彩叶植物金叶莸的组织培养及植株再生研究

对彩叶植物金叶莸进行了初代培养、继代培养、生根培养及驯化移栽等试验.结果表明:初代培养的最佳培养基为MS 6-BA 1.5 mg/L NAA 0.5 mg/L;继代培养的最佳培养基为MS BA 1 mg/L NAA 0.5 mg/L;生根培养的最佳培养基为1/2 MS NAA 0.5 mg/L或1/2 MS NAA 0.25 mg/L IBA 0.25 mg/L;驯化移栽的最佳基质为珍珠岩 腐叶土(1∶1).     

应用轻型飞机防治马尾松毛虫的试验Ⅰ．喷施四种药剂防治的效果

马尾松毛虫Dendrolimus punctatus Walker是马尾松林重要害虫。林间应用超轻型飞机大面积喷洒溴氰菊酯超低容量喷雾、白僵菌纯孢子油剂、菌药混合液超低容量喷雾以及喷洒白僵菌菌粉,其防治效果分别为96．5%、85．7%、95．6%、86．5%。上述的防治措施可作为单独的措施或综合防控中的配套措施使用,同时应用飞机防治解决了树高、林密、水源缺乏、劳力工资昂贵等条件给防治工作带来的困难,是一种适应林区林业生产整体水平而又快速、高效、经济的防治手段。     

Effects of Yeast Culture on Growth Performance,Meat Quality and Intestinal Microflora of Broilers

The experiment was conducted to study the effects of yeast culture on growth performance, meat quality and intestinal microflora of broilers. A total of 432 1-day-old AA commercial broiler chicks with initial body weight of (41.73±0.15)g were randomly divided into 4 groups with 6 replicates per group and 18 broilers per replicate,receiving diets supplemented with 0,0.1%,0.2% and 0.3% yeast culture, respectively. The trial lasted for 42 days which was divided into two periods with 1 to 21 d and 22 to 42 d,respectively.The feed intake,body weight,death and culling rate were recorded in each period. At the end of the experiment,1 chickens in each replicate with the weight close to the average value were slaughtered after fasting for 12 h. The slaughter performance and intestinal microflora counts were determined.The results showed that compared with control group,the supplementation of 0.2% and 0.3% yeast culture significantly increased the body weight at 42 d and the average daily gain (ADG) during the whole trial (P< 0.05).The diet supplemented with 0.2% yeast culture significantly improved the ADG,decreased the F/G of broilers during 22 to 42 d (P< 0.05),and it also significantly increased the average daily feed intake (ADFI) and ADG (P< 0.05),decreased the F/G of the whole period (P< 0.05).Moreover,the supplementation of 0.2% yeast culture significantly decreased the drip loss of meat and the count of Escherichia coli (P< 0.05),and significantly increased the count of Bifidobacterium(P< 0.05). In summary, the supplementation of 0.2% yeast culture could significantly improve the growth performance and the muscle quality of broilers.The intestinal microflora in broiler cecum were effectively optimized after inclusion of yeast culture in the study.     

使用Cytoscape对生物网络数据的建模和分析

生物网络是系统生物学研究的重要内容。本文介绍了多种常用复杂生物网络可视化系统,针对Cytoscape系统在可视化的建模过程以及在建模过程中使用到的布局算法进行对比研究,并利用Cytoscape系统中丰富的插件对复杂网络数据进行分析,包括对数据进行网络分析和更改、使用BINGO插件进行基因注释、使用AGILENT LITERATURE SEARCH(ALS)插件搜索基因相互作用文献等。     

Effect of lipoic acid on redox regulation,digestive and absorptive function in intestine of mice fed high fat diet

AIM:To elucidate the molecular mechanisms of lipoic acid (LA) on redox regulation and digestive function in intestine of C57BL/6 mice fed high fat diet (HFD).METHODS:C57BL/6 mice were randomly assigned to one of three groups (n=8).The control group consumed an ordinary diet.The other two experimental groups were fed with a high fat diet, high fat plus 0.1% LA.After 6 weeks, the activities of digestive enzymes were examined.In order to evaluate the antioxidant status of the mice, superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant capacity (TAC) and reactive oxygen species (ROS) in intestinal homogenate were measured.To investigate the molecular mechanisms underlying the effects of LA, the gene expression profiles in intestine were examined using the GeneChip microarray system.RESULTS:A depressed antioxidant defense system, accompanied by digestive and absorptive function impairment, was observed in HFD-fed mice.These changes were partially restored in the LA-treated group.DNA microarray analysis of intestine showed that LA ingestion up-regulated the expression of genes related to free-radical scavenger enzymes, digestive enzymes and transporters.CONCLUSION:Treatment with LA improves redox homeostasis and the function of intestine in mice fed HFD.The mechanism may involve preventing oxidative stress by scavenging ROS directly and increasing those of free-radical scavenger enzymes gene expression indirectly.     

Pharmacokinetics of cimetidine in dogs after oral administration of cimetidine tablets

Long-term oral treatment with cimetidine is recommended to reduce vomiting in dogs with chronic gastritis. Despite this, few studies have specifically examined the plasma disposition and pharmacokinetics of cimetidine in dogs, particularly following repeated oral administration. The pharmacokinetics of cimetidine following oral administration as tablets was investigated in healthy dogs. Cimetidine was absorbed rapidly post-treatment ( t _max = 0.5 h). A mean absolute bioavailability of 75% was calculated following a single oral administration of 5 mg cimetidine/kg body weight. After intravenous administration, a plasma half-life of 1.6 h was calculated. Repeated oral administration at the recommended dose rate and regime (5 mg/kg body weight three times daily) for 30 consecutive days did not lead to any accumulation of cimetidine in plasma. Food intake concomitant with oral administration of cimetidine delayed ( t _max = 2.25 h) and decreased the rate and extent of absorption ( AUC ) by about 40%. Cimetidine was well absorbed in fasted dogs. Administration of food decreased the bioavailability of cimetidine by 40%. Cimetidine does not accumulate over time in plasma when administered long term to dogs.     

Change of reactive oxygen species production in co-culture model of rat astrocytes and motor neurons VSC 4.1

AIM: To explore the mutual effect of co-culture of wild-type astrocytes (ASC) and motor neurons VSC4.1 (VSC) on the respective ability to produce reactive oxygen species (ROS). METHODS: The inhibition rates of cell growth in ASC and VSC in co-culture or independent culture was detected after exposed to excitatory stimulus by MTT method. Real-time observation of ROS production by ASC and VSC labeled with Hoechst 33342 was detected by confocal microscopy under the conditions of co-culture or independent culture. RESULTS: Higher concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in ASC alone, while lower concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in VSC only. Real-time observation by confocal microscopy showed that ROS production by VSC under the condition of co-culture, which showed a notable increase at 15 min, was significant less than that in independent culture, which peaked at 5 min and was gradually decreased. ROS production by ASC in co-culture began to increase significantly at 10 min. CONCLUSION: Compared to independent culture, ASC reduces the resting ROS production by co-cultured with VSC, while ASC prolongs the duration of ROS production by VSC after exposed to excitatory stimulus.     

冀北山地华北落叶松人工林对降水截留效应的初步研究

在冀北山地华北落叶松集中分布的地区,利用对降水在林分中的样地进行定位观测的方法,研究了该地区主要造林树种华北落叶松对降水的截留效应。据2002-2005年的连续观测,结果表明:华北落叶松林冠平均截留率为23.6%,树干茎流率占大气降水量的3.7%,枯枝落叶层的截留率为10.2%。说明华北落叶松在冀北山地发挥了巨大的水文生态作用。