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171.
AIM:To observe whether selective inhibition of endothelin receptor A (ETRA) improves white matter lesions (WMLs), and explore the mechanism. METHODS:Sprague-Dawley rats (n=33) were randomly divided into sham operation group (n=9), treatment group[stroke-prone renovascular hypertensive rats-modified 2 vessel occlusion (RHRSP-modified 2VO) + ambrisentan (n=12)] and placebo group[RHRSP-modified 2VO + vehicle (n=12)]. Drug and vehicle administration was performed from 17th to 20th week and monitoring of systolic arterial pressure was performed weekly. Morris water maze test was conducted to evaluate the function of cognition. The protein levels of endothelin-1 (ET-1) in the cortex, corpus callosum and caudate putamen were quantitatively analyzed respectively. The severity of WMLs and the relationship between ET-1 and vessels were observed by the method of histopathology. RESULTS:The difference of systolic arterial pressure between treatment group and placebo group was not significant. The animals in treatment group exhibited shorter escape latency (P<0.05), more times of crossing platform (P<0.05), lower level of ET-1 in corpus callosum and caudate putamen (P<0.05), respectively, improved WMLs severity (P<0.05) and lower binding level of ET-1 to vessels compared with the placebo group. CONCLUSION:Selective inhibition of endothelin receptor A improves the severity of WMLs and ameliorates the cognitive function. 相似文献
172.
黄瓜的高温胁迫伤害及其耐热性研究进展 总被引:5,自引:0,他引:5
高温胁迫是设施蔬菜栽培不可避免的一种逆境因子。本文综合阐述了高温胁迫对黄瓜营养生长和生理生化、生殖生长以及产量和品质等方面的不良影响,介绍了黄瓜耐热性遗传规律并总结了当前主要的耐热性鉴定方法和提高耐热性的技术措施,并展望了今后进一步深入研究黄瓜耐热性的方向。 相似文献
173.
陕北黄土高原生态资产与区域生态安全程度研究 总被引:1,自引:1,他引:1
在GIS支持下,通过测定陕北黄土高原植被生产和生态调节的物质量及生态资产价值.结果表明:①通过计算,陕北黄土高原每年的生态资产总价值为7.00×1010元,其中释放氧气的价值最高,占总价值的29.51%;土壤保持价值最小,占总价值的1.5%.而就植被类型而言,果树的生态价值最高,占总价值的43.89%;最小是沼泽,占总价值的0.4%.②通过地理信息系统与生态学的结合,对陕北黄土高原的生态资产进行定量测量,并利用GIS软件的空间分析功能,绘制了陕北黄土高原生态资产价值空间分布图.③陕北黄土高原生态安全程度为0.45,处于生态不安全状态,负荷超载,要狠抓生态建设.④针对陕北黄土高原环境现状,提出相应的治理对策. 相似文献
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ZHANG Han MA Jing ZHANG Yun-ling ZHANG Shu-ming XU Qing-rui WANG Wei-ming 《园艺学报》2015,31(12):2244-2248
AIM: To investigate whether Mycoplasma pneumoniae (Mp)-induced interleukin-1β (IL-1β) production in RAW264.7 cells is through the activation of NLRP3 inflammasome via reactive oxygen species (ROS). ME-THODS: RAW264.7 cells were randomly divided into 3 groups. In normal group, RAW264.7 cells were treated without Mp. In model group, RAW264.7 cells were treated with 1∶ 10 multiplicity of infection (MOI) of Mp. In NAC group, RAW264.7 cells were pretreated with N- acetylcysteine (NAC) at a concentration of 5 mmol/L for 30 min before infection with Mp. The RAW264.7cells were infected with Mp (1∶ 10 MOI) for 4, 8, 16 and 24 h in model group and NAC group, respectively. The intracellular ROS level was analyzed by flow cytometry. The mRNA expressions of NLRP3, ASC and caspase-1 were detected by real-time PCR. The protein levels of NLRP3, ASC and caspase-1 p20 were determined by Western blot. The levels of pro-inflammatory cytokine IL-1β in the supernatant were measured by ELISA. RESULTS: Compared with normal group, the production of ROS were significantly increased at 4, 8, 16 and 24 h after infection, the mRNA expression of NLRP3, ASC and caspase-1 were increased at 8, 16 and 24 h after infection, the protein levels of NLRP3, ASC and caspase-1 p20 were increased at 16 and 24 h after infection, and the releases of IL-1β were increased at 24 h after infection in model group (P<0.01). Compared with the model group, the level of ROS in NAC group decreased, so as the expression of NLRP3, ASC and caspase-1 at mRNA and protein levels and the releases of IL-1β in the supernatant at the corresponding time points. CONCLUSION: Mp may stimulate the ROS production to activate NLRP3 inflammasome in RAW264.7 cells. 相似文献
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WU Xia HUANG Wan-yi HAN Sha-sha SUN Feng-jie XU Jing YANG Xue-song LIU Guo-sheng 《园艺学报》2014,30(10):1765-1771