Proliferation-inhibitory and apoptosis-inducing effects of cinnamic acid combined with cisplatin on human hepatocellular carcinoma cell line MHCC97

AIM：To investigate the effects of cinnamic acid (CA) combined with cisplatin on the proliferation and apoptosis of human hepatocellular carcinoma cell line MHCC97. METHODS：Human hepatocellular carcinoma cell line MHCC97 was culured and divided into CA group, cisplatin group, CA+cisplatin group and control group. MTT assay, inverted microscopy, annexin V-FITC/PI staining and flow cytometry were applied to identify the viability, morphology and apoptosis of the cells. The apoptosis-related signaling protein caspase-3 was detected by Western blotting. RESULTS：CA and cisplatin either alone or in combination significantly inhibited the proliferation and induced obvious apoptosis of MHCC97 cells, while CA alone or combined with cisplatin had no significant inhibitory effect on normal human liver L-02 cells. The rates of mid-and late apoptosis or necrosis were higher in cisplatin group than that in CA group or combination group, but the early apoptotic rate was just the opposite. Pro-apoptotic activity in combination group was much stronger than that in CA group or cisplatin group at lower concentration, and combination group promoted apoptosis and decreased the cytotoxic side effects of cisplatin. CA and cisplatin either alone or in combination also up-regulated the cleaved caspase-3 expression in a time-dependent manner, and the effects in CA group and combination group were higher than that in cisplatin group. CONCLUSION：CA and cisplatin either alone or in combination inhibit the growth of MHCC97 cells by inducing apoptosis, and the activation of caspase-3 may play important roles in these processes.     

Comparison Analysis of Immune Related Gene of Orf Virus in Sheep and Camels

The purpose of this study was to investigate the variation of Orf virus (ORFV) immune related genes after infection with different species.The ORFV genomes of sheep and camel were extracted and named ORFV-Y and ORFV-LT,respectively.Based on ORFV genome sequence published in GenBank (accession No.:KF234407.1),three pairs of specific primers were designed and synthesized to amplify the B2L,F1L and VIR gene fragments of ORFV-Y and ORFV-LT,respectively,and the amplified fragments were cloned into pMD19-T vector,transformed into E.coli DH5α competent cells.The recombinant plasmid was identified,and positive clones were selected for sequencing,DNAStar software was used to analyze the homology,amino acid sequence and phylogenetic tree of 13 ORFV genome sequences published on NCBI.The results showed that the nucleotide homology of B2L,F1L and VIR genes were 92.8% to 99.2%,95.7% to 99.5% and 77.6% to 100%,respectively.After comparing the amino acid sequence between the two genomes and the reference sequence,it was found that there were obvious differences in the immune related genes between the two genomes,and F1L gene had some rules to follow.The phylogenetic analysis of B2L,F1L and VIR genes showed that ORFV-Y was closely related to the Chinese Fujian goat strain,while ORFV-LT was far from the reference strains,and was a separate branch.The results showed that ORFV had obvious difference in immune related genes between sheep and camels,it provided a reference basis for further research on the changes of ORFV gene sequences in different species and the development of vaccines for different species in the future.     

刈割对青海早熟禾再生性的影响

通过对青海草地早熟禾不同刈割强度下再生性的研究,结果表明:刈割高度与频度不同,牧草再生能力不同。低茬高频的刈割,即留茬高度5cm、刈割频度7天/次,青海草地早熟禾再生性达到最佳水平,产草量也随之增加。     

陕西富硒与非富硒地区山羊组织硒含量和GPX-7基因表达的比较

为了探讨陕西紫阳县(富硒地区)山羊各组织中微量元素硒的含量和谷胱甘肽过氧化酶7(Glutathione Peroxidase 7,GPX-7)基因的表达情况,以及组织中的硒沉积量对GPX-7基因表达的影响。利用原子荧光光谱法对年龄相仿、体质量相近的紫阳县双安镇(n=7)和广成镇(n=7)和宝鸡市陈仓区白山羊体内硒含量进行了测定;同时运用实时荧光定量PCR(qRT-PCR)相对定量技术测定GPX-7基因mRNA的相对表达量。紫阳双安镇山羊硒含量显著高于紫阳广成镇(P0.05),同时紫阳县均显著高于宝鸡陈仓区(P0.05);但是紫阳双安镇与紫阳广成镇山羊肺脏中硒含量差异不显著(P0.05)。GPX-7基因在各组织器官中广泛表达,但是不同地区的山羊表现出不同的组织差异性。紫阳双安镇山羊心脏、脾脏、肾周脂肪和背最长肌组织中mRNA水平与紫阳广成镇差异不显著(P0.05),但是紫阳地区均显著高于宝鸡陈仓区(P0.05)。在肺脏、肾脏、股二头肌和淋巴组织中,来自不同地区的山羊GPX-7基因mRNA的表达量地差异均两两显著(P0.05),其中紫阳广成镇山羊肺脏的表达量显著高于其他两个地区(P0.05)。由此可见,山羊各组织器官中微量元素硒的沉积量和GPX-7基因mRNA的表达量表现出较为一致的地域趋势,即紫阳双安镇紫阳广成镇宝鸡陈仓区,这表明微量元素硒上调GPX-7基因的表达,但是具有组织差异性。     

京、津、冀区域扬沙天气气候变化特征分析

利用京、津、冀区域内所辖 6 5个气象观测站 1 95 4 - 2 0 0 2年的观测资料 ,分析了扬沙天气的气候变化特征。结果表明 :京、津、冀区域绝大部分地区是扬沙天气的主要影响区。高原、平原和丘陵地区是扬沙天气的多发区和易发区 ,宁晋、邢台一带是扬沙天气的高频区。扬沙的发生时间具有明显的季节变化特征 ;此区域内的扬沙呈现整体减少趋势 ,各分区域的变化趋势具有相似性 ,只是在减少幅度和年代际减少趋势上略有不同     

蓟马采集和玻片标本的制作

\t\t\t\t\t目的\t\t\t\t\t丰富中国呆蓟马属团蓟马种类,为呆蓟马属团的鉴定提供参考信息。\t\t\t\t\t\t\t\t\t\t\t\t\t方法\t\t\t\t\t标本采集采用拍盘法,标本保存在75%的酒精中,带回室内进行玻片标本制作,在生物显微镜下进行鉴定。\t\t\t\t\t\t\t\t\t\t\t\t\t结果\t\t\t\t\t记述了蓟马亚科呆蓟马属团一中国新纪录属塔姆蓟马属(Tameothrips)及1新纪录种芦竹塔姆蓟马(T. arundo),详细描述了该种的形态鉴定特征,编制了中国已知呆蓟马属团属种名录及分属检索表。玻片标本保存于云南农业大学植物保护学院昆虫系。\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t结论\t\t\t\t\t塔姆蓟马属(Tameothrips)为中国新纪录属,中国目前已记录呆蓟马属团9属21种。\t\t\t\t\t\t\t\t\t     

调亏灌溉和氮处理对小桐子生长及水分利用的影响

为了研究阶段水分亏缺(亏水+不亏水W_LW_H、不亏水+亏水W_HW_L、亏水+亏水W_LW_L、不亏水+不亏水W_HW_H)和不同施氮量(N_Z:0,N_L:0.2 g/kg,N_H:0.4 g/kg)对小桐子生长、生理指标和灌溉水利用效率的影响,通过盆栽试验,发现W_LW_H的生长和灌溉水利用效率均显著高于W_HW_L;作物的灌溉水利用效率随施氮量的增大而呈现先增大后减小的趋势,在N_L水平下达到最大值;与高水高氮的处理N_HW_HW_H相比,中水低氮处理N_LW_LW_H节约灌溉水27%,节约氮肥使用量50%,小桐子株高减少31%、总干物质量减少35%,灌溉水利用效率减少13%,但茎粗增加13%,根冠比增加20%.可见小桐子在第一阶段处理(40~90 d)幼树期对水分的需求量较小,适度的亏缺灌溉可提高灌溉水利用效率;小桐子在第二阶段处理(90~140 d)处于旺长期,对水分的需求量较大,增大灌水量可大幅度促进小桐子生长及其干物质量的积累.全生育期实施亏缺灌溉,可提高小桐子自身适应外界环境能力,抗干旱胁迫能力也逐渐增强,但W_LW_L水平下的小桐子生长缓慢.经综合分析,认为处理N_LW_LW_H可作为干旱地区条件下的小桐子灌溉和施氮制度.     

不同类型有机肥对烤烟石油醚提取物及香气物质含量的影响

采用田间试验方法,研究不同类型有机肥对石油醚提取物及香气物质含量的影响。结果表明:1)施用有机肥均能增加石油醚提取物含量,其中T3(无机化肥+芝麻饼肥+生物有机肥)处理的中、上部叶石油醚提取物含量最大,分别为7.76%、8.75%;2)施用有机肥处理对上部叶的增香效果优于中部叶,有机肥处理的上部叶有24种香气物质含量均高于CK,中部叶有18种高于CK;其中T3处理的中、上部叶致香物质总量最大,分别高达1 291.45、1 524.34μg/g,较CK处理分别增加3.32%、34.86%。     

Molecular cloning and functional analysis of duck Toll-like receptor 5

Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In this study, we cloned the single-exon TLR5 gene of the Maya breed of Common Shelduck (Tadorna tadorna). The TLR5 open reading frame is 2580 bp in length and encodes an 859-amino acid protein. The putative amino acid sequence of duck TLR5 consisted of a signal peptide sequence, 11 leucine-rich repeat domains, a leucine-rich repeat C-terminal domain, a transmembrane domain, and an intracellular Toll-interleukin-1 receptor domain. The duck TLR5 gene was highly expressed in the lung, bone marrow, spleen, and liver; moderately expressed in kidney, small intestine, large intestine, and brain. A plasmid expressing duck TLR5 was constructed and transfected into HEK293T cells, and expression was confirmed by indirect immunofluorescence assay. HEK293T cells transfected with duck TLR5- and NF-κB-luciferase-containing plasmids significantly responded to flagellin from Salmonella typhimurium, indicating that it is a functional TLR5 homolog.     

胡麻异质型ACCase亚基基因的克隆与表达分析

为研究胡麻异质型乙酰辅酶A羧化酶BCCP、BC、α-CT和β-CT四个亚基基因的生物学功能,采用基因克隆、生物信息学和RT-PCR技术分别对胡麻accA、accB、accC和accD 4个基因进行分析。结果发现陇亚10号accA基因编码α-CT亚基,CDS序列全长为2364 bp,编码787个氨基酸;accB基因编码BCCP亚基,CDS序列全长为1173 bp,编码275个氨基酸;accC基因编码BC亚基,CDS序列全长为1574 bp,编码527个氨基酸;accD基因编码β-CT亚基,CDS序列全长为1137 bp,编码378个氨基酸,且4个基因编码的蛋白都是脂肪酸系数较高的亲水性蛋白。RT-PCR结果表明,胡麻accA、accB、accC和accD 4个基因在3个胡麻品种(高含油量的张亚2号、中含油量的陇亚10号和低含油量的R2-17)的不同时期不同组织的表达模式不同,accA、accB、accC和accD四个基因在R2-17、陇亚10号和张亚2号3个胡麻品种所有时期的不同组织中均有表达,但在种子中的表达量均明显高于其他组织,尤其在种子发育10~25 d油脂快速积累时期,4个基因的表达量都迅速增加并达到最高峰。研究表明异质型乙酰辅酶A羧化酶基因可能调控胡麻种子发育前期油脂的合成积累。