小麦区试品系DUS测试的分子标记

为了确定测试小麦(Triticum aestivum L.)区试品系特异性、一致性、稳定性(DUS)的分子标记,采用156个来自我国不同麦区的品种对SSR、EST-SSR和AFLP-SCAR标记的1334对引物进行筛选,根据在染色体上分布均匀、多态性信息指数较高、带型清晰、不同等位变异的带型易于区分及PCR产物稳定的原则,筛选出105对小麦品种DUS测试的分子标记引物,包括63对SSR引物、21对EST-SSR引物和21对AFLP-SCAR引物,可以检测122个位点的754个等位变异,平均每条染色体上被检测位点5.8个,平均每个位点包含7.2个等位变异。根据DUS测试的需求、引物的染色体分布、PIC值大小和带型特点,将105对引物分为21对核心引物、29对一级备用引物和55对二级备用引物。核心引物分辨力较高,可以完成约80%品系的特异性检测,约95%品系的种子纯度检测和约60%品系的一致性、稳定性检测;备用引物用于确定品系DNA位点纯合率和相似品种(品系)之间的遗传相似系数,以判断DNA指纹相同或相似的品种(品系)之间的相似性和特异性,评价核心标记中具有非纯位点的品系的DNA位点纯合度,同时完成核心引物未能完成的少数品系的种子纯度检测。通过在2006-2007、2007-2008、2008-2009年度对464个冬小麦区试品系DUS测试中的应用,证明105对引物具有很好的代表性和实用性,可以完成90%以上参试品系的DUS检测。     

Theoretical Contour Line of Reinforcement Bar of Non uniform Corrosion

Most of the previous studies on corrosion of the steel bar in reinforced concrete structures is based on the assumption that the bar is uniformly corroded. The actual measurement in projects proved that most of the reinforcement bar is in non uniform corrosion state. Based on electrochemical principles of metal corrosion and combined with the real corrosion course of the bar in concrete structures, a theoretical model of the contour line of reinforcement bar due to non uniform corrosion is established, through deducing the speed of corrosion and theoretical corrosion penetration and volume changes due to corrosion of reinforcement in general air at different position and different time. At the same time, the model is verified with the results from relative references and measured data.     

Investigations of the particle motion characteristics in the gas solid two phase wake of leeward side circular cylinder

This paper investigates the characteristics of the particle motion in circular cylinder wake flow of leeward side by simulating the micro particle flow around circular cylinder using Euler two fluid model combined with Reynolds stress model. It compares the particle velocity and concentration of different particle size in the leeward area. The results show that the vortex is formed after the gas flowed passing the cylinder, and the concentration and velocity of particles are affected by the turbulent intensity in the leeward side. Gas entrainment vortex and particles own inertia decide the flow form of fine particle around the cylinder. Both effects impact the particle concentration distribution of different size particles in the leeward area. With the increase of particle size, the particle concentration increases at the beginning and then decreases in the leeward area.     

Nonlinear numerical simulation of steel reinforced concrete truss joints

This paper analyzes the steel reinforced concrete large span transfer truss joints using the finite element analysis software ANSYS, and get the joints deformation and the mechanics increase, such as deformation properties, stress distribution. The monitoring results during construction phase show that nonlinear numerical simulation analysis, which is in good agreement with the test results, is an effective method for the mechanical property analysis on this kind of structural components.     

Embedded terminal receiving capability matching in multimedia file transmission

A novel transport protocol for large scaled multimedia file EMFTP (Embedded Multimedia File Transfer Protocol) is proposed to match the receiving capability of embedded terminal with sufficient network bandwidth. EMFTP is an application level protocol using UDP, and adopts a method of uniform retransmission as its error recovery strategy and it also introduces an improved DAIMD (AIMD with decreasing increases) rate control algorithm based on bandwidth probe. It can improve the data transfer rate with a low resource taken of embedded client. As the experimental result shows, compared with UDT and FTP protocol implemented on embedded terminal, EMFTP data transfer rate is increased by 3% and 17% respectively, the embedded client CPU occupancy ratios are reduced by 50.3% and 42.3% separately, and memory usage reduced by 15% and 4%.     

五个鲜食玉米新品种的DNA指纹图谱研究与应用

利用SSR标记技术，对5个鲜食玉米新品种及其亲本的DNA指纹图谱进行了研究。从88对SSR引物中，筛选出37对在鲜食玉米中表现多态性丰富、带型清晰明显且稳定的引物，分析了鲜食玉米自交系的遗传多样性，建立了各品种的特征DNA指纹图谱及数字指纹，可以有效用于种子质量检测。     

苦参种子发芽特性研究

对苦参种子硬实的最佳破除方法、最适发芽的温度、光照条件及盐浓度对种子萌发的影响进行了研究.结果表明:98%浓硫酸处理、烫种和砂纸磋磨处理均可提高苦参种子的发芽率,其中以98%浓硫酸处理40min为最佳处理方法;25℃为苦参种子发芽的最适温度,光照条件非苦参种子萌发的必备条件;适量的NaCl(＜5 g/L)有利于提高苦参种子的发芽势和发芽率,高浓度的NaCl(＞7 g/L)则明显抑制种子萌发.     

大豆染色体代换系CB4F2回交群体的构建及聚类分析

2000-2008年,以山西省骨干亲本晋豆23号(Glycine max Merr,cv.Jinbean 23)为母本、农家品种灰布支(Glycine max Merr,cv.ZDD2315)为父本配置杂交组合,通过多代不明确回交和自交构建446个株系的大豆染色体代换系群体,并通过聚类分析方法对群体进行聚类,将其初步分为晋豆23号等位系核心群Ⅰ(161份),次核心群Ⅱ(172份),灰布支近血缘品系群Ⅲ(68份),超亲品系群IV(45份),这将为大豆遗传图谱研究、基因功能组研究和功能基因克隆提供理想丰富的遗传群体和材料平台.     

正交试验法解决卷烟小盒包装凹陷

采用正交试验设计方法,对影响泰山(儒风)牌卷烟小盒包装凹陷问题的透明纸烙铁温度、小盒商标纸尺寸和小盒商标纸挺度3因素进行了试验。结果表明,3因素中透明纸烙铁温度是造成小盒凹陷的主要因素,小盒商标纸尺寸和小盒商标纸挺度为次要因素。     

山东省苹果冷藏业发展现状与对策

概述了山东省苹果冷藏业经营与苹果产区的发展状况．分析了苹果冷藏产业经营中存在的问题,提出了应采取的对策。