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21.
Chi-Heung Cho Chang-Jun Lee Min-Gyeong Kim Bomi Ryu Jun-Geon Je Yoonsook Kim Sang-Hoon Lee 《Marine drugs》2022,20(6)
Advanced glycation end-products (AGEs) play a vital role in the pathogenesis of diabetic complications. Methylglyoxal (MGO), one of the major precursors of AGEs, is a highly reactive dicarbonyl compound that plays an important role in the pathogenesis of diabetic nephropathy. This study was designed to evaluate the therapeutic potential of phlorotannin-rich Ecklonia cava extract (ECE) on MGO-induced diabetic nephropathy in in vitro models using mouse glomerular mesangial cells. ECE showed anti-glycation activity via breaking of AGEs-collagen cross-links and inhibition of AGEs formation and AGE-collagen cross-linking formation. The renoprotective effects were determined by assessing intracellular reactive oxygen species (ROS) and MGO accumulation, cell apoptosis, and the Nrf-2/ARE signaling pathway. MGO-induced renal damage, intracellular ROS production level, and MGO-protein adduct accumulation were significantly decreased by pretreating ECE. Moreover, ECE pretreatment exhibited preventive properties against MGO-induced dicarbonyl stress via activation of the Nrf2/ARE signaling pathway and reduction of RAGE protein expression in mouse glomerular mesangial cells. Collectively, these results indicated potential anti-glycation properties and prominent preventive effects of ECE against MGO-induced renal damage. Additionally, ECE may be utilized for the management of AGE-related diabetic nephropathy. 相似文献
22.
Hyo-Geun Lee Hyun-Soo Kim Hyesuck An Kyunghwa Baek Jeong Min Lee Mi-Jin Yim Seok-Chun Ko Ji-Yul Kim Gun-Woo Oh Jun-Geon Je Dae-Sung Lee You-Jin Jeon 《Marine drugs》2022,20(6)
The aim of this study was to assess the potential hypertensive effects of the IGTGIPGIW peptide purified from Hippocampus abdominalis alcalase hydrolysate (HA) for application in the functional food industry. We investigated the antihypertensive effects of IGTGIPGIW in vitro by assessing nitric oxide production in EA.hy926 endothelial cells, which is a major factor affecting vasorelaxation. The potential vasorelaxation effect was evaluated using 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate, a fluorescent stain. IGTGIPGIW significantly increased the expression of endothelial-derived relaxing factors, including endothelial nitric oxide synthase and protein kinase B, in EA.hy926 cells. Furthermore, oral administration of IGTGIPGIW significantly lowered the systolic blood pressure (183.60 ± 1.34 mmHg) and rapidly recovered the diastolic blood pressure (143.50 ± 5.55 mmHg) in the spontaneously hypertensive rat model in vivo. Our results demonstrate the antihypertensive activity of the IGTGIPGIW peptide purified from H. abdominalis and indicate its suitability for application in the functional food industry. 相似文献
23.
Huimin Yang Murray Unkovich Ann McNeill Xianzhi Wang 《Biology and Fertility of Soils》2011,47(4):377-385
Symbiotic N2 fixation by lucerne (Medicago sativa) has capacity to provide significant inputs of N to agro-ecosystems, and the species has also been shown to scavenge soil
mineral N and thus act as a sink for excess reactive N. The balance between these two N cycle processes was investigated in
an extensive irrigated lucerne growing region where nitrate contamination of groundwater has been reported. We sampled 18
permanent pure lucerne stands under irrigation for standing dry matter, total shoot N, and N2 fixation using 15N natural abundance along with activity of the inducible enzyme nitrate reductase as indicators of use of soil NO3− by lucerne. On average 65% of lucerne N was obtained from symbiotic N2 fixation. Converting standing dry matter estimates to annual N2 fixation amounts we calculated average N2 fixation of 311 kg N/ha, including N in roots and nodules. Uptake of N from soil by lucerne was calculated to be 181 kg N/ha/year.
We were not able to identify the source of this soil mineral N, although nitrate reductase activity of lucerne was higher
than that of non-N2 fixing species examined. 相似文献
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Chang‐Hoon Shin Hien Thi Dieu Bui Samad Rahimnejad Ji‐Hoon Cha Byung‐Woo Yoo Bo‐Kyeun Lee Hyung‐Jin Ahn Soo‐Il Choi Yun‐Jeong Choi Yong‐Ho Park Jeong‐Dae Kim Kyeong‐Jun Lee 《Journal of the World Aquaculture Society》2014,45(3):258-268
This study was conducted to evaluate the effects of dietary supplementation of Barodon, an anionic alkali mineral complex, on growth, feed utilization, humoral innate immunity and disease resistance of olive flounder. A basal experimental diet was used as a control and supplemented with 0.1, 0.2, 0.3, 0.4, or 0.5% Barodon. Triplicate groups of fish (26.4 ± 0.2 g) were fed one of the diets to apparent satiation twice daily for 10 wk. The growth performance was enhanced (P < 0.05) linearly and quadratically in fish fed diets containing Barodon compared with that in fish fed the control. Feed utilization was significantly improved by Barodon supplementation. Serum lysozyme and antiprotease activities were increased quadratically in Barodon fed groups. Also, significantly higher superoxide dismutase activity was found in Barodon‐fed fish. Dietary supplementation of 0.1–0.3% Barodon resulted in significant enhancement of fish disease resistance against Streptococcus iniae. The findings in this study indicate that dietary supplementation of Barodon can enhance growth, feed utilization, innate immunity, and disease resistance of olive flounder and that the optimum level seems to be 0.1% in diets. 相似文献
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The effects of ammonia and nitrite on vigour, survival rate, moulting rate of zoea of blue swimming crab, Portunus pelagicus, were studied. A total of five nitrite-N treatments (26.67, 53.34, 106.68, 213.36, 426.72 mg/l) and a control (no nitrite-N added) were
set up for the acute nitrite-N toxicity experiment; a total of five ammonia-N treatments (8.43, 16.86, 33.72, 67.44, 134.88 mg/l)
and a control (no ammonia-N added) were set up for the acute ammonia-N toxicity experiment. The results showed that the vigour,
survival rate and moulting rate of zoea of the blue swimming crabs exposed to over 53.34 mg/l were significantly different
(P < 0.05) from the control group. The zoea LC50 values (mg/l) of nitrite-N were 179.47, 76.56, 66.70, 37.49, 25.01, 25.35, 25.34 mg/l for 24, 36, 48, 60, 72, 84, 96 h, respectively.
The vigour, survival rate and moulting rate of zoea of the blue swimming crabs exposed to over 16.86 mg/l were significantly
different (P < 0.05) from the control group. The zoea LC50 values (mg/l) of ammonia-N were 51.04, 39.62, 38.72, 24.43, 16.90, 13.42, 11.16 mg/l for 24, 36, 48, 60, 72, 84, 96 h, respectively.
The zoeae are highly sensitive to ammonia and nitrite, and the toxicity of ammonia and nitrite on Portunus pelagicus decrease with development of this crab. 相似文献