基于激光雷达扫描的料仓原料储料量测量方法

为提高仓内原料储料量测量自动化和智能化水平,设计了一种基于二维激光雷达扫描的储料量测量装置与系统。本系统采用RPLIDAR S1型二维激光雷达扫描获取不同储料量物料的原始点云数据,通过坐标变换、重叠点提取、滤波、分割等方法对原始点云进行预处理,采用贪婪投影三角化算法将预处理后的点云进行三维重建,获得仓内原料的三维模型,结合物料三维模型和物料的容重获得仓内原料的储料量,从而实现储料量的自动测量。以玉米为试验对象,测量小型储料塔内玉米储料量并对玉米不同储料量进行扫描测量,验证模型的准确性,结果显示：测量结果的平均绝对误差为8.05 kg,平均相对误差为1.52%。研究结果表明,基于二维激光雷达扫描的储料量测量方法是可行的,具有较好的稳定性和测量精度,能够满足实际生产需求。     

利用连锁不平衡进行QTL精细定位的策略

近几年,随着人类基因组计划的完成和大量DNA分子标记的出现,使得在分子水平上利用连锁不平衡进行QTL精细定位进入了一个新的阶段。应用于人类疾病基因定位领域中的方法逐渐地移植到畜禽的QTL定位中,同时结合畜禽的特点对精细定位进行了研究。本文主要介绍了基于连锁不平衡原理同源相向检测、传递不平衡检测和系谱传递不平衡检验等方法,并对这些方法进行了对比和展望。     

‘阳光玫瑰’葡萄果锈发生规律调查与防治建议

以‘阳光玫瑰’葡萄为调查对象,以果袋颜色、植物生长调节剂、负载量、氮钙施肥配比、挂树时间、树龄、果粒大小、糖度等指标为变量,连续多年在河南省调查了‘阳光玫瑰’葡萄果锈的发生规律,并提出了防治措施,即:套绿色或蓝色果袋;使用25 mg/L GA3+5 mg/L CPPU或25 mg/L GA3+2.5 mg/L TDZ进行无核保果处理;667 m^2产量控制在1500~2000 kg;高钙低氮配比施肥;果实糖度达到18%以后及时采收;培养壮树;多留副梢,尤其是果穗附近副梢叶片;减少或不要触碰果实。     

Roles of protein phosphatase 4 in down-regulation of eNOS Ser633 phosphorylation induced by palmitic acid in human umbilical vein endotheli?al cells

AIMTo investigate the roles of protein phosphatase 4 (PP4) in down-regulation of endothelial nitric oxide synthase (eNOS) Ser633 phosphorylation induced by palmitic acid (PA). METHODSHuman umbilical vein endothelial cells (HUVECs) were treated with PA at 25 μmol/L, 50 μmol/L, 100 μmol/L and 200μmol/L for 36 h, or treated with PA at 100 μmol/L for 12 h, 24 h, 36 h and 48 h. Protein phosphatase 2A (PP2A) family inhibitor fostriecin (FST, 20 nmol/L) or okadaic acid (OA, 5 nmol/L) was selected to pretreat the HUVECs for 30 min. Protein phosphatase 4 catalytic subunit (PP4c) siRNA or protein phosphatase 2A catalytic subunit (PP2Ac) siRNA was transfected into the HUVECs. The protein expression levels of of eNOS, PP4c and PP2Ac, as well as the level of eNOS Ser633 phosphorylation, were detected by Western blot. The intracellular nitric oxide (NO) content was measured by DAF-FM DA. RESULTS(1) Compared with control group, the levels of eNOS Ser633 phosphorylation were decreased in PA groups in which the HUVECs were treated with 25 μmol/L, 50 μmol/L, 100 μmol/L and 200 μmol/L PA for 36 h (P<0.05) and 100 μmol/L PA for 24 h, 36 h and 48 h (P<0.05). No significant difference in the level of total eNOS protein expression among all the groups was observed. (2) Compared with control group, both FST and OA pretreatment reversed the reduction of eNOS Ser633 phosphorylation (P<0.05) and the decrease in intracellular NO content (P<0.05) induced by PA. No significant difference in the level of total eNOS protein expression among all the groups was observed. (3) Compared with si-Control group, the PP4c protein expression was significantly reduced (P<0.05), while the level of eNOS Ser633 phosphorylation was significantly increased in si-PP4c group (P<0.05). Although the levels of PP2Ac protein expression declined significantly (P<0.05), the level of eNOS Ser633 phosphorylation remained unchanged in si-PP2Ac group. No significant differencein the level of total eNOS protein expression among all the groups was found. CONCLUSION PA significantly reduces the level of eNOS Ser633 phosphorylation and the content of NO in the HUVECs, which may be due to PA inducing the activation of the PP2A family member PP4 rather than PP2A.     

酿酒山葡萄新品种‘紫晶甘露’

‘紫晶甘露’是以优质酿酒山葡萄酒品种‘左山二’为母本,‘哈桑’为父本杂交选育而成的能够单品种酿造干红酒的山葡萄新品种。果穗圆锥形,有岐肩或副穗。果实圆球形,果皮黑色,果肉绿色,无肉囊,种子2 ~ 4粒。生长势强,抗寒、抗病性极强,4年丰产,4 ~ 6年平均产量1 876 kg • km-2。果实可溶性固形物含量达到21.0%,总酸11.6 g • L-1,出汁率65.5%。     

浅谈广西防控禽流感面临的问题与对策

禽流感(Avian Influenza,AI)是由A型流感病毒引起的禽类传染病.根据致病性的不同,禽流感可分为高致病性、低致病性和无致病性三种.高致病性禽流感是世界动物卫生组织(OIE)规定的A类传染病,同时被列入国际武器公约动物类传染病名单,我国将其列为一类动物疫病.     

不同栽培基质对无芒隐子草种子萌发和幼苗生长的影响

【目的】无芒隐子草（Cleistogenes songorica）是一种抗旱能力较强的草种,但因其种子有休眠现象,成苗周期长,导致无芒隐子草在田间种植的出苗率和存活率不高,本研究旨在解决这一问题。【方法】采用基质栽培技术,选择来源广泛易获得的7种基质（泥炭、锯末、细砂、菇渣、蛭石、珍珠岩、陶粒）对无芒隐子草进行培育,并测定了各基质的理化性质以及无芒隐子草的萌发指标和生长指标。【结果】细砂、泥炭、锯末以及蛭石培育的无芒隐子草在发芽势、发芽率、发芽指数、活力指数以及苗长等指标上均优于其他3种基质,其中锯末、细砂、泥炭和蛭石培育的无芒隐子草发芽率分别比菇渣提高了6.4倍、6.3倍、5.9倍和5.8倍。【结论】通过隶属函数法对各基质进行综合评价,排序为锯末>蛭石>细砂>珍珠岩>泥炭>菇渣>陶粒,推荐使用细砂、泥炭、锯末和蛭石作为无土栽培基质培育无芒隐子草。     

碳交易视域下养殖户畜禽粪污资源化利用受偿意愿与影响因素——基于河北省7市694户养猪户调研数据

为进一步探究养殖户粪污资源化利用受偿意愿、受偿水平及其影响因素,基于碳交易视角,利用2023年3—4月对河北省7市694户养猪户的调研数据,运用条件价值评估法、二元Logit模型和Tobit模型对此进行系统分析。结果表明：1）88.76%的养猪户愿意接受补偿,其受偿水平在25.03~31.50元/t;2）养猪户年龄、养殖场地面积以及粪污资源化利用生态效益等因素会显著影响养猪户受偿意愿;风险偏好、场地面积、养殖规模、养殖信息获取渠道、粪污资源化利用经济效益显著影响其受偿水平;3）规模异质性视角下,粪污资源化利用生态效益显著正向影响不同规模养猪户受偿意愿。自动化设施配置数量和粪污资源化利用社会效益显著影响不同规模养猪户受偿水平,但其影响方向存在显著差异。为使养殖户更好参与资源化利用,应加强对畜禽粪污资源化利用正面效果的宣传,将宏观政策规范与地方实际相结合、因地制宜,有序推进碳交易补偿机制的建立。