A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

To tackle the growing problem of antibiotic resistance, it is essential to identify new bioactive compounds that are effective against resistant microbes and safe to use. Natural products and their derivatives are, and will continue to be, an important source of these molecules. Sea sponges harbour a diverse microbiome that co-exists with the sponge, and these bacterial communities produce a rich array of bioactive metabolites for protection and resource competition. For these reasons, the sponge microbiota constitutes a potential source of clinically relevant natural products. To date, efforts in bioprospecting for these compounds have focused predominantly on sponge specimens isolated from shallow water, with much still to be learned about samples from the deep sea. Here we report the isolation of a new Micromonospora strain, designated 28ISP2-46^T, recovered from the microbiome of a mid-Atlantic deep-sea sponge. Whole-genome sequencing reveals the capacity of this bacterium to produce a diverse array of natural products, including kosinostatin and isoquinocycline B, which exhibit both antibiotic and antitumour properties. Both compounds were isolated from 28ISP2-46^T fermentation broths and were found to be effective against a plethora of multidrug-resistant clinical isolates. This study suggests that the marine production of isoquinocyclines may be more widespread than previously supposed and demonstrates the value of targeting the deep-sea sponge microbiome as a source of novel microbial life with exploitable biosynthetic potential.     

In vitro activity of isothiocyanates against Fusarium graminearum

Isothiocyanates are biotoxic degradation products formed as a result of enzymatic hydrolysis of glucosinolates present in Brassica species. The application of biofumigant Brassica crops, as an alternative crop protection method for soilborne pathogens and pests is increasingly gaining interest. However, little is known of the potential of biofumigation to reduce the inoculum of Fusarium species affecting cereals. The aim of this study was to evaluate the antifungal activity of five isothiocyanates, namely allyl, benzyl, ethyl, 2-phenylethyl and methyl isothiocyanates, against germination and growth of Fusarium graminearum under in vitro conditions. Aromatic isothiocyanates were more inhibitory than the aliphatic isothiocyanates against mycelial growth, whereas the reverse was observed for conidial germination. Among the tested isothiocyanates, allyl and methyl isothiocyanates were more efficient overall, showing lower ED₅₀ values (35–150 mg/L) for conidial germination and mycelial radial growth. The findings suggest that Brassica plants containing allyl and methyl glucosinolates could have a suppressive effect, reducing the inoculum of F. graminearum in soil prior to cereal production.     

Endosperm-Specific Expression of Serotonin <Emphasis Type="Italic">N</Emphasis>-Hydroxycinnamoyltransferase in Rice

Serotonin N-hydroxycinnamoyltransferase (SHT) is a key enzyme in the synthesis of feruloylserotonin (FS) and 4-coumaroylserotonin (CS). These serotonin derivatives show strong antioxidant activity, making them valuable for both nutritional and pharmacological use in humans. Ectopic expression of SHT under the control of the endosperm specific-glutelin and prolamin promoters from rice was produced via Agrobacterium-mediated transformation. SHT expression was confirmed by Southern blot analysis, followed by Northern blotting and SHT enzyme activity analyses using total RNA and protein, respectively, extracted from transgenic seeds. The glutelin A3 (GluA3) promoter produced low SHT mRNA expression in rice seeds, whereas the prolamin promoter expressed high levels of SHT mRNA. In spite of the ectopic expression of SHT in rice seeds, both transgenic genotypes accumulated levels of serotonin derivatives similar to those found in wild-type rice. Furthermore, our data suggest that serotonin, rather than phenylpropanid-CoAs, is the rate-limiting substrate in the biosynthesis of serotonin derivatives in SHT-overexpressing transgenic rice seeds.     

Radiographic evaluation of nonanesthetized and nonsedated dogs for hip dysplasia

The use of chemical or gas restraint was unnecessary in most large breed dogs being evaluated radiographically for hip dysplasia. Of 100 large-breed dogs, 97 were successfully radiographed for hip dysplasia evaluation without the use of sedation or anesthesia.     

Wedge and Eggbar Shoes Change the Pressure Distribution Under the Hoof of the Forelimb in the Square Standing Horse

The shoe types most commonly applied to horses with navicular disease or other forms of palmar heel pain are shoes with heel wedges and eggbar shoes, although their efficacy has been a matter of debate among veterinarians and farriers for centuries. To quantify the effect of these different types of “navicular” shoeing on static hoof pressure distribution, 6 warmblood horses were shod with 6° wedge, eggbar, and plain shoes. While standing square with weight evenly distributed across both forelimbs, the center of pressure and pressures at selected areas of interest (AOI: toe, medial and lateral toe, medial and lateral heel) were measured using a Footscan (RsScan International, Belgium) pressure plate in a Latin square design using the plain shoe as a reference.Wedge shoes did not provide a significant shift in the center of pressure. The application of eggbar shoes did not alter the relative position of the center of pressure under the hoof. However, the absolute distance from the toe to the center of pressure was significantly larger with eggbar shoes (77 + 12 mm) compared with plain and wedged shoes (70 ± 8 mm, P < .05) resulting in an absolute, caudal shift of the center of pressure. When pressure (N/cm²) values at the five AOIs were averaged for each shoe type, the wedge and eggbar shoe recordings showed a significantly lower mean pressure than plain shoes (P < .05).In conclusion, mean AOI pressures decreased with wedge and eggbar shoes, and eggbar shoes provided a caudal shift in the center of pressure. These effects are believed to decrease the moment of the coffin joint and reduce the pressure on the navicular bone. Thus, the findings of this study might contribute to the scientific evidence of efficacy of the use of wedge and eggbar shoes in “navicular” lame horses.     

Osteochondral dysplasia of the coxofemoral joints in a Friesian foal: Clinical findings and methods of diagnosis

Diseases of the coxofemoral (hip) joint are infrequently diagnosed in horses. Most cases are presented as an unilateral condition and usually are of traumatic origin. This case report describes a Friesian foal with a clinically obvious thoracolumbar kyphosis, combined with a weight‐shifting stance and a shortened stride of both hindlimbs. General clinical and lameness examinations, computed tomographic examination of the pelvis, and macroscopic and histopathological examinations of the coxofemoral joints were performed. This revealed a final, phenotypical diagnosis of a primary osteochondral dysplasia of both coxofemoral joints with secondary osteoarthritis. Similar to the occurrence of this condition in other species and considering the small genetic basis of the Friesian horse breed, a genetic predisposing factor is suspected to play a key role in the developing mechanism of dysplastic coxofemoral joint disease in horses as illustrated with this case. Computed tomography scanning appears to be a useful imaging technique in the detection of coxofemoral joint disease in small horses and foals.