用原状土柱模拟淋溶的方法,对土壤优势流中养分淋失的情况进行研究.结果表明,土壤经有机培肥后,明显降低了优势流中各种养分的淋失速度,从而使养分的淋失总量减少,为未施秸秆处理的1/3左右.有机培肥土壤对于淋溶液中养分浓度的影响表现为,在淋溶液中养分浓度总体降低的同时,各种养分的浓度降低的幅度又有所不同,其中对水溶性K和铵离子的效果最明显,而对硝态氮（NO-3-N）保持作用则相对差些.上述结果说明,有机培肥对减少土壤优势流造成的土壤养分淋失以及降低地下水污染都有一定的作用.     

岱海表层沉积物中氮素的水平分布及环境意义

针对岱海湖泊富营养化日趋严重及其水域面积逐渐萎缩的现实,本文系统地研究了TN、NH4^＋-N、NO3^--N和Org—N在岱海表层沉积物中的空间分布规律及其环境意义。结果表明,岱海表层沉积物中氮素的水平分布格局均体现出西北部、北部和东北部为高值区的特征,揭示出岱海的氮污染源主要分布于北岸和西北岸;岱海表层沉积物中氮的主要存在形式为有机氮,铵氮则为无机氮的主要存在形态;表层沉积物中全氮对底栖生物已经具有最低级别的生态毒性效应,对底栖生物群落及生态环境构成了威胁。