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41.
Mayada Gwida Maged El-Ashker Falk Melzer Mohamed El-Diasty Mohamed El-Beskawy Heinrich Neubauer 《Irish veterinary journal》2015,69(1):3
Background
Bovine brucellosis remains one of the most prevalent zoonotic infections affecting dairy cattle in developing countries where the applied control programs often fail. We analyzed the epidemiologic pattern of bovine brucellosis in a dairy cattle herd that showed several cases of abortions after regular vaccination with RB51 (B. abortus vaccine). In 2013 thirty dairy cows, from a Holstein-Friesian dairy herd with a population of 600 cattle, aborted five months post vaccination by a regular RB51 vaccine. Blood samples were drawn from milking cows and growing heifers, as well as heifers and cows pregnant up to 6 months. These samples were collected in June 2013 (n?=?257) and May 2014 (n?=?263) and were tested by real time (rt)-PCR as well as serological tests, in particular Rose Bengal Test (RBT), Enzyme-Linked Immunosorbent Assays (ELISA) and Fluorescence Polarization Assay. Tissue specimens were also collected from an aborted fetus and cultured. Isolates were subjected to bacteriological typing tests at the genus and species levels.Results
Five months post vaccination with RB51 vaccine, Brucella (B.) DNA was detected in blood samples of cows by rt-PCR. The serological tests also revealed the spread of Brucella field strains within the herd in 2013. Four Brucella isolates were recovered from specimens collected from the aborted fetus. These isolates were typed as follows: one B. abortus RB51 vaccine strain and three isolates of B. abortus field strain. The seropositive cows with positive rt-PCR might indicate an infection by the Brucella field strain; while the positive rt-PCR results from seronegative animals may either be due to circulating RB51 vaccine DNA in vaccinated animals or to circulating field strain in infected animals before seroconversion.Conclusion
The results herein suggest that PCR can be a good supplementary tool in an outbreak situation, if an assay is available that can differentiate vaccine and field strains with a high analytical sensitivity. We recommend using RBT and ELISA in parallel in outbreak situations, to identify as many infected animals as possible during the initial screenings. This test procedure should be repeated for at least three successive negative tests, with one month interval.42.
43.
Populations at the periphery of a species’ range often show reduced genetic variability within populations and increased genetic
divergence among populations compared to those at the core, but the mechanisms that give rise to this core-periphery pattern
in genetic structure can be multifaceted. Peripheral population characteristics may be a product of historical processes,
such as founder effects or population expansion, or due to the contemporary influence of landscape context on gene flow. We
sampled collared lizards (Crotaphytus collaris) at four locations within the northern Flint Hills of Kansas, which is at the northern periphery of their range, to determine
the genetic variability and extent of genetic divergence among populations for ten microsatellite loci (n = 229). We found low genetic variability (average allelic richness = 3.37 ± 0.23 SE; average heterozygosity = 0.54 ± 0.05
SE) and moderate population divergence (average FST = 0.08 ± 0.01 SE) among our sample sites relative to estimates reported in the literature at the core of the species’ range
in Texas. We also identified differences in dispersal rates among sampling locations. Gene flow within the Flint Hills was
thus greater than for other peripheral populations of collared lizards, such as the Missouri glade system where most of the
mesic grasslands have been converted to forest since the last glacial retreat, which appears to have greatly impeded gene
flow among populations. Our findings signify the importance of considering landscape context when evaluating core-peripheral
trends in genetic diversity and population structure. 相似文献
44.
Ezenduka EV Oboegbulem SI Nwanta JA Onunkwo JI 《Tropical animal health and production》2011,43(3):557-559
The use of antimicrobial agents in poultry production results in their accumulation in the body tissues and products such
as milk and egg. The subsequent accumulation of these drugs and their metabolites in body cells is known as drug residue.
This study was designed to determine the prevalence of antimicrobial residues in eggs from poultry farms and retail outlets
in Enugu State, Nigeria. Eggs from 25 selected commercial farms and ten retail outlets were screened for the prevalence of
antimicrobial residue. Also, structured questionnaires were administered to 25 commercial farms in the state to determine
the management practices and the most widely used antimicrobial drugs in farms and possible association between the management
practices and the occurrence of antimicrobial residues in eggs from these farms. All the 25 farms surveyed use oxytetracycline.
Eggs from nine of the surveyed farms tested positive for antimicrobial residue and three of the ten surveyed farms also tested
positive for antimicrobial residue. No association was observed (p 0.05; Fisher’s exact test) between the occurrence of antibiotic residues in eggs and farm size, feed source and housing systems.
This study was able to demonstrate the presence of antimicrobial residues in eggs destined for human consumption. Drugs like
nitrofurans which has been banned for use in food animals are still very much in use in Enugu State, Nigeria. Antibiotics
given as feed additives may give rise to drug residues in food animals. 相似文献
45.
Booker CW Abutarbush SM Morley PS Guichon PT Wildman BK Jim GK Schunicht OC Pittman TJ Perrett T Ellis JA Appleyard G Haines DM 《The Canadian veterinary journal. La revue veterinaire canadienne》2008,49(3):253-260
The aim of this study was to investigate the effect of bovine viral diarrhea virus (BVDV) infections (unapparent acute infections and persistent infections) on the overall health and performance of feedlot cattle. Calves from 25 pens (7132 calves) were enrolled in the study. Overall and infectious disease mortality rates were significantly higher (P < 0.05) in pens categorized at arrival as positive for type I BVDV and lower in pens that were positive for type II BVDV than in negative pens. Mortality attributed to BVDV infection or enteritis was significantly more common (P < 0.05) in the pens containing persistently infected (PI) calves than in pens not containing PI calves (non-PI pens). There were no statistically detectable (P > or = 0.05) differences in morbidity, overall mortality, average daily gain, or the dry matter intake to gain ratio between PI and non-PI pens. Although type-I BVDV infections in feedlots appear to contribute to higher mortality rates, the presence of PI calves alone does not appear to have a strong impact on pen-level animal health and feedlot performance. 相似文献
46.
A cobalt-mediated decrease in ethylene production reduced the inhibition of nodulation by nitrate in Vigna radiata (mungbean). Nitrate increased the ethylene production in 5-day-old seedlings, while it caused a reduction in the nodulation
status (nodule number and nodule weight) and nodule efficiency (acetylene reduction activity) in mungbean plants. The application
of cobalt chloride inhibited nitrate-affected ethylene production and also decreased the inhibitory effect of nitrate on nodulation.
The effect of cobalt was most marked on nodule number.
Received: 6 August 1999 相似文献
47.
Seong-Mi Jo Yu Ayukawa Sung-Hwan Yun Ken Komatsu Tsutomu Arie 《Journal of General Plant Pathology》2018,84(6):395-398
RNA silencing pathways in filamentous fungi are composed of multiple component proteins and known to be involved in vegetative growth, virulence or sexual reproduction. We found that the tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici (Fol), carries four homologues genes of Qde-2, an argonaute protein gene and one of the main component protein genes in Neurospora crassa. Gene targeting revealed that FoQde-2, one of the Qde-2 homologues in Fol, is involved in virulence to tomato but not in vegetative growth. 相似文献
48.
Rogério M. Borges Alcester Mendes Selene S. C. Nogueira Jérôme Bindelle Sérgio L. G. Nogueira-Filho 《Tropical animal health and production》2017,49(7):1353-1359
A nitrogen (N) balance digestion trial was conducted to determine the protein requirement of collared peccaries (Pecari tajacu). In a 4 × 4 Latin square design, four captive adult male peccaries were fed four isoenergy diets containing four different levels of N (11.7, 16.3, 22.8, and 26.7 g N/kg of dry matter—DM). After 15 days of adaptation, a total collection of feces and urine was carried out for five consecutive days. Regression analyses between N intake and N in feces and urine allowed to calculate the metabolic fecal nitrogen (MFN = 2.3 g N/kg of dry matter intake—DMI) and daily endogenous urinary N (EUN = 185 mg N/kg0.75). Likewise, by regression analyses between consumption of nitrogen and the nitrogen balance (NB = N ingested ? N excreted, mg N/kg0.75), a daily requirement of 514 mg N/kg0.75 was calculated. Therefore, if food intake is unrestricted, collared peccaries require a minimum in their diet of about 5.4% crude protein on DM basis. These values are almost as low as those found for browsing and frugivorous wild ruminants, which reinforce the proposition that peccaries’ digestive physiology is nearer to that of domestic and wild ruminants than domestic pigs. This relatively low protein requirement of collared peccary and its great ability to digest protein reveal the relevance of the forestomach for the species on nitrogen/protein metabolism and allow the use of diets with lower crude protein levels than the commercial ones used for the domestic pig, which reduces feed costs. 相似文献
49.
Leonard Omadang Martin Chamai Emmanuel Othieno Andrew Okwi Francis Olaki Inangolet Francis Ejobi Peter Oba Michael Ocaido 《Tropical animal health and production》2018,50(1):11-17
A cross-sectional study was done from March 2013 to May 2014 to assess knowledge, attitudes, and practices towards cystic echinococcosis (CE) or hydatidosis among selected pastoral and agro-pastoral communities in Uganda. A structured questionnaire was administered to 381 respondents. Multivariate logistic regression analysis was done to find the relationship between knowledge about CE and factors such as age, sex, and level of education across all regions. The odds ratio and confidence interval were used to determine the difference in responses across regions. It was shown that age above 36 years was significantly (p < 0.001) associated with awareness about CE in livestock. Likewise, uneducated (p < 0.0001) and agro-pastoralists (p = 0.01) were significantly less knowledgeable than the educated and pastoralists across all regions. The overall knowledge towards CE in livestock was low 17.8% (95% CI = 14.0–21.6). Dog ownership was high and they never dewormed their freely roaming dogs. Dogs shared water with livestock. In conclusion, knowledge about CE in livestock was low across all regions. Therefore, public health education and formulation of policies towards its control by the relevant stakeholders should be done. Also, the true prevalence of CE in livestock needs to be done so that the magnitude and its public health significance are elucidated. 相似文献
50.
Hayley R. Tumas Brian M. Shamblin Mark Woodrey Nathan P. Nibbelink Richard Chandler Campbell Nairn 《Landscape Ecology》2018,33(9):1585-1601