贵州发现黑颈长尾雉(Syrmaticus humiae)

Introduction Hume’s Pheasant (Syrmaticus humiae), including two subspecies, is distributed in northeastern India, Myanmar, northern Thailand and southwestern China (Collar et al., 2001). In China its distribution has been assumed to be restricted to Yunnan and northwestern Guangxi (Mackinnon et al., 2000; Zheng, 2011).     

不同的干燥工艺对荷花蜂花粉功能成分的影响

探讨了5种干燥工艺对荷花蜂花粉功能成分的影响。微波干燥的干燥效率最高,60%火力处理540s可使水分从31.41%降到6.42%;干燥方式对荷花蜂花粉蛋白质影响较小;微波干燥后总黄酮提取率最高,为13.20mg/g;真空冷冻干燥后维生素C和过氧化氢酶含量最高,分别为48.89mg/100g和29.86mg/g·min。总的来说,真空冷冻干燥对荷花蜂花粉的功能成分影响最小;常压恒温干燥对功能成分影响较小、工艺简单、对设备要求低,宜于大规模应用于实际生产中。     

家蚕母种继代蛾区选择方法对种性维持的影响

为了有效保持生产实用家蚕品种的优良性状,在家蚕母种继代繁育中采用5种选择方法选择继代蛾区,探讨种性维持效果。分析8年12代及F1代的成绩表明:所有性状以保持种性为主,采用选留平均数上下1个标准差范围内蛾区的稳定化选择和以茧丝质指标在平均数-0.5~1.0标准差范围内选择,并结合健康性指标选留高值的综合选择,有利于家蚕母种的种性维持。     

表达新城疫病毒F基因重组鸡痘病毒的筛选

将转移载体P11SF和PN11SF分别与282E4株鸡痘病毒（282E4 strain fowlpox virus，282E4FPV）和大空斑株鸡疽病毒（large plaque strain fowlpox virus，LP FPV）共转染鸡胚成纤维细胞，通过蓝斑筛选，得到4株重组鸡痘病毒rFPV282E4-SFA、rFPVLP，-SFA、rFPV282E4-SFB和rFPVLP-SFB，通过PCR和间接免疫荧光鉴定均为阳性，另外将各重组病毒分别传20代，测其遗传稳定性，结果显示均具有良好的稳定性。对SPF鸡进行免疫效力试验，攻毒后均100％保护，而对于商品鸡，保护率分别为64％、60％、52％和88％。     

Testing and Identification of Carp Edema Virus Disease in Cultured Koi

An acute infectious diseases occurred in a koi farm in Fangshan district, Beijing, and it resulted in mortalities of more than 50%.The main symptoms of sick koi were gills necrosis, kidney erosion and edema,which were similar to the clinical signs of koi herpes virus disease (KHVD).But PCR tests showed negative results for KHV. For further diagnosis, bacterial cultures, transmission electron microscopy studies, virus isolation and PCR tests were used. Electron microscopic observation revealed pox virus particles having a size of about 200 nm×400 nm in the kidney. 548 and 180 bp fragments were amplified from organs of sick koi by PCR method using specific primers of carp edema virus (CEV). The fragments were sequenced and analysed. The results showed that they were shared 100% nucleotide identity with CEV-H504. All the results indicated that this disease was carp edema virus disease, caused by a kind of pox virus, CEV. This was the first report on the CEV of cultured koi in China.     

黄墟镇禽用浅井水质调查

普查镇江市黄墟镇104口浅井水质,并长期监测了其中7口的水质。结果表明：pH值超标率为11．5％,总硬度仅有1口超标,且氯化物含量较低,属中性软水和低度碱性硬水区域;氨氮超标率70．4％,为主要面源污染物;监测的七口井氨氮浓度在七、八月份和十一、十二月份比较高,COD随着时间的推移呈现增加趋势,氯化物含量很低,且与总硬度呈线性关系,凸现种植业污染特征。     

家蚕表皮蛋白基因的生物信息学分析

家蚕的表皮蛋白(cuticular protein)是家蚕重要的结构蛋白,与几丁质一同作为家蚕表皮的重要组成部分。运用生物信息学的方法对家蚕表皮蛋白进行了广泛分析。通过保守的基序检索家蚕基因组,得到163条候选的表皮蛋白序列,包括RR、Tweedle、CPF&CPFL等家族,并且发现具有确定的保守基序的表皮蛋白基因在染色体上都是以串联集群形式分布。氨基酸组成分析表明,这些表皮蛋白富含甘氨酸、丙氨酸、亮氨酸、组氨酸等。运用Sig-nalP server预测这部分蛋白质有85%都具有信号肽。进化分析显示,负选择是家蚕表皮蛋白基因进化的主要驱动力。采用TFSEARCH等在线服务软件分析发现,在70%含有RR基序的家蚕表皮蛋白基因的核心启动子区具有通用的TATAAA序列。     

A case of perosomus elumbis in a Holstein calf

Perosomus elumbis is an occasionally found congenital anomaly of unknown etiology and is characterized by partial or complete agenesis of lumbar, sacral and coccygeal vertebrae and ankylosis of the hindlimbs. A 2-day-old female Holstein calf presented nearly normal forelimbs but flexure and ankylosis of the hindlimbs. The vertebrae and pelvic malformations and agenesis were radiographed and then necropsied. Mild ankylosis of the hindlimbs, absence of cauda equina, left scoliosis in state of fusion of T11 and T12 and complete fusion of L4 and L5, narrowed pelvic canal and misshapen ilium were confirmed. However, abnormal development or agenesis was not observed in the urogenital and intestinal system in this calf.     

应用单引物PCR指纹技术分析Vc-獭兔的分子遗传结构

采用1条小卫星引物M13(5’GAGGGTGGCGGTTCT3’)和2条微卫星引物(GTG)5、(GACA)4，对Vc—Ⅰ系、Vc—Ⅱ系獭兔、日本大耳白兔(JWR)和青紫蓝(QZL)4个品系各8只，总计32个个体的遗传结构进行了DNA指纹分析。结果，3条引物的扩增产物都呈现良好的多态性和稳定性；对每个品系混合DNA池进行分析，计算出了4个品系间及32个个体间的共有带率及遗传距离指数，通过聚类分析，得出各品系间和个体间欧式遗传距离，并绘制出4个品系间及各品系32个个体问的亲缘关系树状聚类图，从而初步建立了4个品系兔的SPAR指纹图谱。