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Na Wang Kang Yang Hai‐Tao Guo Jing‐Ran Wang Huan‐Huan Sun Shun‐Wei Wang Meng Sun Liang‐Zheng Sun Shun‐Li Yue Jia‐Bo Zhou 《Reproduction in domestic animals》2019,54(8):1069-1077
Spermatozoa are highly specialized cells, and energy metabolism plays an important role in modulating sperm viability and function. Rosiglitazone is an antidiabetic drug in the thiazolidinedione class that regulates metabolic flexibility and glucose uptake in various cell types, but its effects on boar sperm metabolism are unknown. In this study, we investigated the potential effect of rosiglitazone against time‐dependent deterioration of boar spermatozoa during liquid preservation at 17°C. Freshly ejaculated semen was diluted with Beltsville Thawing Solution (BTS) containing different concentrations of rosiglitazone, and the motility, membrane and acrosome integrity of sperm were detected. Besides, we measured glucose uptake capacity, l ‐lactate production level, mitochondrial membrane potential, adenosine triphosphate (ATP) content and mitochondrial reactive oxygen species (mROS) production of sperm after boar semen had been incubated with or without rosiglitazone, iodoacetate (glycolysis inhibitor) and rotenone (electron transport chain inhibitor) for 5 days. The addition of rosiglitazone significantly enhanced sperm quality and had a strong protective effect on the sperm membrane and acrosome integrity during storage. BTS containing 50 μM rosiglitazone maintained the total motility of liquid‐preserved sperm above 60% for 7 days. Rosiglitazone improved sperm quality by regulating energy metabolism manner of preserved sperm, protected the sperm mitochondrial membrane potential, enhanced sperm ATP production and in the meanwhile reduced mROS through enhancing glycolysis but not oxidative phosphorylation. The data suggested the practical feasibility of using rosiglitazone for improving boar spermatozoa quality during semen preservation. 相似文献
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Expression of Mitochondria‐Associated Genes (PPARGC1A,NRF‐1, BCL‐2 and BAX) in Follicular Development and Atresia of Goat Ovaries 下载免费PDF全文
G Zhang Y Wan Y Zhang S Lan R Jia Z Wang Y Fan F Wang 《Reproduction in domestic animals》2015,50(3):465-473
Most follicles undergo atresia during the developmental process. Follicular atresia is predominantly regulated by apoptosis of granulosa cells, but the mechanism underlying apoptosis via the mitochondria‐dependent apoptotic pathway is unclear. We aimed to investigate whether the mitochondria‐associated genes peroxisome proliferator‐activated receptor‐gamma, coactivator1‐alpha (PPARGC1A), nuclear respiratory factor‐1 (NRF‐1), B‐cell CLL/lymphoma 2 (BCL‐2) and BCL2‐associated X protein (BAX) played a role in follicular atresia through this pathway. The four mitochondria‐associated proteins (PGC‐1α, which are encoded by the PPARGC1A gene, NRF‐1, BCL‐2 and BAX) mainly expressed in granulosa cells. The mRNA and protein levels of PPARGC1A/PGC‐1α and NRF‐1 in granulosa cells increased with the follicular development. These results showed that these genes may play a role in the regulation of the follicular development. In addition, compared with healthy follicles, the granulosa cell in atretic follicles had a reduced expression of NRF‐1, increased BAX expression and increased ratio of BAX to BCL‐2 expression. These results suggested that changes of the mitochondria‐associated gene expression patterns in granulosa cells may lead to follicular atresia during goat follicle development. 相似文献
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为评估罗非鱼湖病毒(tilapia lake virus,TiLV)跨境传入风险,按照OIE传入风险评估框架,从危害识别、风险评估及风险管理3个方面,开展了TiLV跨境传入风险分析,并提出了相应的风险控制措施。分析认为:活的敏感鱼类(包括亲鱼、鱼卵和鱼苗),用做鲜活饵料的鱼类(野杂鱼)及冰冻和冰鲜的敏感鱼类(包括去除内脏的鱼肉)TiLV传入风险较高,而敏感鱼加工类产品、非敏感鱼类(包括活的和其他形式的产品)及运输活鱼的水、包装、运输工具和用具等传入风险较低。建议不从疫区进口高风险产品,对于低风险产品可以自由贸易,而对运输活鱼的水、包装、运输工具和用具等要进行强制常规消毒。 相似文献
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采用传染性支气管炎弱毒苗对1日龄雏鸡分别进行点眼和滴鼻免疫.取气管和哈德氏腺做石蜡切片,以葡萄球菌A蛋白-辣根过氧化酶(SPA-HRP)免疫组化染色,对气管和哈德氏腺中SIgA细胞的分布、数量变化进行了观察.结果表明:SIgA阳性细胞分布在哈德氏腺的间质中;气管中的SIgA细胞主要分布在黏膜上皮细胞之间和固有层中,局部黏膜上皮形成的隐窝内也分布有SIgA细胞.数量统计分析表明:免疫组的哈德氏腺和气管中SIgA细胞数量显著高于对照组;点眼免疫的SIgA细胞数量比滴鼻免疫的高;加强免疫则使SIgA细胞数量进一步增多. 相似文献
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我国农业灾害损失相当严重,农业保险是有效分散农业风险促进农业和农村经济发展的重要手段,针对我国实际国情,选择合适的农业保险发展模式十分重要。本文在分析了国内外各种农业保险发展模式的基础上,提出了我国农业保险发展模式。并对其进行了深入分析。 相似文献