高压电场在食品物料干燥中的应用研究

高压电场干燥是一种全新的干燥技术。文章分析了高压电场干燥的机理，认为是“不均匀高压电场产生的离子风的冲击作用”．而不是“传热传质”。介绍了高压电场干燥的发展历程及进展，指出了高压电场干燥的发展潜力和前景，并提出了设想。     

泵站机组不能正常启动原因分析及解决办法

针对江苏省淮安第三抽水站机组不能正常启动的问题进行了分析，找出了原因所在，通过对阻尼环等的技术改进，解决了机组存在的质量问题。指出了泵站工程建设中必须重视工程每个细小构件的设计，研究施工工艺，严格控制产品质量，确保新建工程优质、安全、高效。     

电力系统日有功优化调度的工程化软件与应用

随着电力市场的逐步建立和电力供需形势的变化，电力系统的优化调度具备了可行条件；同时，由于电力部门运作带有的计划性，需要开发出实用有效的、涉及多种人为调控约束的工程化方法用于实际生产。针对上述需要，建立了考虑若干为调控约束的日有功优化调度工程化模型，提出了基于专家规则、优先顺序和动态规划法的工程化算法，并开发了相应的工程化软件。该软件已在陕西电网成功运行一年，取得了显著的效益。     

Effects of naringenin on PI3K/AKT signaling pathway and endoplasmic reticulum stress and its related apoptotic pathways in rats with myocardial ischemia/reperfusion injury

AIM To observe the effect of naringenin on cardiac injury in ischemia/reperfusion （I/R） rats, and to explore whether the role of naringenin is involved in PI3K/AKT signaling pathway and endoplasmic reticulum stress and its related apoptotic pathways. METHODS SD rats （n=48） were randomly divided into sham operation （sham） group, model （I/R） group, naringenin treatment （NAR） group and naringenin+LY294002 （NL） group. Myocardial I/R injury model was prepared by ligation of left anterior descending coronary artery of rats for 30 min followed by reperfusion for 120 min. After reperfusion, the serum levels of cardiac troponin I （cTnI） was measured by ELISA. HE staining, TTC staining and TUNEL staining were used to detect the myocardial histopathological changes, myocardial infarction area and myocardial cell apoptotic rate. The mRNA levels of endoplasmic reticulum stress-related indicators glucose-regulated protein 78 （GRP78）, C/EBP homologous protein （CHOP） and caspase-12 were detected by RT-qPCR. The protein levels of cleaved caspase-3, GRP78, CHOP, caspase-12, p-PI3K and p-AKT were determined by Western blot. RESULTS Compared with I/R group, the serum content of cTnI, myocardial pathological damage, myocardial infarction area and myocardial cell apoptotic rate were significantly reduced （P<0.05）, the protein levels of cleaved caspase-3, GRP78, CHOP and caspase-12 were decreased （P<0.05）, and the protein levels of p-PI3K and p-AKT were increased in NAR group （P<0.05）. LY294002 attenuated the protective effect of naringenin to some extent. CONCLUSION Naringenin reduces myocardial I/R injury in rats possibly by activating PI3K/AKT signaling pathway and subsequently regulating endoplasmic reticulum stress and its related apoptotic pathways.     

农村养蚕小窍门

笔者已多年从事蚕桑生产,深入蚕户,积累了许多有用的经验,供广大蚕农借鉴。1“防尘口罩”的制作很多蚕农都知道,杀灭病原,严防蚕病发生,关键是消毒严格、彻底。但在蚕体蚕座使用防僵粉或鲜石灰粉时,灰尘很大,气味刺鼻,所以常常草草了事。要使药物撒周到,很简单,就是把平常用的口罩用水打湿后再用,粉粒就会粘附在口罩上,并且气味也减小了。2经济、实用的熟蚕自动上蔟登蔟剂现有很多蚕农推广熟蚕自动上蔟,这样可以节省劳力和时间。目前农村养蚕用于熟蚕自动上蔟的登蔟剂最常用的有两种,即樟脑丸和鲜腥草(俗名侧耳根),它们都有一种特殊的气味,…     

上杭湖洋当前茶叶采摘上的问题及对策

本文通过对湖洋乡主要茶场的调查分析。对当前茶叶采摘存在的主要问题。提出解决问题的建议和具体措施：一是茶叶采摘上搞好分期分批采茶。严格采摘标准。加强配套措施,推广采茶机具;二是经营管理上做好技术培训与指导,完善承包责任制。健全茶叶验收制度和使用洁净用具等。     

A Novel Gelatinase from Marine Flocculibacter collagenilyticus SM1988: Characterization and Potential Application in Collagen Oligopeptide-Rich Hydrolysate Preparation

Although the S8 family in the MEROPS database contains many peptidases, only a few S8 peptidases have been applied in the preparation of bioactive oligopeptides. Bovine bone collagen is a good source for preparing collagen oligopeptides, but has been so far rarely applied in collagen peptide preparation. Here, we characterized a novel S8 gelatinase, Aa2_1884, from marine bacterium Flocculibacter collagenilyticus SM1988^T, and evaluated its potential application in the preparation of collagen oligopeptides from bovine bone collagen. Aa2_1884 is a multimodular S8 peptidase with a distinct domain architecture from other reported peptidases. The recombinant Aa2_1884 over-expressed in Escherichia coli showed high activity toward gelatin and denatured collagens, but no activity toward natural collagens, indicating that Aa2_1884 is a gelatinase. To evaluate the potential of Aa2_1884 in the preparation of collagen oligopeptides from bovine bone collagen, three enzymatic hydrolysis parameters, hydrolysis temperature, hydrolysis time and enzyme-substrate ratio (E/S), were optimized by single factor experiments, and the optimal hydrolysis conditions were determined to be reaction at 60 ℃ for 3 h with an E/S of 400 U/g. Under these conditions, the hydrolysis efficiency of bovine bone collagen by Aa2_1884 reached 95.3%. The resultant hydrolysate contained 97.8% peptides, in which peptides with a molecular weight lower than 1000 Da and 500 Da accounted for 55.1% and 39.5%, respectively, indicating that the hydrolysate was rich in oligopeptides. These results indicate that Aa2_1884 likely has a promising potential application in the preparation of collagen oligopeptide-rich hydrolysate from bovine bone collagen, which may provide a feasible way for the high-value utilization of bovine bone collagen.     

粮食安全视域下生态低碳农业的发展战略与路径选择

实现粮食安全战略与“双碳”目标是加快农业农村现代化步伐、加快建设农业强国的关键。为助推粮食安全战略与“双碳”目标的同步实现,本研究立足粮食安全视域,阐释生态低碳农业的科学内涵,厘清粮食安全与生态低碳农业的辩证关系,剖析粮食安全战略下生态低碳农业发展面临的问题与挑战,并针对性提出发展战略与推进路径。研究发现,在“大食物观”“大产业观”“大农业观”指导下,生态低碳农业涉及全食物品类、全产业流程、全生活环节。粮食安全是发展生态低碳农业的底线要求,生态低碳农业是可持续保障粮食安全的应有之义。当前,发展生态低碳农业面临自然资源约束、科学技术瓶颈、小农生产模式、居民观念局限等现实困难,亟需探索水地资源拓展、技术创新应用、居民素质提升等有效路径,推动生态低碳农业实现“藏粮于地”“藏粮于技”“藏粮于民”。     

新型保铃剂防治棉花烂铃的效应

应用新型保铃剂进行了防治棉花烂铃的效果试验.结果表明,在棉花生长盛期,用新型保铃剂进行多次叶面喷施,结合科学栽培管理措施,可以起到防治烂铃、增加产量的效果,与不用保铃荆的处理相比,烂铃减少13%,产量增加16%;在每公顷密度15 000株、氮肥用量450 kg的情况下,配合喷施新型保铃剂的互作效应最好,烂铃最多减少42%,产量最多增加17%.     

葡萄糖氧化酶基因植物表达载体的构建

为进一步开展转基因研究创造条件,用限制性内切酶将葡萄糖氧化酶(GOD)基因从pMD/GO载体上切下,定向连接到植物表达载体pROKⅡ的CaMV35S启动子下游和NOS终止子上游,成功地构建了GOD基因植物表达载体pROK/GO。利用冻融法将此表达载体导入根癌农杆菌LBA4404中,提取转化质粒,经PCR和酶切鉴定表明,GOD基因植物表达双元载体构建成功。