野生朱红硫磺菌驯化栽培研究

营养试验、菌丝体培养和出菇温度试验、培养基酸碱度试验、出菇条件试验结果表明：朱红硫磺菌菌丝体适宜生长的温度范围为15-32℃，最适温度范围为22～28℃；子实体发生的最适温度为16～25℃，气温低于16℃，子实体色泽为淡橙色，16℃以上，子实体呈鲜橙红色；培养料适宜营养C／N为20-26／1；朱红硫磺菌菌丝体适宜酸碱度范围为pH3．0～8．0，最适酸碱度为pH4．0—6．5；朱红硫磺菌的产量与光照条件无关，但光照强度影响子实体的发生时间和色泽；朱红硫磺菌栽培原料广泛，凡栽培木腐菌的培养料均可用于栽培朱红硫磺菌。     

彩色甜椒花药培养若干影响因子的研究

利用硝酸银、ATP钠盐、NAA、KT进行了四因素三水平正交试验,材料为彩色甜椒品种7160的花药。结果表明,硝酸银是影响彩色甜椒花药培养胚状体诱导率的最主要因素,在其浓度为1.0mg/L时胚状体诱导率最高;ATP钠盐为次主要因素,在其浓度为100.0mg/L时效果最好。KT比NAA对胚状体诱导率的影响大;KT浓度1.0或1.5mg/L时较好。     

高寒地区不同建植期人工草地群落垂直结构和生产力变化的研究

对高寒地区不同建植期人工草地群落垂直结构和生产力的变化研究结果表明：（1）随着人工草地建植时间的延长,人工草地群落垂穗披碱草高度异质性逐渐减小.（2） 不同建植期（1999、1998、1997年建植）人工草地垂穗披碱草和群落平均地上生物量均主要分布在0 ～20cm冠层中,约占平均地上生物量的61.03%、90.64%、84.55%和67.47%、92.54%、86.08%.（3） 不同建植期 （1999、1998、1997年建植）人工草地群落平均地下生物量均主要分布在0～10cm土层中,约占地下总生物量的85.97%、81.73%和78.47%.（4） 建植的人工草地如果其组分单一,即物种的丰富度很低时,均匀度增加,物种对环境资源的竞争力和利用率提高,导致土壤资源库中某些营养成分缺乏,草地群落初级生产力因此而降低。     

塔里木河流域生态环境恶化的水文效应

塔里木河是我国最大的内陆河。 4条源流出山口多年平均天然径流量 2 2 4 .9× 10 8m3 (195 7～ 2 0 0 1年 )。在全球变暖的大背景下 ,塔里木河流域山区气候出现变暖增湿的趋势 ,2 0世纪 90年代径流量达 2 4 1.9× 10 8m3 ,增幅 7.6 %。由于源流区人类开发利用水资源的影响 ,使得补给塔里木河干流的水量不断减少 ,水文条件改变 ,造成干流上、中游段耗水严重 ,导致下游生态与环境急剧退化。自 2 0 0 0年以来 ,实施的从开都河—博斯腾湖向下游绿色走廊应急输水 ,已使下游生态环境开始恢复生机。     

Protective effect of onychin on the human umbilical vein endothelial cells injured by menadione

AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO₂^-／NO₃^- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO₂^-／NO₃^- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway.     

Effect of CGRP on level of lung endogenous NO in rabbits with acute lung injury

AIM:To examine whether calcitonin gene-related peptide (CGRP) enhances nitric oxide (NO) level in pulmonary circulation blood and observe the influence of CGRP on mean pulmonary artery pressure (mPAP) in rabbits with acute lung injury (ALI) caused by oleic acid.METHODS: The level of NO was assessed by measuring the presence of nitrite in cervical artery blood by the Griess reaction, mPAP was measured with right ventricular catheter.RESULTS:The level of nitrite in cervical artery blood was significantly increased and the mPAP was markedly reduced after administration of CGRP intravenousely.CONCLUSION:CGRP enhanced the NO level of pulmonary circulation blood and reduces the mPAP significantly in rabbits with ALI.     

The transdifferentiation of Sca-1+ cells from murine fetal liver

AIM:To explore transdifferentiation potential of Sca-1⁺ cells from murine fetal liver. METHODS:2×10³ of Sca-1⁺ cells from male murine fetal liver were transfused into female mouse irradiated lethally with γ ray from ⁶⁰ Co source (10 Gy) via tail vein. Two months later, FISH and immunohistochemistry were used to detect the situation for transdifferentiating of the donor cells (male cells) in tissues of female recipient mouse. RESULTS:The renal tubular epitheliocyte-like and neurocyte-like cells with Y chromosome were found on the sections of renal and brain tissues from female recipient mice. These cells have phenotype characteristics of RCA⁺/CD₄₅^-F_4/80^- and NueN⁺/CD₄₅^-F_4/80^-, respectively. CONCLUSION:The evidence is provided for Sca-1⁺ cells from murine fetal liver to transdifferentiate into both renal and brain tissue cells.     

蘑菇培养料集中二次发酵初步研究

本文设计和建造小型二次发酵隧道 ,并就蘑菇培养料集中二次发酵不同填料高度工艺进行详细的研究 ,试验结果发现采用 1 8米的填料高度的发酵方法效果最好 ,产量较一般简易二次发酵方法提高 2 9%。     

我国小麦禾谷镰刀菌(Fusarium graminearum)营养体亲合性与致病性研究

 赤霉病是在世界范围内多种作物上反复发生的病害,小麦受害尤其严重。     

散养湘黄鸡常见病的临床表现特点及其防制

湘黄鸡进行专业户(场)散养时，由于生产方式不同于舍饲群养，其生产环境、食物结构和易感病种类都必然发生变化。对此，笔者结合临床所遇病例进行了研究总结，对散养湘黄鸡的几种常发病的病因、病理、临床表现及防治特点等方面进行了介绍。