QuEChERS-三重四极杆串联质谱测定柑橘中农药基质效应的方法研究

[目的]针对柑橘中常用的农药,采用QuEChERS方法和超高效液相色谱-三重四极杆串联质谱,系统研究了其测定过程中的基质效应.[方法]柑橘样品利用乙酸-乙腈溶液(V︰V=1︰99)提取、GCB净化,采用甲醇-0.1％甲酸水溶液(含1mmol·L-1乙酸铵)作为流动相,正离子多反应监测(MRM)模式,基质外标法定量,可以...     

穗生长发育阶段水分逆境对超级稻叶片丙二醛含量的影响

通过水分仪结合称重法人为控制土壤水分的盆栽试验,研究了穗生长发育阶段水分逆境胁迫条件对不同类型超级稻叶片丙二醛(MDA)含量的影响。结果表明:在幼穗分化开始至分化后30 d,不同时段的水分逆境均导致叶片丙二醛含量明显增加,加速了叶片膜脂过氧化,并且所受影响的程度随生育时间的增加、控水天数的延长以及叶位的降低而加重。水分逆境导致叶片尤其是下部叶片膜脂过氧化作用增强,丙二醛含量显著升高,倒三叶丙二醛含量最高,增加的程度也最大,其次是倒二叶,剑叶相对较低。不同品种受水分逆境影响的程度不同,普通杂交稻汕优63受影响较大,其次是超级稻Ⅱ优602,超级稻两优培九和国稻6号受影响相对较小,对水分逆境的敏感性不同品种表现有差异。     

贮藏温度对蒲公英采后生理和品质的影响

研究贮藏温度对蒲公英采后生理指标和品质的影响,探索适宜的贮藏温度,为蒲公英贮运技术的发展与延长供应期提供指导。设置25,10,2,-18℃4种贮藏温度,测定了蒲公英在贮藏过程中外观、营养成分含量和采后生理指标的变化。结果表明:采后蒲公英随着贮藏天数的增加,叶片逐渐黄化,粗纤维的含量升高,维生素C和绿原酸含量下降,硝酸盐含量先升高后降低;2℃下贮藏能够使采后蒲公英具有较高的叶绿素、维生素C、绿原酸,较低的硝酸盐和粗纤维含量,较低的细胞膜渗透率、MDA含量和较高的SOD活性,显著地延缓其衰老进程,延长贮藏寿命。     

The peroxisomal matrix shuttling receptor Pex5 plays a role in FB1 production and virulence in Fusarium verticillioides

The peroxisomal matrix oxidase,catalase and peroxidase are imported peroxisomes through the shuttling receptors,which regulates the cellular oxidative homeostasis and function.Here,we report that PTS1 shuttling receptor FvPex5 is involved in the localization of PTS1,utilization of carbon sources and lipids,elimination ROS,cell wall stress,conidiation,fumonisin B₁(FB₁) production,and virulence in maize pathogen Fusarium verticillioides.Significantly,differential expression o...     

香蕉MaSULTR3家族成员的全基因组鉴定与不同温度下表达模式分析

利用生物信息学分析法对香蕉MaSULTR3基因家族成员进行全基因组鉴定、蛋白特性分析、分子进化树分析、启动子顺式作用元件分析以及低温胁迫下叶片转录组的FPKM值分析;其后,利用基因组数据结合RT-PCR方法克隆了'天宝蕉'(Musa spp.,AAA Group)组培苗MaSULTR3.1-2基因的ORF序列;最后,利...     

The effect of glucocorticoid receptor on level of inflammatory media in rats after critical scald

AIM:To further elaborate the effect of glucocorticoid receptor(GR) decrease on level of inflammatory media in rats after critical scald.METHOD:The changes of phospholipase A₂(PLA₂) activity and concentrations of tumor necrosis factoralpha (TNFα) and malondialdehyde (MDA), the product of lipid peroxidation metabolism in plasma and tissue homogenate have been studied in scalded rats with or without GR blockade by RU38486.RESULTS:The PLA₂ activity and the concentrations of TNFα and MDA in plasma and homogenate of pulmonary and renal tissue in scalded rats were significantly higher than those in the controls(P＜0.01) and further increased in scalded rats accompanied with GR blockade (P＜0.01 or P＜0.05).CONCLUSION: Blockade of GR might aggravate the increase of inflammatory mediator caused by scalding. It suggested that GR might potentiate anti-inflammation in scald.     

Cardiac L-type calcium channel and its alterations in cardiac hypertrophy and heart failure

There are two type of cardiac calcium channel current, both are inward current: (1) L-type Ca²⁺channel current (I_ca-L), plays a major role in determining myocyte action potential plateau characteristics as well as initiating the myocyte excitation-contraction coupling; (2) T-type Ca²⁺ channel current (I_ca-T), probably plays an important role in pacemaker activity. The alterations of L-type calcium channel abundance and function in cardiac hypertrophy and heart failure are determined by the the species difference, especially by the stage of the disease process and the degree of the disease. Both abundance and function of L-type calcium channel decrease in severe hypertrophy and heart failure.     

Adhesion of bone marrow stromal cell in children with acute lymphoblastic leukemia

AIM and METHOD: The adhesion behavior of bone marrow stromal cells(BMSC) in children with acute lymphoblastic leukemia(ALL) to bone marrow mononuclear cells(BMMC) and Raji cells were investigated by MTT method. The expression of adhesion molecules ICAM-1 and VCAM-1 were detected by flow cytometry. RESULTS: The adhesion ability of BMSC in acute period of ALL to BMMC was lower than that of control group. The adhesion ability of BMSC in long term remission period of ALL to Raji cells higher than that of control group. The expression of ICAM-1 on the surface of BMSC in acute period of ALL is much lower than that of control group. CONCLUSIONS: The adhesion of BMSC to BMMC or tumor cells in children with ALL was abnormal. The abnormal adhesion behavior might be partially due to the changed expression of adhesion molecules on BMSC.     

Protective effect of C1q/tumor necrosis factor-related protein 3 on vascular endothelium in rats with hyperuricemia

AIM To study whether C1q/tumor necrosis factor （TNF）-related protein 3 （CTRP3）protect vascular endothelium in rats with hyperuricemia and its potential mechanisms. METHODS An animal model of hyperuricemia was established by using male SD rats drinking 10% fructose water （n=10）. The rats drinking normal water served as normal controls （n=10）. After 12 weeks, the rats were given a single injection with Ad-CTRP3 or Ad-GFP. The experiment was ended at 14th day after transfection.The serum levels of uric acid and nitric oxide （NO） were evaluated. The serum contents of TNF-α and interleukin-6 （IL-6） were measured by ELISA. HE staining and TUNEL assay were used to assess the morphological changes of intima and apoptosis of endothelial cells in thoracic aorta, respectively. The mRNA levels of endothelial nitric oxide synthase （eNOS）, TNF-α and IL-6 were detected by RT-qPCR. The protein levels of CTRP3 and Toll-like receptor 4 （TLR4） were determined by Western blot. RESULTS Compared with normal control group, the rats with hyperuricemia showed lower CTRP3 and higher TLR4 protein levels in the thoracic aorta （P<0.05）. Hyperuricemic rats had higher serum contents of uric acid, TNF-α and IL-6 （P<0.05）. Also, the intima structure disturbance of thoracic aorta, increased apoptotic rate, higher mRNA levels of TNF-α and IL-6 as well as lower mRNA levels of eNOS were observed （P<0.05）. By contrast, CTRP3 over-expression decreased TLR4 protein levels, reduced inflammatory cytokines, and obviously improved the morphology and function of thoracic aorta in the rats with hyperuricemia. CONCLUSION CTRP3 protect vascular endothelium in rats with hyperuricemia maybe via down-regulation of TLR4- mediated inflammatory signaling pathway.     

盐度变化对军曹鱼稚鱼相关免疫因子及其生长的影响

研究了盐度(5、10、20、30及对照37)对军曹鱼(Rachycentron canadum)稚鱼生长及其血清溶菌酶、碱性磷酸酶(ALP)、旁路途径补体(ACP)溶血活性(ACH50)和总免疫球蛋白(T-Ig)含量等免疫因子的影响。实验周期为14 d。结果表明,在逐渐达到设定盐度后养殖14 d,盐度30组稚鱼特定生长率(SGR)最高(5.77%/d),而盐度5和10组的SGR(分别为4.24%/d和4.38%/d)显著低于其他组(P<0.05)。在低盐度环境中血清溶菌酶活性在第7天各组都有不同程度升高,其中以盐度20组升高最为显著,其次为盐度10和30组,但第14天各组溶菌酶活性又都回落至对照组水平;而稚鱼在低盐度环境中血清ALP活性受到明显抑制,且活性与盐度呈一定正相关;各组间ACP活性在第7天各盐度组间无明显差异,但实验结束时盐度5和10两组出现显著升高;而T-Ig含量在盐度10组中始终明显高于其他组。研究显示,军曹鱼稚鱼在盐度20~37范围内都可正常生长,尽管稚鱼有较强的低盐度耐受能力,但过低盐度明显影响其生长率并导致体色变黑、蛀鳍和应激增强等异常表现。此外,盐度变化还影响稚鱼多种免疫相关因子。[中国水产科学,2007,14(1):120-125]