创和谐社会,构建学习型高校图书馆

学习型组织为和谐社会的创立带来了全新的管理理念,从学习型组织理论的五项修炼谈起,分析了学习型图书馆能充分发挥馆员的潜力,提高工作机理,提出了创建学习型图书馆的模式。     

关于《中图法》第四版现代舞、交际舞及体育舞蹈类目设置的探讨——兼与张晶老师商榷

针对现代舞、交际舞、体育舞蹈等类图书归类时出现的归类混乱的问题进行了详细分析,指出了《中图法》四版在此类类目设置的不足之处,并提出了解决问题的办法。     

基于OPAC日志的读者需求分析

OPAC日志中详细记录了读者的访问活动,通过对OPAC日志的深入分析,可挖掘出读者的各种需求信息。为了更好地了解读者的需求。实现对图书馆决策的支持,本文以汇文的OPAC系统为例深入研究了OPAC日志分析的方法和过程。并给出了具体的实现方法、分析实例和结果。     

湖南永州地区钙质页岩土壤动物群落多样性研究

通过对永州不同生境设立5个样区进行取样调查,共获得土壤动物2024头,隶属于5门、10纲、31目、111科、共152种。其中垫刃目、弹尾目、蜱螨目属于优势类群;矛线目、膜翅目等14个目属于常见类群,构成了永州土壤动物的主体。不同生境中土壤动物的种类和个体数量都有一定的差异,苗由和杉树林远远大于其它3个生境。土壤动物的垂直分层也比较明显,种类和个体数量随着土壤层次的加深而减少,具有明显的表聚性。对各生境的密度一类群指数DG、丰富度指数D、多样性指数H、优势度指效c、均匀性指数E和相似性系数进行分析,结果表明,植被类型、土壤有机质含量是影响土壤动物群落结构的主要生态因子。     

四川主要粮食作物生产成本收益分析

粮食生产是四川农村家庭收入的重要来源之一,降低粮食生产成本是增加农民收入的重要途径。本文利用四川省农经总站对岳池、仁寿、渠县、泸县、平武、简阳、新都7个县(市、区)的120户农户连续11年的定点调查数据,比较分析了四川水稻、小麦、玉米和红苕等4种主要粮食作物的生产成本、产值和收益等,提出了提高粮食生产效益的建议。     

茶叶中EGCG改善肥胖大鼠肠黏膜屏障损伤的作用研究

为探究EGCG对高脂诱导肥胖大鼠肠黏膜屏障损伤的保护作用,将雄性SD大鼠随机分为正常组、肥胖组、EGCG低剂量及高剂量干预组,每组8只.通过分析大鼠的体重、血清指标、脏器指数及脂肪组织病理切片评估肥胖大鼠脂质聚集与炎症状况及EGCG的干预作用.通过分析肠道病理切片、绒毛长度/隐窝深度值(V/C值)以及血清中脂多糖(LP...     

不同光质LED光照对草菇菌丝生长速度、菌丝分支与生物量的影响

通过探索LED光源在草菇栽培上的精准化应用,为工厂化草菇栽培提供理论依据.以草菇V23为材料,设置LED光质6个处理:对照白光(CK)、红/蓝=1:1、红/蓝=1:2、红/蓝=2:1、单红光、单蓝光,研究不同光质对草菇菌丝生长的影响.结果发现,红光与蓝光的比为1:1菌丝长势表现为较强,对其他指标影响不明显.不同LED光...     

Effects of bFGF on brain edema,nerve function injury and autophagy related protein in rats with traumatic brain injury

AIM:To study the effects of basic fibroblast growth factor (bFGF) on brain edema, nerve function damage and autophagy related proteins in rats with head injury. METHODS:The rat model of craniocerebral injury (CI) was constructed. The rats were divided into control group, CI group, and low-, middle-and high-dose bFGF groups (n=10). The CI model was established in CI group, while the rats in control group were not given epidural impact. The rats in low-dose, middle-dose and high-dose bFGF groups were given bFGF at 2, 4 and 6 μg, respectively, by intraperitoneal injection after 30 min. The neurological function in the rats was evaluated by improved neurological function scoring. The rat brain tissues were taken, and the water content was detected. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β in the brain tissue were measured by ELISA. The malondialdehyde (MDA) content was analyzed by thiobarbituric acid method. The activity of superoxide dismutase (SOD) was examined by WST-8 assay. The glutathine peroxidase (GSH-Px) activity was detected by colorimetric method. The protein levels of autophagy related proteins LC3-Ⅱ and beclin-1 in the brain tissues were determined by Western blot. RESULTS:The neurological function score was increased significantly of the rats in CI group. The rat model of craniocerebral injury was successfully constructed. Neurological function scores in the rats in low-dose, middle-dose and high-dose bFGF groups were reduced, the water content of the brain tissue was also reduced (P<0.05). The levels of TNF-α, IL-6 and IL-1 β were decreased in the brain tissues (P<0.05), the content of MDA was declined (P<0.05), the activities of SOD and GSH-Px were increased (P<0.05), the protein levels of LC3-Ⅱ and beclin-1 were decreased, compared with the untreated rats in CI group (P<0.05). CONCLUSION:bFGF improves the nerve function of the rats with craniocerebral injury, reduces the water content of the brain tissue, reduces the expression of autophagic protein LC3-Ⅱ and beclin-1.The mechanism is related to the inhibition of inflammatory reaction and oxidative damage.     

pBBR1MCS2-Tac-EGFP广宿主载体适宜标记Ralstonia so-lanacearum

利用标记基因追踪病原菌在植物体内的入侵和定殖,是研究病原菌-寄主互作的重要手段.本研究利用电转化法将广宿主载体pBBR1MCS2-Tac-EGFP导入青枯雷尔氏菌(Ralstonia solanacearum)GMI1000菌株中,获得了青枯雷尔氏菌带绿色荧光标记的转化子.转接试验结果表明,转化子的抗生素抗性和绿色荧光...     

绞股蓝RIP基因双子叶植物表达载体的构建及其对烟草叶盘的转化

将绞股蓝(Gynostemma pentaphyllum)核糖体失活蛋白(Ribosome-Inactivating Protein,RIP)cDNA序列(GenBank登录号AY279219)插入到pKYLX71:35 S^2植物表达载体的HindⅢ/SacI位点处,构建了适于在双子叶植物中表达的载体,并经三亲交配法转入土壤农杆菌LBA4404,用于转化烟草品种K-326,分子鉴定表明绞股蓝RIP基因已经整合到再生烟苗的基因组中并发生了转录。