平菇2潮后菌棒畦式覆土法和菌墙法效果比较

袋栽平菇2潮后菌棒失水干缩,能否补水成为限制出菇的主要因子。脱袋覆土成了补水兼顾补养的优良措施,方法主要有畦式覆土法和泥菌墙法,为了弄清两者优劣以便更好地指导平菇生产,特进行比较试验。结果表明,畦式覆土法更易于补水、补养,不仅管理方便,而且出菇潮次多、转潮快、出菇商品性高、生物转化率大。相比之下,泥菌墙法在补水、补养上不易使上下层均匀,在上层已显缺水时,下层还在水渍状态,且平菇易污染泥水而导致品质下降。另外,泥茵墙法中平菇转潮慢,生物转化率小,容易积温。平菇2潮后菌棒畦式覆土栽培法是获得高产的优选措施。     

Inhibitory effect of anti-miR-196a on invasion and migration of human pancreatic cancer PANC-1 cells

AIM:To investigate the expression of miR-196a in different pancreatic cancer cell lines and to observe the effect of anti-miR-196a on the biological behaviors of human pancreatic cancer PANC-1 cells. METHODS:The expression of miR-196a in the pancreatic cancer cells was examined by real-time quantitative PCR. Anti-miR-196a was chemically synthesized and transfected into PANC-1 cells by Lipofectamine 2000. The cell proliferation was measured by CCK-8 assay. The apoptosis was determined by flow cytometry. Matrigel invasion assay was performed to examine the migration and invasion of the tumor cells. The wild-type and mutant-type NF-κB inhibitor α(NFKBIA) 3'UTR luciferase reporter vectors were constructed. The relative activity of Renilla luciferase was detected to confirm the binding site of miR-196a on NFKBIA mRNA. RESULTS:The expression of miR-196a in human pancreatic cancer cell lines was significantly higher than that in human pancreatic ductal epithelial H6c7 cells. The expression of anti-miR-196a in miR-196a group was down-regulated. After transfected with miR-196a, no change of cell proliferation and apoptosis was observed, but the abilities of invasion and migration were reduced(P<0.01). Compared with negative control, wild-type NFKBIA3'UTR or mutant-type NFKBIA 3'UTR, cotransfection of anti-miR-196a and wild-type NFKBIA 3'UTR significantly increased the relative activity of Renilla luciferase. CONCLUSION:miR-196a is one of the oncomiRs and may be a target microRNA of human pancreatic cancer for gene therapy.     

卡姆罗莎草莓离体培养快繁技术

卡姆罗莎组培繁殖最佳诱导丛生芽配方应为MS+6-BA 1.0 mg/L,其次为MS+ 6-BA 1.0 mg/L+N从0.1 mg/L.选取最佳增殖培养基的配方为:MS+6-BA 0.5 mg/L,继代培养基的配方为MS+6-BA 0.25 mg/L.生根培养基不需加任何激素,只需将生根苗接到1/2MS 培养基上即可.当试管苗长到4 cm左右,根系长2～3 cm时,把培养瓶移出培养室.     

园林植物废弃物堆肥的理化性状及参数研究

以园林植物废弃物为原料,采用高温好氧堆肥技术,运用正交实验研究了含水率、C/N、菌种和翻堆对堆肥的影响。结果表明:基于种子发芽指数的适宜参数是含水率51.2%、C/N30.9、菌种用量10 g/kg、翻堆时间5 d,4个因素对堆肥影响的大小顺序是:含水率、C/N、翻堆时间、菌种用量。堆肥过程堆料体积缩小,pH和EC值升高,堆肥成品具有一定的肥力。     

诱导Badila提早开花的主要气象因子初探

通过连续两年人工诱导结果表明,提早诱导时间、暗期气温高低、光照期间破坏性高温天数是诱导Badfla提早开花的主要原因。     

Effects of iPSC-MSCs on mitochondria of PC12 cells injured by CoCl2

AIM: To investigate the effects of induced pluripotent stem cells-derived mesenchymal stem cells (iPSC-MSCs) on cobalt chloride (CoCl₂)-induced injuries of PC12 cells and its possible mechanism. METHODS: PC12 cells were exposed to CoCl₂ to set up a chemical-induced cellular injury model and were cocultured with iPSC-MSCs. The cell viability was tested by CCK-8 assay. The apoptosis was measured by flow cytometry using Annexin V/PI staining. The mitochondrial membrane potential (MMP) was analyzed by flow cytometry using JC-1 staining. Immunofluorescence was employed to observe mitochondrial transfer from iPSC-MSCs to PC12 cells. RESULTS: Apoptosis of PC12 cells was increased and MMP of PC12 cells was decreased after exposed to CoCl₂ at concentration of 400 μmol/L for 24 h. Coculture of PC12 cells with iPSC-MSCs reduced the apoptosis and recovered the MMP of the PC12 cells. Tunneling nanotubes were formed between iPSC-MSCs and PC12 cells, through which the iPSC-MSCs transferred the mitochondria to the PC12 cells. CONCLUSION: iPSC-MSCs protect PC12 cells from CoCl₂-induced injuries, which may be associated with the mitochondrial transfer from iPSC-MSCs to PC12 cells.     

保护地菌菜套作菌料用量对土壤呼吸速率影响的研究

采用日光温室番茄架下套种双孢菇小区栽培试验的方法,研究了4种菌料用量(0、255、07、5 t/hm2)与土壤呼吸速率、土壤温度之间的关系。结果表明:菌菜套作模式能明显提高5 cm地温和土壤呼吸速率,投施菌料的各处理5 cm深处土壤温度,土壤呼吸速率与未施菌料的对照之间差异均达到了1%显著水平。当菌料投施量在48.6~56.4 t/hm2范围内时,具有用量少,地温高,增产效果好等优点,应在日光温室内番茄架下套种双孢菇栽培模式中优先选用。     

αvβ6-ERK pathway participates in norcantharidin-induced apoptosis of HT-29 colon cancer cells

AIM: To investigate the pharmacological mechanism of norcantharidin (NCTD)-induced apoptosis of HT-29 colon cancer cells. METHODS: Hoechst 33258 staining was used to analyze the apoptosis of HT-29 cells treated with NCTD. The effects of NCTD on the expression of integrin in HT-29 cells were determined by flow cytometry. The effects of several functional blocking antibodies on HT-29 cells were detected by MTT method. The expression and the phosphorylation of mitogen activated protein kinases (MAPKs) in HT-29 cells were measured by Western blotting. Co-immunoprecipitation assay was used to detect the activity of αvβ6-extracellular signal-regulated kinase (ERK) direct linkage in HT-29 cells.RESULTS: NCTD induced the apoptosis of HT-29 colon cancer cells. The expression of integrin αvβ6 in HT-29 cells treated with NCTD was reduced, but the expression of αvβ3 and αvβ5 was not changed. A function-blocking antibody to αvβ6,10D5,strengthened the growth inhibitory effect of NCTD on HT-29 cells ,but LM609 (a function-blocking antibody to αvβ3) and P1F6 (a function-blocking antibody to αvβ5) did not. The level of phosphorylated ERK (p-ERK) was decreased substantially after treated with NCTD in a dose-and time-dependent manner. NCTD also affected the association of αvβ6 and ERK. CONCLUSION: NCTD decreases the expression of integrin αvβ6 and interferes with the phosphorylation of ERK. As a result, the formation of αvβ6-ERK direct linkage is affected and the signal transduction mediated by αvβ6 is disturbed. The mechanism of NCTD-induced HT-29 cell apoptosis is involved in the αvβ6-ERK signaling pathway.     

城市园林栽植土质量标准的对比分析

通过对城市园林栽植土质量标准的对比分析。结果表明:5个标准在园林栽植土的分类方面差异较大,相同分类只有花坛土和草坪土;对栽植土的一般质量要求总体相近,但是在质量评定、指标选择和指标值设定方面存在较大差异,这与城市土壤研究现状、地区土壤特性和园林建设情况等密切相关。为进一步提高我国城市园林栽植土的管理,提供理论依据。     

2种常见忍冬科植物的抗旱性综合评价

以生长在上海同一绿地中的忍冬科常见园林植物锦带花和大花六道木为试验材料,采用自然干旱胁迫方法,对2种植物的8个抗旱指标进行测定,并用模糊数学隶属函数值法对2种植物的抗旱性进行综合评价.结果表明:大花六道木的抗旱性显著强于锦带花的抗旱性;同时,植物抗旱性是一个综合指标,不应由单一指标决定