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41.
Norman Owen-Smith 《Landscape Ecology》2004,19(7):761-771
Large mammalian herbivores are notorious for their propensity towards population irruptions and crashes, yet many herbivore populations remain relatively stable. I explore how resource heterogeneity within landscapes dampens population instability, using a metaphysiological modelling approach considering patch state distributions. Resource heterogeneity is functionally stabilizing through spreading consumption away from preferred resources before these become critically depleted. Lower-quality resources act as a buffer against starvation during critical periods of the seasonal cycle. Enriching resource quality is destabilizing, even if patch diversity is maintained, because food quantity then becomes the limitation. The potential consequences of landscape fragmentation are explored using the Serengeti ecosystem, characterised by broadscale resource gradients, as a hypothetical example. Further insights provided by the model are illustrated with specific examples concerning the effects of patch scales and waterpoint distribution. A metaphysiological modelling approach enables the basic consequences of landscape heterogeneity to be distinguished from further effects that may arise from specific patch scales and configurations, without the distracting detail of spatially explicit models. 相似文献
42.
Prof. Dr. Peter Lüdders 《Erwerbs-Obstbau》2004,46(4-5):117-122
The carambola (Averrhoa carambola L., family Oxalidaceae) is believed to have originated in Sri Lanka and the Moluccas but it has been cultivated in southeast Asia and Malaysia for many centuries. The evergreen tree is slow-growing, has a rounded crown and reaches 6 to 9 m in height. The 5- to 6-angled fruits (6 to 15 cm long and up to 9 cm wide) have thin, waxy, orange-yellow skin and juicy, yellow flesh. The fruit has a more or less pronounced oxalic acid odor and the flavor ranges from very sour to middle sweetish. The carambola is a tropical and subtropical tree, it can tolerate freezing temperature (?2°C) for short periods and sustain little damage. Trees can be propagated by seed, grafting, budding, and air layering. Tissue culture propagation has met only limited success. Plant spacing varies with growing region and cultural practices. Tree training is practiced intensively in different countries. Harvest timing and postharvest handling are critical to successful marketing of fresh fruit. Carambola is a nonclimateric fruit. Growers carefully harvest fruit by hand at color break — light green fruit with yellow color. The storage conditions should be 5 to 10°C and 85 to 95% relative humidity. Most carambola fruit is eaten fresh, used in salads, desserts, and cooked as star-shaped slices; fruit can also be dried, canned and frozen. The leaves have been eaten as a substitute for sorrel. Commercial production occurs in Taiwan, Malaysia, southern China, the Philippines, Australia, USA (Florida, Hawaii), Brazil, Suriname and Guyana. Production and consumption appears on the rise throughout the world and will most likely increase as selection and breeding for superior tasting cultivars with improved handling characteristics and storage conditions are found. 相似文献
43.
Judith Hübschen Lilo Kling Ulrike Ipach Volker Zinkernagel Nathalie Bosselut Daniel Esmenjaud Derek J.F. Brown Roy Neilson 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(8):779-788
Xiphinema diversicaudatum and X. index are vector nematode species of economic importance in viticulture regions as they can transmit Arabis Mosaic, Grapevine Fanleaf and Strawberry Latent Ringspot viruses to grapevine. Wang et al. (2003) designed species-specific diagnostic primers from ribosomal genes for both these vector species as well as a vector and a non-vector species X. italiae and X. vuittenezi, respectively. Our study aimed to confirm the specificity and determine the sensitivity and reliability of the primers for the two vector species, X. diversicaudatumand X. indexwhen challenged with closely related longidorid species and general nematode communities typical of vineyard soil. With one exception, no PCR product was observed when the primers were tested against six Longidorus, one Paralongidorus and one Xiphinema non-target species. Occasionally (three out of eight replicate PCR reactions) a weak PCR product was noted when primers for X. index were tested with L. elongatus. Furthermore, when challenged with a range of non-target nematode species comprising the nematode community typical of viticulture soil, no PCR product was amplified. An experimental dilution series of extracted DNA rigorously demonstrated that DNA from an equivalent single specimen of the target virus-vector species, X. diversicaudatum and/or X. index, could be detected amongst 1000 equivalent non-targetX. vuittenezi. Also, extracted DNA from an equivalent single target specimen was detected when added to DNA extracted from the overall soil nematode community. The primers were assessed further by using serial mixtures of actual nematodes rather than extracted DNA to simulate field soil. Using this method, a single target nematode could be detected amongst 200 non-target specimens. Given their specificity, sensitivity and reliability, it appears that these diagnostic primers will be of great benefit to phytosanitary/quarantine services related to the viticulture industry. 相似文献
44.
Noha Abou-Madi Peter V Scrivani George V Kollias Sonia M Hernandez-Divers 《Journal of zoo and wildlife medicine》2004,35(2):226-231
Computed tomography (CT) was used to diagnose the cause of lameness in a radiated tortoise (Geochelone radiata) and to determine the extent of shell and skeletal trauma in two snapping turtles (Chelydra serpentina). The radiated tortoise's lameness was ultimately attributed to luxation of the right-shoulder joint that was not detected during plain film radiography. Axial and appendicular fractures were identified in one of the snapping turtles that were not detected during plain film radiography. In each patient, the information obtained during CT provided important diagnostic, therapeutic, or prognostic information. 相似文献
45.
Thomas J Inzana Gretchen E Glindemann Gerald Snider Susan Gardner Lisa Crofton Barbara Byrne Joseph Harper 《Journal of veterinary diagnostic investigation》2004,16(5):374-381
Francisella tularensis type A is the primary cause of tularemia in animals and humans in North America. The majority of research on F. tularensis has been done with the attenuated live vaccine strain (LVS), which is a type B, but very few wild-type F. tularensis strains have been characterized. A gram-negative coccobacillus that was isolated in pure culture from the lungs of a cat that died after being lost for 5 days was received for identification at the Virginia-Maryland Regional College of Veterinary Medicine Teaching hospital. The isolate (strain TI0902) was not identified (or was misidentified) by commercial identification systems; however, it was identified as F. tularensis subspecies tularensis (type A) by sequencing a portion of the 16S ribosomal RNA gene. Furthermore, repetitive extragenic palindromic sequences-polymerase chain reaction amplified a 4-kb DNA fragment from TI0902 that was characteristic of F. tularensis type A but not type B. The electrophoretic profile of the lipopolysaccharide of strain TI0902 was identical to that of the LVS by Western blotting with antiserum to LVS. The protein-enriched outer membrane of strain TI0902 contained 6-8 proteins, which were similar in molecular size to those from the LVS. Electron microscopy of negatively stained and alcian blue-stained LVS and TI0902 cells showed that both strains were coccobacillary in shape and may be encapsulated. However, after mouse challenge, the TI0902 strain was clearly more virulent than the LVS strain. Results of this study indicate that the genotype and phenotype of wild-type F. tularensis type A strain TI0902 is similar, but not identical, to that of the LVS strain. Further studies will help determine whether pathogenesis and host-pathogen interactions are also similar between the 2 strains. 相似文献
46.
Kim M. Plummer Sarah J. Clark Lana M. Ellis Ashwini Loganathan Taha H. Al-Samarrai Erik H.A. Rikkerink Patrick A. Sullivan Matthew D. Templeton Peter C. Farley 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(3):265-274
Peptidases have been implicated in the pathogenicity of fungi that cause disease in plants. Expression of the secreted aspartic peptidase gene (gcsap), of a Glomerella cingulata isolate pathogenic on apples, is induced during appressorium formation. To determine whether the secreted aspartic peptidase (GcSAP) is essential to pathogenicity, gcsap was disrupted using a vector containing a 637 bp fragment of genomic DNA that encodes the sequence spanning the two active site aspartic acid (Asp) residues. To ensure that the truncated gcsap gene products could not have residual peptidase activity the codons for the active site residues Asp112 and Asp297 were both mutated to histidine residues. Both PCR and Southern analysis confirmed disruption of gcsap. Neither gcsap mRNA nor GcSAP activity was detected in the disruption mutant. Pathogenicity tests on fruit from three apple cultivars showed that GcSAP was not required for pathogenicity. The disruption mutant grew on medium containing protein as the sole source of nitrogen because G. cingulata secretes a previously undetected peptidase(s). A serine peptidase that had a pH optimum between pH 7.0 and 8.0 and a K
m of 0.25 mM for the synthetic substrate succinyl-Ala–Ala–Pro–Phe-p-nitroanilide was identified. 相似文献
47.
Marc Lemmens Hermann Buerstmayr Rudolf Krska Rainer Schuhmacher Heinrich Grausgruber Peter Ruckenbauer 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(3):299-308
Fusarium head blight (FHB) is an important disease of wheat, which can result in the contamination of grains with mycotoxins such as deoxynivalenol (DON). Artificial inoculation of flowering ears with conidial suspensions is widely used to study FHB diseases. Our goal was to compare four inoculation treatments in which a conidial suspension was sprayed on flowering ears and to study the effect of the application of moisture during kernel setting and filling with a mist-irrigation system. Ten wheat genotypes were inoculated with a DON-producing Fusarium culmorum strain. Inoculation treatments varied in time of application of the inoculum (morning or evening) and in the method of controlling humidity during inoculation (bagging or mist irrigation). A wet season was simulated with a mist-irrigation system, keeping the crop canopy wet for at least 26 days after flowering. The severity of FHB symptoms (area under disease progress curve (AUDPC)), yield loss and DON contamination in the grains were determined. AUDPC data obtained with the different inoculation treatments were highly correlated (r=0.85–0.95). Mist irrigation after inoculation resulted in a higher mean disease severity, but in a overall lower toxin contamination as compared to the non-irrigated treatments. Genotypic differences in DON accumulation were present: for one wheat line toxin contamination significantly increased when irrigated, while two genotypes accumulated significantly less toxin. The closest relationships (r=0.73–0.89) between the visual symptoms and the DON content were obtained under moderate mean infection pressure. This relation between visual symptoms and the DON content deteriorated at higher infection levels. 相似文献
48.
Prospective long‐term evaluation of parenteral hydroxocobalamin supplementation in juvenile beagles with selective intestinal cobalamin malabsorption (Imerslund‐Gräsbeck syndrome)
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