A qualitative colorimetric test for brominated vegetable oil in soft drinks

A simple and precise method of detecting brominated vegetable oil (BVO) in soft drinks is described. After extraction of BVO using diethyl ether, the concentrated ethereal solution was treated with a small quantity of zinc dust to convert the organic bromide to inorganic form; the solution was subsequently treated with lead dioxide to liberate bromine. The bromine evolved was detected by means of fluorescein-impregnated filter paper strip that turns pink because eosin is formed. The test can detect as low as 10 ppm (2 mg/200 ml) of BVO under experimental conditions. Gas chromatography was carried out on sodium methoxide derivatives prepared from ether extract for quantitation.     

Molecular properties as descriptors of octanol-water partition coefficients of herbicides

Molecular modeling techniques were used to establish relationships between the octanol/water partition coefficient (K _ow) and molecular properties of 90 herbicides with several types of chemistry. The K_ow values were obtained from the literature. Various molecular properties were calculated by quantum mechanical methods using molecular modeling software. The quantitative structure activity relationship (QSAR) analysis of all herbicides showed that K _ow was dependent on bulk (van der Waals volume, VDW_v) and electronic (dipole moment, µ; superdelocalizability of highest occupied molecular orbital, SHOMO; nucleophilic superdelocalizability, SN) properties, and the model explained 68% of the variation in K _ow. Herbicides were broadly divided into six families (ureas, acid amides, triazines, carbamates, diphenyl ethers, and dinitroanilines) based on structural similarities, and separate equations were established for each group. The QSAR models accounted for 74 to 98% of the variation in K _ow within these six groups. Applicability of these models was tested for some herbicides. The QSAR models produced estimates that correlate well with experimental values and appear to be specific to structurally similar compounds.     

Aminomethylphosphonic acid, a metabolite of glyphosate, causes injury in glyphosate-treated, glyphosate-resistant soybean

Glyphosate-resistant (GR) soybean [Glycine max (L.) Merr.] was developed by stable integration of a foreign gene that codes insensitive enzyme 5-enolpyruvylshikimate-3-phosphate synthase, an enzyme in the shikimate pathway, the target pathway of glyphosate. Application of glyphosate to GR soybean results in injury under certain conditions. It was hypothesized that if GR soybean is completely resistant to the glyphosate, injury could be caused by a metabolite of glyphosate, aminomethylphosphonic acid (AMPA), a known phytotoxin. Glyphosate and AMPA effects on one- to two-trifoliolate leaf stage (16-18-days old) GR and non-GR soybean were examined in the greenhouse. In GR soybean, a single application of glyphosate-isopropylammonium (1.12-13.44 kg/ha) with 0.5% Tween 20 did not significantly reduce the chlorophyll content of the second trifoliolate leaf at 7 days after treatment (DAT) or the shoot dry weight at 14 DAT compared with Tween 20 alone. A single application of AMPA (0.12-8.0 kg/ha) with 0.5% Tween 20 reduced the chlorophyll content of the second trifoliolate leaf by 0-52% at 4 DAT and reduced shoot fresh weight by 0-42% at 14 DAT in both GR and non-GR soybeans compared with Tween 20 alone. AMPA at 0.12 and 0.50 kg/ha produced injury in GR and non-GR soybean, respectively, similar to that caused by glyphosate-isopropylammonium at 13.44 kg/ha in GR soybean. AMPA levels found in AMPA-treated soybean of both types and in glyphosate-treated GR soybean correlated similarly with phytotoxicity. These results suggest that soybean injury to GR soybean from glyphosate is due to AMPA formed from glyphosate degradation.     

Linking physical, economic and institutional constraints of land use change and forest conservation in the hills of Nepal

This paper analyses the changes in spatial patterns of agricultural land use during the period 1976–2000 along the altitudinal gradients in a watershed in Nepal. Land change patterns during this period were examined using information on land use derived from satellite images from 1976, 1990 and 2000. During the 24-year period from 1976 to 2000 agricultural land use increased by 35% at a cost of loss of forestland. Agricultural expansion was most conspicuous at higher elevations (1150–2000-m). About 36%, 18% and 6% of forestland was converted into agricultural activities from higher, middle and lower elevations respectively in the period from 1990 to 2000. Spatial distribution of living standard parameters, including farm family income and, food availability obtained from family surveys, shows a decreasing trend as the elevation increases whereas percentage of food bought shows an increasing trend. In this way it was found that, lost forest areas were smaller when located around high-income areas with good quality agricultural land and near an administrative centre as compared to areas located around low-income areas with low quality agricultural land and far from an administrative centre. Additionally, a regression model is constructed, to link the socioeconomic variables with the conversion of forestland into agricultural activities, breaking the study area into smaller zones. The spatial trajectories of these zones are then contrasted, paying particular attention to the socioeconomic conditions and institutional arrangements governing access to land resources. The study finds that while overall land change patterns in the region are largely explained by elevation and the socioeconomic conditions of people living adjacent to the forestland, more specifically, in sub-regional areas, trajectories reflect the signatures of institutions governing access to land. As sustainability of the watershed is dependent on forests, continued depletion of forest resources will result in poor economic returns from agriculture for local people together with loss of ecosystem services.     

Variation in the reproductive performance ofEpiricania melanoleuca (Fletcher) (Lep., Epipyropidae) in relation to stage and sex of the hostPyrilla perpusilla (Walker) (Hem., Lophopidae) during rearing

Rearing ofEpiricania melanoleuca (Fletcher) on adult females ofPyrilla perpusilla (Walker) augmented the productivity of the former, which is an effective nymphal and adult ectoparasitoid of the latter, in terms of yield of total and viable eggs. The applied value of this finding in field biocontrol program is highlighted.

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Zusammenfassung Wurden die ektoparasitischen Raupen vonEpiricania melanoleuca auf adulten Weibchen der Zuckerrohr-Zikade,Pyrilla perpusilla aufgezogen, vergrößerte sich die Zahl und Lebensfähigkeit der Eier, die von den daraus resultierenden Faltern abgelegt wurden. Das Ergebnis wird im Hinblick auf einen Einsatz des Parasitoiden zur biologischen Bekämpfung des Schädlings erörtert.

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With one table     

Oocyte-specific deletion of Pten causes premature activation of the primordial follicle pool

In the mammalian ovary, progressive activation of primordial follicles from the dormant pool serves as the source of fertilizable ova. Menopause, or the end of female reproductive life, occurs when the primordial follicle pool is exhausted. However, the molecular mechanisms underlying follicle activation are poorly understood. We provide genetic evidence that in mice lacking PTEN (phosphatase and tensin homolog deleted on chromosome 10) in oocytes, a major negative regulator of phosphatidylinositol 3-kinase (PI3K), the entire primordial follicle pool becomes activated. Subsequently, all primordial follicles become depleted in early adulthood, causing premature ovarian failure (POF). Our results show that the mammalian oocyte serves as the headquarters of programming of follicle activation and that the oocyte PTEN-PI3K pathway governs follicle activation through control of initiation of oocyte growth.     

Foliar washoff potential and simulated surface runoff losses of trifloxysulfuron in cotton

The surface runoff potential of trifloxysulfuron {N-[(4,6-dimethoxy-2-pyrimidinyl)carbamoyl]-3-(2,2,2-trifluoroethoy)-pyridin-2-sulfonamide sodium salt} in cotton (Gossypium hirsutum L.) production systems has not been evaluated. The objectives of this study were to (i) determine sorption/desorption coefficients for trifloxysulfuron; (ii) quantify foliar washoff of trifloxysulfuron when applied to cotton at the five-leaf stage; and (iii) determine the surface runoff potential of trifloxysulfuron when applied to cotton at the five-leaf stage and to bare soil. Freundlich sorption and desorption coefficients were 1.15 and 1.22, respectively. Sorption data indicated that trifloxysulfuron was moderately sorbed to soil and that it will be transported primarily in the dissolved phase of surface runoff. Foliar washoff studies revealed that approximately 91% of trifloxysulfuron applied to cotton at the five-leaf stage was available for washoff 72 h after application. Simulated rainfall (7.5 cm h-1) applied 1 day after herbicide application (7.9 g ha-1) resulted in average concentrations of trifloxysulfuron in surface runoff water of 0.8 microg L-1 for bare plots and 1.3 microg L-1 for cotton plots. Cumulative trifloxysulfuron losses in surface runoff from bare plots and cotton plots were 0.13 and 0.21 g ha-1, respectively. These values correspond to fractional losses of 1.7% for bare plots and 2.7% for cotton plots. Greater runoff losses of trifloxysulfuron from cotton plots were attributed to foliar washoff. Trifloxysulfuron runoff losses may be curtailed if the herbicide is applied early postemergence when canopy coverage is minimal, thereby reducing the potential for foliar washoff.     

Field evaluation of entomofungal pathogens against cowpea aphid,Aphis craccivora Koch,and their effect on two coccinellid predators

The present study was carried out to assess the field efficacy of 10 isolates of entomofungal pathogens on management of cowpea aphid, Aphis craccivora. All the tested isolates showed suppressive effect on aphid population. Two-year pooled data revealed lower incidence of 19.37–40.81 aphids/plant in the entomofungal pathogen treated plots compared to higher incidence of 88.07 aphids/plant in untreated control. Among the 10 isolates tested, Vl-8 isolate of Lecanicillium lecanii, Ma-6 isolate of Metarhizium anisopliae and Bb-5a isolate of Beauveria bassiana showed 78.01%, 77.42% and 76.91% reduction of aphid population, respectively, over control and were on par with each other. Significantly higher yields were recorded in the plots treated with Bb-5a, Ma-6 and Vl-8 isolates (1252, 1225 and 1208 kg/ha, respectively) compared to untreated control (916 kg/ha). The predatory coccinellid populations of Cheilomenes sexmaculata and Micromus timidus in entomofungal treated and untreated control plots were almost similar and found statistically non-significant, indicating the safety of fungal pathogens to these coccinellid predators.     

An approach to land-use planning in a hilly watershed using geographical information system

The eco-socio-economic balance of a region can be preserved by proper land-use planning, particularly in hilly watersheds where erosion caused by rainfall is an ever-present threat. This paper describes a methodology developed to compile a treatment-oriented land-use planning scheme for a hilly watershed using a geographical information system (GIS). A remote sensing based physiographic soils map and a digital elevation model (DEM) are the sources of soil depth and slope steepness classes, respectively, and are the information databases for GIS analysis. The GIS was used to integrate these databases and provide tools for manipulation of the data. A set of knowledge-based rules was formed from the classification scheme formulated by Sheng and Stennett (1975) for hilly watersheds, modified to allow for the local land-use patterns. These rules were used to manipulate the databases and allow the establishment of a sustained land-use system in the watershed with proper soil and water conservation measures. This biological engineering scheme would be a safeguard against soil erosion and other environmental threats. A priority sub-watersheds delineation survey conducted in the watershed indicates significant variation in the silt yield index values, which calls for conservation planning in earnest in cases of high and very high priority sub-watersheds.