鸭疫巴氏杆菌的分离和鉴定

从开封地区鸭场发病鸭或病死鸭的脑、心血、肝、脾等脏器中分离到3株革兰氏阴性小杆菌(HP1、HP2、HP3),经形态学检查、生化试验、血清学试验,3株分离菌被鉴定为I型鸭疫巴氏杆菌.药敏试验表明,分离株对蒽诺沙星等抗菌药物都较敏感.     

ORF3 of duck circovirus: a novel protein with apoptotic activity

Duck circovirus (DuCV) is classified in the genus Circovirus of the Circoviridae family. Two major open reading frames (ORFs), encoding the replicase (ORF1/rep) and the capsid protein (ORF2/cap), have been recognized for DuCV. Sequence analysis show that another major conserved ORF (named ORF3) is located in the complementary strand of ORF1/rep of DuCV, and its function remains to be investigated. In this study, the ORF3 of DuCV was expressed in recombinant baculovirus-infected Sf9 cells. By IFA and Western blot analysis, the ORF3 protein was positive for the sera from ducks infected with DuCV. The percentages of apoptotic cells of the Sf9 cells infected with the recombinant baculovirus encoding ORF3 of DuCV were significantly higher than (P<0.05) that of the Sf9 cells infected with wild-type baculovirus at 24, 48 and 72 h postinfection. Based on our knowledge, we deduced that the ORF3 protein of DuCV might play an important role in viral pathogenesis via its apoptotic activity.     

布鲁氏菌病无疫小区建设与成效

近年来,我国将无疫小区管理理念应用到布鲁氏菌病防控,以养殖场生物安全防控作为切入点,整合优化布鲁氏菌病关键技术措施,结合科学有效的监督管理,全面推动布鲁氏菌病防控工作。本文重点从建设主体、生物安全体系、监测要点等方面介绍了布鲁氏菌病无疫小区建设标准,总结了布鲁氏菌病无疫小区建设对于提升完善养殖场生物安全体系,推进养殖企业从传统防控到生物安全管理、风险管理转型升级,促进产业高质量发展等方面的成效,以期对下一步工作推进提供参考。     

我国草原确权承包及信息化管理探讨

草原是我国重要的自然资源,对维护生态系统稳定具有十分重要的意义。草原确权承包工作是草原生态保护和建设的制度保障,是落实中央有关要求的具体举措。从已有对草原确权的报道来看,由于我国草原牧区的特殊情况,草原确权工作在地区间推进速度不一,整体推进不平衡,部分地区确权工作推进相对缓慢。在对草原确权承包工作的背景、现状进行分析的基础上,梳理了推进我国草原确权承包需要的基础工作,包括基础信息收集、承包合同完善、颁发草原权属证书、开展培训、加强信息管理和健全草场纠纷解决机制等,提出了信息管理平台的构建思路,包括主要构成模块、基本功能、工作流程、采用的主要技术等。在此基础上,提出了下一步推进草原“三权分置”的思考和建议,对于推动我国草原确权承包和信息化管理具有重要意义。     

Free choice feeding of whole grains in meat-type pigeons: 1. effect on performance,carcass traits and organ development

1. The effects of 5 different feeding systems on the performance, carcass traits and organ development were studied in pigeon squabs.

2. The 5 treatments were (1) whole grains of maize, pea and wheat plus concentrate feed; (2)whole grains of maize and wheat plus concentrate feed (CWC); (3) whole grains of maize and pea plus concentrate feed; (4)whole grain of maize plus concentrate feed (CC); and (5) compound feed (CF).

3. Feed intake of parent pigeons increased significantly from 0 to 21 d and it was higher in the CF treatment. Body weight of squabs in the CWC treatment was the highest among the 5 treatments in 4 weeks. Body weight losses of parental pigeons during the rearing period were not significantly different among the 5 treatments.

4. Protein intake in CC and CWC treatments was lower than that of the other three treatments. The CWC treatment had the highest daily weight gain and the lowest feed conversion ratio. Treatments were statistically similar in the relative weight of carcass, breast and thigh. CF had the lower relative weight of abdominal fat.

5. Relative weight of gizzard in the CF treatment was significantly lower than that of CWC.

6. It was concluded that the application of free choice feeding of whole grains of maize and wheat plus concentrate feed increased the body weight of 28-d-old pigeon squabs and decreased the feed conversion rate of parent pigeons. This feeding strategy could be commercially interesting in meat-type pigeon production.

     

无公害羊肉的生产技术规范

羊肉蛋白质含量高，脂肪、胆固醇及饱和脂肪酸含量低．富含矿物质和维生素，是肉类中的佳品．然而环境污染、饲草料农残、以及不合理的使用或滥用兽药和药物添加剂．导致许多有毒有害物质直接或通过食物链进入羊体内．同时羊的许多疾病可通过产品传播给人．从而影响其食用的安全性。[第一段]     

加强兽药监管,生产安全畜产品

兽药是一种特殊商品,在畜业生产中被广泛使用,如使用不当不仅影响动物疫(疾)病防治效果,而且通过食物链间接影响人类的健康和生命。     

引进三眠蚕诱导技术生产超细纤度生丝的试验及其开发意见

安徽金寨县引进三眠蚕诱导技术生产超细纤度生丝 ,并就开发生产超细纤度生丝提出建议意见     

灰色关联分析在草坪草引选评价上的应用

根据灰色理论和应用数列间的相似程度来判断两个系统或系统中的两个因素之间关联程度的原理，在对引进的３个草坪草种（１４个品种）多种性状观测及分析的基础上，计算求得各性状的关联系数ε１（ｋ）及关联度γｉ，综合评价各引选品种，由定性评价转为定量评价。结果表明，在贺兰山东麓地区，表现较好的草坪草种有：草地早熟禾类的瓦巴斯、菲尔京、纳苏、爱肯尼４个品种：紫羊茅中的巴可紫羊茅、高羊茅中的梯坡，巴法７８５１两个品     

土壤硒富集空间分布特征及影响因素研究

以揭阳市土壤为对象,系统采集了表层土壤样(0～20 cm)1 330个和深层土壤样(150～200 cm)331个,并利用相关性分析、回归分析、方差分析及GIS空间分析技术等方法对土壤硒的含量分布、富集特征及影响因素进行了系统的分析。结果表明,揭阳市表层土壤Se含量处于0.02～2.01 mg/kg之间,几何平均值为0.48 mg/kg,是中国土壤Se平均含量的1.66倍。揭阳市土壤总体呈足硒及富硒特征,不存在硒过剩,极少区域土壤呈硒缺乏特征,表层与深层土壤表现基本一致,富硒土壤主要分布于普宁市、惠来县及北部边缘。表层土壤中Se富集面积达到52.03%,但在空间上分布零散,这可能与母质、土壤类型等因素有关。强富集区域集中分布于花岗岩与粉砂岩为母质的土壤区,而大部分由第四纪冲积物形成的土壤无富集。方差分析表明:不同母质、土壤类型及土地利用方式对土壤Se的含量及富集水平均造成不同程度的影响,其中影响揭阳市表层土壤Se含量的主要因素为成土母质。除此之外,土壤理化性质及海拔也是影响揭阳市表层土壤Se富集的重要因素。回归分析表明表层土壤Se与pH值呈极显著的负相关,并且分别与有机碳、Fe_2O_3及Al_2O_3呈极显著线性正相关。