Changes of cardiac function,RAGE expression and calcium dysregulation in type 2 diabetic rats

AIM: To investigate the changes of cardiac structure and function in rats with type 2 diabetic mellitus (T2DM), and to explore the mechanisms underlying diabetic cardiomyopathy. METHODS: The cardiac structure and function were measured by echocardiography in Zucker diabetic fatty (ZDF) rats and their control Zucker lean (ZL) rats. The size of the cardiomyocytes was determined by wheat germ agglutinin staining. The protein expression of atrial natriuretic peptide (ANP), β-myosin heavy chain (β-MHC), receptor for advanced glycation end products (RAGE), L-type cal-cium channel α1C subunit (Ca_V1.2) and Orai1 was assessed by Western blot. RESULTS: Compared with the ZL control rats, the thickness of left ventricular wall, ejection fraction (EF), fractional shortening (FS) and the sizes of cardiomyocytes were significantly increased, and diastolic function was decreased in the ZDF rats (P<0.05). The protein expression of β-MHC, ANP, RAGE and Orai1 was increased, while the expression of Ca_V1.2 was decreased in ZDF rats (P<0.05). CONCLUSION: T2DM rats show the prominent features including cardiomyocyte hypertrophy, ventricular hypertrophy and compensatory enhancement of cardiac function, and the Ca²⁺ handling and increase in RAGE expression may play important roles in the processes.     

红豆杉组织培养的防褐变措施研究

以红豆杉腋芽为外植体,B5为基本培养基,研究了不同激素、添加剂组合对红豆杉组织培养中褐变的影响。结果表明:添加激素2,4-D 2.0mg·L~(-1)+6-BA 0.5mg·L~(-1),愈伤组织生长情况最好,染菌率为11.11%,愈伤组织出愈率最高达88.89%,褐化程度较低,多酚氧化酶(PPO)活性为8.03U·g~(-1)·min~(-1);加入添加剂组合活性炭和柠檬酸,可以降低红豆杉的褐变程度,PPO活性为1.40U·g~(-1)·min~(-1)。     

番茄质地构成与贮藏期间主要性状的相关分析

以9个番茄品种为材料,测定采后果实质地参数及贮藏期间主要性状变化,分析其质地参数与贮藏期间主要性状之间的相关性。结果表明:硬度与可溶性固形物极显著负相关,与腐烂率显著负相关;凝聚性与胶性、咀嚼性极显著正相关,与粘弹性显著正相关;粘弹性与咀嚼性极显著正相关,与呼吸强度极显著负相关;胶性与咀嚼性极显著正相关,与腐烂率显著负相关。质构仪测定的硬度及胶性可以作为耐贮藏性的重要指标。     

全生物降解农用地膜对比试验

研究全生物降解农用地膜、聚乙烯和稻草覆盖3种不同处理对土壤含水量、土壤温度以及大顶苦瓜产量和经济效益的影响。试验结果表明,全生物降解农用地膜具有保温保水功能,而且能够达到预期降解效果,纯收入比较:全生物降解农用地膜聚乙烯地膜稻草覆盖,综合来看,K9-10全生物降解农用地膜在实践中使用效果较好。     

Hypermethylation of SFRP genes in esophageal squamous cell cancer

AIM: To investigate the promoter methylation of secreted frizzled-related protein(SFRP) genes in esophageal squamous cell cancer(ESCC). METHODS: The methods of methylation-specific PCR(MS-PCR) and RT-PCR were applied to examine the CpG methylation of the SFRP promoter and the mRNA expression of SFRP genes,respectively, in 78 samples of ESCC and corresponding adjacent non-cancer tissues. The protein expression of β-catenin was determined by immunohistochemistry.RESULTS: In the ESCC tissues, the frequencies of promoter methylation in SFRP1 , SFRP2 , SFRP4 and SFRP5 genes were 65.4%(51/78), 69.2%(54/78), 62.8%(49/78) and 52.6%(41/78),respectively, significantly higher than those in the adjacent tissues(P<0.01). The hypermethylation of these genes had no correlation with clinical stage and pathological classification in ESCC tissues(P>0.05). The frequency of simultaneous methylation of the 4 genes was correlated with the clinical stage(P<0.05). The positive rates of mRNA expression of the 4 genes in ESCC tissues were 42.3%(33/78), 46.2%(36/78), 50.0%(39/78) and 39.7%(31/78), respectively lower than those in the adjacent tissues(P<0.01). The mRNA expression of SFRP genes and the ectopic expression of β-catenin were correlated with the methylation frequency of SFRP genes(P<0.01).CONCLUSION: Promoter methylation of SFRP1 , SFRP2 , SFRP4 and SFRP5 genes was a frequent event in ESCC, indicating a contribution to the pathogenesis of ESCC through aberrant canonical Wnt/β-catenin signaling pathway. Combination analysis of methylation status in SFRP genes may has definite value on estimating prognosis of ESCC.     

香蕉枯萎病菌新毒素——白僵菌素的鉴定

 调查并分离了广东、海南、广西、福建和云南等香蕉枯萎病疫区的一批病原菌样本。利用高效液相色谱—电喷雾离子阱质谱（HPLC-ESI-MS）法对所分离的28个Fusarium oxysporum f. sp. cubense菌株的次生代谢产物进行初步分析。发现了分子量为179 D的镰刀菌酸及分子量约为783 D的未知产物;利用¹H-NMR对一株4号生理小种的未知产物进行了结构测定,结果表明该化合物为白僵菌素。离体试验表明白僵菌素能够导致香蕉假茎腐烂。     

Expression of IL-1β and IL-17 on mast cells in human periapical diseases

AIM：To identify and quantify the expression of IL-1β and IL-17 in mast cells (MCs) in different types of human pericapical diseases using double immunofluorescence staining. METHODS：The specimens (n=102), including healthy control (n=35), periapical cyst (n=35) and periapical granuloma (n=32), were involved in the present study. The tissue samples were fixed in 10 % buffered formalin for at least 48 h and then embedded in paraffin. Serial 5-μm-thick sections were deposited onto SuperFrost/Plus microscope glasses. Routine staining of the sections using hematoxylin & eosin (HE) was performed for morphological evaluation. The number of IL-1β and IL-17 positive MCs was identified by double immunofluorescence staining. RESULTS：Compared with the healthy controls, the inflammation score of periapical lesions was significantly increased in the periapical patients (P<0.01). The density of IL-1β and IL-17 positive MCs in the periapical lesions were obviously higher than that in the healthy controls (P<0.01). However, no significant difference between periapical cyst and periapical granuloma was observed. The Pearson correlation analysis showed that there was a positive correlation between the density of IL-1β and IL-17 double positive MCs and inflammation score in different groups of specimens (P<0.01). CONCLUSION：There is significantly increased number of MCs, along with increased density of IL-1β and IL-17 positive MCs in human periapical lesions. The increased density of IL-1β and IL-17 positive MCs has the similar tendency as the severity of tissue inflammation in human periapical lesions, suggesting that IL-1β and IL-17 positive MCs may play an important role in the pathogenesis of human periapical diseases.     

莲基因组学和分子生物学研究进展

综述了莲藕基因组学和分子生物学研究进展,主要包括莲核基因组和细胞器基因组测序、系统进化和遗传多样性、重要基因挖掘和功能分析等,并对其今后的研究进行了展望。     

催芽药剂对秋季脱毒马铃薯原原种繁育产量的影响

以催芽药剂、浸泡浓度及浸泡时间为因素设计了L^9（3^4）正交试验,研究打破秋繁马铃薯原原种休眠的最适处理方法。结果表明,以5 mg/L的赤霉素（GA3）浸泡10 min的处理产量最高,为724.93 kg/667m^2,比其他处理增产17.6%-114.9%;各因素对产量的决定程度由大到小排列依次为：催芽药剂（A）〉浸泡浓度（B）〉浸泡时间（C）。     

白浆土某些物理性质的研究Ⅲ.白浆土微结构及孔隙特征

为探究白浆土的某些物理性质,对自然林下的白浆土和耕地白浆土的土壤微结构进行对比分析。结果表明:自然林下荒地白浆土剖面未受到过任何人为扰动,表层土壤中有大量的植物根系和土壤动物的残留物质,土壤团粒结构发达,孔隙量大,土质疏松;表土层向白浆层呈渐进式过渡;而耕地白浆土的表层土壤,由于受机械耕作影响,土层内混有大量白浆层土壤,土壤紧实,孔隙量减少。白浆层土壤结构呈厚度为2～3mm片状结构,仅有少量水平方向发展的孔隙,无利于土壤排水的纵向孔隙,无作物根系;淀积层土壤呈小核状结构,干缩后沿结构形成裂隙。白浆土的土壤总有效储水孔隙(0.050 0～0.000 2mm)为耕层的16.9%～20.3%。白浆层仅为耕层的67.4%～83.3%,淀积层为耕层的25.6%～54.4%。直径>0.05mm的重力水孔隙耕层在17.8%～24.4%,白浆层和淀积层土壤的重力水孔隙仅1.3%～1.8%和2.4%～6.4%,表明土壤通透性差。直径<0.000 2mm的无效孔隙,淀积层明显高于耕层和白浆层,土壤持水能力强。