鸡卡氏杆菌的分子鉴定及其16S rRNA基因序列分析(英文)

Two strains of Gallibacterium, isolated from one laying hen flock in Zhengzhou City of Henan Province, were identified by the morphological observation, genus-specific PCR, and analysis of 16S rRNA gene, which was used to generate the phylogenetic tree, with the 21 members of the 12 genera belonging to Pasteurellaceae to analyze the homology. Two strains were named Yu-ZZ-HL-I-SLG and Yu-ZZ-HL-II-GZ. The comparative result of the 16S rDNA sequence shows that the 2 isolated strains are identical in sequence; the highest identity (99.9%) was observed between the isolated strain and one of the strains of Gallibacterium anatis (AF228002), the homologies between the isolated strain and 3 strains of gallibacterium accessed in NCBI (AF228016, Gallibacterium genomosp.1, AF228017, Gallibacterium genomosp.2, AF228018, Gallibacterium genomosp.1) were above 97.1%, higher than that of the isolated strain and the other strains of the other 11 genera which were between 90.7%-93.2%. It can be seen from the phylogenetic tree that the 2 isolated strains and the other 4 strain of gallibacterium fell into the same branch, furthermore the 2 isolated strains and the strain of Gallibacterium anatis locate in an internal branch, indicating that the 2 isolated strains belong to Gallibacterium anatis.     

Progress in Research of Chinese Wetland Parks

There has been a rapid development in the construction of wetland parks in China in recent years.This paper discussed the progress in the research of Chinese wetland parks that covered the aspects of wetland resources,landscape and environment.The function of wetland parks,the evaluation of landscape and the exploiting of resources;the landscape concept and landscape planning and design;the plant environment,the water environment and the recreation environment were all reviewed.The research of Chinese wetland parks started later but developed rapidly,having remarkable achievements in practice and functional studies,and there are still some shortcomings in the researches to be improved.At last,the key points which should be researched urgently in the future were discussed.     

The Application of Quantitative Methods to the Landscape Architecture Science of China

Influenced by traditional methods in the landscape architecture,the studies in landscape architecture science of China are fundamentally subjective and qualitative,and aim at aesthetic elements.The quantitative methods are commonly used in the physicism domain,and there is a great potential for the application of quantitative methods to landscape architecture science.The paper summarized the progress in the application of quantitative methods to landscape architecture science in China,in which the evaluation of eco-effect,sightseeing effect,result of disposing,economic benefit,engineering quality,greenbelts landscape,landscape patterns and usage of greenbelts,etc.were involved.It proves that quantitative methods have aroused general concern and have been well applied in certain fields and there are still some shortcomings in the researches to be improved.Finally,the important directions of Chinese landscape architecture science are proposed.     

不同化学药剂对黄花乌头种子萌发的影响

通过用三种不同药剂KMnO4（0．4％、0．8％、1．2％）、6-BA（10μg．g^-1、50μg．g^-1、100μg．g^-1）、CA3（100mg／mL、200mg／mL、300mg／mL）浸种12h后,调查黄花乌头种子萌发启动日、高峰日、发芽率及发芽势。结果是GA3200mg／mL浸种,黄花乌头种子发芽卒及发芽势最高,达91．24％和72．52％,效果最佳。     

Studies on the Relationship between Occurrence Degree of Cotton Bollworm and Rainfall

[Objective] The aim of the study is to investigate the factors causing the outbreak of cotton bollworm and to provide effective measures for controlling cotton bollworm. [Method] Based on the analysis of the data about insect and weather situation in Luyi County in 32 years,the meteorological prediction model was established for monitoring the quarterly or monthly occurrence trend of cotton bollworm. [Result] The cotton bollworm occurred slightly in the years with rainfalls of 3 months over 500 nm and severely in the years with rainfalls of 3 months less than 400 nm. The results of correlation analysis show that annual occurrence degrees of cotton bollworm and occurrence degrees of 4th generation of cotton bollworm are extremely negatively correlated with rainfall during June-August; the occurrence degrees of 3rd and 4th generations of cotton bollworm are also extremely negatively correlated with rainfall in July. [Conclusion] The occurrence of cotton bollworm in field is heavily influenced by rainfall in at its occurrence stage,moreover,the rainfall during June-August is the decisive factors influencing the occurrence of cotton bollworm.     

冷休克诱导黄河鲇(Silurus asotus)二倍体雌核发育(英文)

[Objective] The cold shock method was used to induce diploid gynogenesis of Silurus astus. [Mthod] The Silurus astus sperms were irradiated by ultraviolet,then conducted for fertilization. The different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus astus.[Result] Under such inducing condition that ultraviolet irradiated 15 min,cold shock started 5 min after fertilization,and cold shock duartion was 40 min,the survival rate of diploid Silurus astus reached the highest(8.5%). [Conclusion] The experiment laid foundation for culturing new excellent Silurus astus variety and accumulated original files for further study of gynogenesis development mechanism.     

New Varieties of Populus tomentosa Carr. and Breeding Techniques

The main morphological characteristics,producing area and growth characteristics of sixteen new varieties of Chinese white poplar (Populus tomentosa Carr.) are reported,aiming at providing reference for breeding and extension of new varieties.     

冬小麦生长后期上三叶性状与抗旱性及产量构成因素的关系

试验利用20个旱地小麦品种,在育种圃种植模式下,对生长后期（挑旗期、抽穗期、半仁期、顶满仓期）上部三片叶的叶片含水量及其它性状与抗旱性和产量指标之间的关系,采用相关和回归分析方法进行分析,总结出不同时期上三叶性状与抗旱性和产量及其构成因素的关系。结果表明：在不同时期同一育种目标对上三叶的性状要求不同,同一时期不同叶片对抗旱性和产量及其构成因素的贡献也不同。叶片含水量和抗旱性之间直接效应不大;从整体上可以看出,上三叶越长、长/宽越大,抗旱性越强;旗叶叶片较窄且薄,倒二叶、倒三叶宽度较小是抗旱的理想类型。     

鸡滑液囊支原体感染的诊断与防控

旨在为鸡滑液囊支原体感染的检测、诊断和防控提供参考。通过查阅与鸡滑液囊支原体（MS）感染的检测、诊断和防控相关的文献资料发现：血清学检测方法操作简单、诊断快速，常作为MS感染的初步诊断方法，但其存在常出现非特异性交叉反应而影响诊断准确性的问题；分离培养MS病原的难度大、耗时长，不能快速做出诊断；分子生物学诊断方法快速、灵敏，有明显的优势，但技术条件要求较高。最终确诊需要多种诊断方法联合使用，可采用ELISA与PCR法检测，并结合临床症状和病理变化来判断鸡场MS的感染情况。通过采取净化种鸡群、加强饲养管理及生物安全管理、接种疫苗等措施可有效防控MS感染。     

规模化鸡场蛋壳质量的影响因素与调控措施

蛋壳的质量是评定鸡蛋品质的一项重要指标,蛋壳质量的好坏对蛋的运输、贮存、加工、消费和孵化都有极大的影响。就影响蛋壳质量因素的钙、磷、维生素、微量元素、电解质、鸡龄、饲养管理、疾病与药物等方面进行了阐述,并综述了提高蛋壳质量的调控措施,以期对广大鸡蛋生产者和经营者提供帮助。