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71.
Krzysztof Dziedzic Danuta Górecka Artur Szwengiel Hanna Sulewska Ivan Kreft Elżbieta Gujska Jarosław Walkowiak 《Plant foods for human nutrition (Dordrecht, Netherlands)》2018,73(1):82-88
In this report, we presented the profile of polyphenolic substances in flowers, leaves, stalk and roots of Fagopyrum tataricum estimated by using RP–UHPLC–ESI-MS equipment (reversed-phase ultra-high-performance liquid chromatography electrospray ionisation mass spectrometry). The neutral detergent fibre, acid detergent fibre, acid detergent lignin, cellulose and hemicellulose were also determined. Flowers, leaves, stalk and roots showed varying levels of dietary fibre and polyphenols. The highest content of neutral and acid detergent fibre were found in the roots (63.92 and 45.45% d.m., respectively) while the most rich in phenolic compounds were flowers (4.8 mg/1 g d.m.). Root and stalk contained the highest level of cellulose, 38.70 and 25.57% d.m., respectively. Among the investigated polyphenolic substances such as: 2,6-dihydroxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,5-dihydroxybenzoic acid, 4-hydrobenzoic acid, caffeic acid, catechin, chlorogenic acid, fagopyrin, ferulic acid, myricetin, gallic acid, isovanilic acid, isovitexin, kaempferol, luteolin, p-coumaric acid, procyanidin B2, quercetin, quercetin 3-D galactoside, rutin, syringic acid and vitexin, we observed that the contents of rutin and chlorogenic acid were the highest. We found some correlation between dietary fibre fractions and individual phenolic substances. The levels of acid detergent fibre (ADF), cellulose and hemicellulose were negatively correlated with isovitexin, kaempferol, vitexin, fagopyrin, caffeic acid and procyanidin B2 content. In this investigation, two solvents (water and methanol) were estimated regarding their extraction efficiency of phenolic compounds. Taking these results into consideration, we recommend using methanol as the extractor to isolate chlorogenic acid, fagopyrin, kaempferol, procyanidin B2, quercetin, quercetin 3-D-galactoside, rutin, vitexin, and water for other investigated polyphenolic substances obtained from Fagopyrum tataricum. 相似文献
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Berta Lasa Ivan Jauregui Iker Aranjuelo Sandra Sakalauskiene Pedro M. Aparicio-Tejo 《Journal of plant nutrition》2016,39(1):87-98
Poplar cuttings (Populus x euroamericana) were planted in pots and grown in a greenhouse for four months with drip irrigation. Two nitrogen (N) doses of 60 and 120 kg nitrate (N-NO3?) ha?1 (labeling with 15N stable isotopes) were applied at one (time I), two (time II) and three months after of the plantation (time III). The aim of this study was to determine the N-fertilizer application method that resulted in optimal N-uptake by a short-rotation of poplar crop during first year of growth. Our data revealed that NUE (N use efficiency) was largest in the last part of the experiment. The larger development of those plants at this stage enabled the improved absorption and assimilation of N. Furthermore, 15N also revealed that supply of N at three months post-planting generated more biomass and increased the N reserves in the stem; a key factor for ensuring the optimum regrowth during the second year. 相似文献
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Tomas Navratil Jan Rohovec Aria Amirbahman Stephen A. Norton Ivan J. Fernandez 《Water, air, and soil pollution》2009,201(1-4):87-98
We experimentally determined the adsorption characteristics of natural, freshly precipitated Al(OH)3 for SO4 and PO4. The fresh Al precipitate occurred in stream sediment of Jachymov Stream (Czech Republic). The Al-rich sediment strongly adsorbed added PO4 prior to acidification experiment; this sorbed PO4 was released only after substantial dissolution of the sediment, at pH?<?3.67. Sorption of P by Al(OH)3 appears to be an important control on dissolved PO4 concentration in surface waters, unless there is a large excess of PO4. Acidification of the sediment-solution system caused protonation of the sediment surface, thereby increasing the adsorption capacity for SO4. Maximum SO4 adsorption occurred at pH 4.2, below which dissolution of the sediment offset the increasing anion adsorption capacity, and formation of AISO4 + inhibited the increasing SO4 adsorption capacity. This research demonstrates that there are important pH thresholds for anion adsorption in freshwaters below which dissolution of the Al(OH)3 substrate reduces total capacity for anion adsorption. In freshwaters, with sufficient concentrations of suspended Al(OH)3, or in Al(OH)3-rich sediment, PO4 mobility will be severely restricted. Suspended Al(OH)3 in acidified surface waters cannot strongly influence SO4 concentrations because of the considerably higher total SO4 concentrations compared to the available surface area. 相似文献
76.
Ternjej Ivančica Stanković Igor Mihaljević Zlatko Furač Lidija Želježić Davor Kopjar Nevenka 《Water, air, and soil pollution》2009,204(1-4):299-314
Water, Air, & Soil Pollution - In this pilot study, we evaluated the usefulness of the alkaline comet assay as a biomonitoring tool at the level of plankton communities affected by varying... 相似文献
77.
Eva van Gorsel Nicolas Delpierre Ray Leuning Andy Black J. William Munger Steven Wofsy Marc Aubinet Christian Feigenwinter Jason Beringer Damien Bonal Baozhang Chen Jiquan Chen Robert Clement Kenneth J. Davis Ankur R. Desai Danilo Dragoni Sophia Etzold Thomas Grünwald Lianhong Gu Bernhard Heinesch Lucy R. Hutyra Wilma W.P. Jans Werner Kutsch B.E. Law Monique Y. Leclerc Ivan Mammarella Leonardo Montagnani Asko Noormets Corinna Rebmann Sonia Wharton 《Agricultural and Forest Meteorology》2009,149(11):1919
Micrometeorological measurements of nighttime ecosystem respiration can be systematically biased when stable atmospheric conditions lead to drainage flows associated with decoupling of air flow above and within plant canopies. The associated horizontal and vertical advective fluxes cannot be measured using instrumentation on the single towers typically used at micrometeorological sites. A common approach to minimize bias is to use a threshold in friction velocity, u*, to exclude periods when advection is assumed to be important, but this is problematic in situations when in-canopy flows are decoupled from the flow above. Using data from 25 flux stations in a wide variety of forest ecosystems globally, we examine the generality of a novel approach to estimating nocturnal respiration developed by van Gorsel et al. (van Gorsel, E., Leuning, R., Cleugh, H.A., Keith, H., Suni, T., 2007. Nocturnal carbon efflux: reconciliation of eddy covariance and chamber measurements using an alternative to the u*-threshold filtering technique. Tellus 59B, 397–403, Tellus, 59B, 307-403). The approach is based on the assumption that advection is small relative to the vertical turbulent flux (FC) and change in storage (FS) of CO2 in the few hours after sundown. The sum of FC and FS reach a maximum during this period which is used to derive a temperature response function for ecosystem respiration. Measured hourly soil temperatures are then used with this function to estimate respiration RRmax. The new approach yielded excellent agreement with (1) independent measurements using respiration chambers, (2) with estimates using ecosystem light-response curves of Fc + Fs extrapolated to zero light, RLRC, and (3) with a detailed process-based forest ecosystem model, Rcast. At most sites respiration rates estimated using the u*-filter, Rust, were smaller than RRmax and RLRC. Agreement of our approach with independent measurements indicates that RRmax provides an excellent estimate of nighttime ecosystem respiration. 相似文献
78.
A procedure for the production of conjugates of soybean peroxidase (SbP) oxidized by sodium periodate and anti-mouse IgG antibody (Ab) was optimized. A sandwich chemiluminescent enzyme-linked immunosorbent assay (ELISA) for determination of mouse IgG using SbP and specific Ab was developed, and SbP-catalyzed oxidation of luminol was carried out in the absence of any enhancer. Comparison of conjugates produced by labeling Ab by soybean and horseradish peroxidases in the chemiluminescent ELISA showed that in the case of SbP a rate of emission decay formed through luminol oxidation was significantly lower. Application of the soya enzyme allowed the development of the immunoassay with improved sensitivity and a wider linear range. 相似文献
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80.