不同海拔下甲醇替代率和主喷正时对RCCI发动机性能的影响

为探究不同海拔条件下甲醇/柴油反应活性控制压燃（reactivity controlled compression ignition, RCCI）发动机的运行特性,该研究基于甲醇/柴油双燃料发动机试验台架,试验研究1 800r/min、100%负荷和3 200r/min、100%负荷下不同甲醇替代率、柴油喷射正时对发动机燃烧与排放性能的影响规律。结果表明：不同海拔条件下随着甲醇替代率的增加,缸压和瞬时放热率峰值逐渐升高,燃烧始点和燃烧中心前移,当量有效燃油消耗率（equivalent brake specific fuel consumption, ESFC）降低,有效热效率升高,NO_x和碳烟排放大幅降低,THC（total hydrocarbons）和CO排放增加。1 800 r/min、100%负荷工况下,甲醇替代率由0增至20%,0、1 000、2 000m海拔下最大缸压平均增加1.72MPa,瞬时放热率峰值平均升高25.08J/(°),ESFC平均降低4.67%,有效热效率平均升高4.90%,NO_x和碳烟排放分别平均降低16.63%和50%,THC和CO排放量分别平均增加142.03、388.18 mg/kg。3 200 r/min下甲醇替代率由0增至7%,不同海拔高度下ESFC平均降低1.76%,有效热效率平均升高1.79%,NO_x和碳烟排放量分别平均降低8.17%和20.70%。海拔高度由0升至2 000m,1 800r/min、20%甲醇替代率与3 200r/min、7%甲醇替代率下,瞬时放热率峰值分别降低4.80和8.08J/(°),燃烧中心分别推迟1.44°和1.43°,有效热效率分别降低0.82%和0.68%,ESFC分别升高2.10%和1.99%,NO_x排放量分别减少10.61%和7.35%,碳烟排放分别增加26.54%和32.12%,THC排放分别升高29.88%和15.45%,CO排放量分别增加22.42%和18.15%。固定甲醇替代率后,随着柴油主喷正时提前,不同海拔条件下缸压和放热率峰值逐渐升高,燃烧中心向上止点靠近,ESFC逐渐降低,有效热效率升高,碳烟排放减少,NO_x、THC和CO排放增加。1 800 r/min、15%甲醇替代率下,主喷正时从-1.5°提前至-7.5°,不同海拔高度下ESFC平均降低8.27%,有效热效率平均升高9.08%,碳烟排放平均减少90.94%。为提升高海拔条件下甲醇/柴油RCCI发动机的热效率和燃油经济性,可以适当增大柴油主喷正时。研究结果可为不同海拔环境下甲醇/柴油RCCI发动机燃烧与污染物排放控制优化提供参考。     

温度和砷对不同品种水稻幼苗生长和砷吸收的影响

为明确温度和外源砷对水稻生长发育的影响,选取江苏地区常见的8个水稻品种为试验材料,通过添加不同浓度外源砷[0(As0)、0.5(As0.5) 和1 mg?L-1(As1)]和模拟不同温度[白天/夜晚分别为30 ℃/25 ℃（T0）和35 ℃/30 ℃（T1）],在人工气候箱内进行了发芽和苗期培养试验,并分析了8个品种水稻种子萌发、幼苗生长及砷含量状况。结果表明,外源砷对水稻的芽长和活力指数具有抑制作用,与对照（T0As0）相比,T0As1处理使不同品种水稻的芽长和活力指数分别降低13.69%~43.34%和28.14%~52.88%。 而温度对水稻种子萌发的影响与水稻品种有关。在T1处理下,盐两优1618的发芽率、芽长和活力指数均优于其他品种。温度和外源砷的共同作用显著降低了不同品种水稻的芽长（P<0.05）。与T0As0相比,T1As1使水稻芽长显著降低5.66%~43.34%。水稻根长和根系活力显著受到温度和外源砷的单一因素的影响。与T0As0相比,T0As1处理使水稻根系活力降低3.01%~58.21%。温度和外源砷的共同作用抑制了水稻根长和根系活力,其中T1As1使水稻根系活力显著降低53.80%~89.01%。不同品种水稻的苗高和根系活力在相同温度或外源砷处理下具有显著差异（P<0.05）,其中盐两优888的苗高和根系活力均处于较高水平。水稻茎叶砷含量在外源砷处理下显著增加,在增温处理下却降低。与单一的砷处理相比,温度和外源砷的共同作用降低了水稻茎叶的砷含量。综上可知,温度和外源砷影响水稻的生长及砷吸收,但水稻生长状况具有明显的品种间差异,其中盐两优888和盐两优1618在增温和外源砷共存条件下的种子萌发和生长状况优于其他水稻品种。     

60Co辐射诱变姬松茸突变株J3子实体不同部位的氨基酸分析研究

测定并比较了60Co辐射诱变姬松茸突变株J3与原菌株J1子实体不同部位的氨基酸含量.结果表明,除菌柄和盖皮外,姬松茸突变株J3子实体其它部位的必需氨基酸、儿童氨基酸、硫氨酸、支链氨基酸、鲜味氨基酸、芳香族氨基酸和甜味氨基酸均高于原菌株J1.姬松茸突变株J3子实体中谷氨酸含量占氨基酸总量的比例分别比原菌株J1、α-酪蛋白和卵清蛋白提高34.88%、56.68%和122.32%.     

Biological characteristics of long passaging rat mesenchymal stem cells

AIM:To investigate multi-potential of rat bone marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC were long passaged in vitro. METHODS:Cellular cycles of different passages were assayed by FACSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS:The early passages and long-term passages all showed strong proliferation; passage 6, passage 25 and passage 45 all showed normal karyotype. CONCLUSION:Long-term culture and passage of rBMMSC still remains strong proliferation. With this capability, the mutation inclination is not enhanced.     

Effects of FK506 on anti-glomerular basement membrane nephritis in rats

AIM:To investigate the effects of FK506 on anti-glomerular basement membrane(GBM) nephritis in rats. METHODS: Anti-GBM nephritis model was elaborated by rabbit anti-rat GBM antibody injection in SD rats in this study. The rats were divided into three groups: FK506 treated group(0.5 mg·kg^-1·d^-1, sc), untreated nephritis control group and normal control group. FK506 was administered daily six hours after injection of anti-GBM IgG. All the rats were observed urinary protein at the 4th day, the 14th day and the 21st day. At the same time, the kidney specimens were collected, and T cell transforming function was also monitored.RESULTS:Rats injected with rabbit anti-GBM Ab developed heavy proteinuria by 4 days, and serum creatinine and serum urea appeared which kept on the rising. Glmerular hypercellularity, crescents, and protein casts were observed in nephritic rats. By electron microscopy, the thickening of GBM and loss of foot processes were seen. T cell transforming function was higher than normal. But, all pathological changes obviously turned for the better in FK506 treated group. CONCLUSION:FK506 could inhibit the progression of rat anti-GBM nephritis.     

Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway

AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE₂ concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE₂ production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway.     

Effect of YIGU capsule on IGF-I mRNA and protein expression in rat osteoblasts in vitro

AIM: The effects of YIGU capsule on proliferation and IGF-I mRNA protein expressions in osteoblasts were studied. METHODS: (1) Forty 12-month old Sprague-Dawley female rats were divided randomly into four groups (YIGU capsule high dose group, medium dose group and low dose group; saline group), the drug-containing serum and control serum were prepared. (2) The new-born Sprague-Dawley rat osteoblasts were cultured with different YIGU capsule drug-containing serum at different concentrations and different exposure time. MTT method was used to observe proliferation of osteoblasts. (3) RT-PCR method was used to measure the relative IGF-I mRNA levels and ELISA method was used to measure IGF-I secretion at different exposure time. (4) ELISA method was used to measure IGF-I secretion at different exposure time. RESULTS: (1) Proliferation of osteoblasts was more than the control groups after 48, 72 and 96 h, respectively (P<0.01); (2) The relative IGF-I mRNA levels and IGF-I protein expression were higher than those in control group after 48, 72 and 96 h, respectively (P<0.01 or P<0.05). CONCLUSIONS: It was suggested that YIGU capsule drug-containing serum promoted proliferation, IGF-I mRNA and protein expression. These results may be parts of the mechanisms of YIGU capsule to prevent and treat osteoporosis.     

Recent advances in molecular mechanisms of oncosis

Oncosis is another form of cell death, which is different from apoptosis. The review will discuss the recent advances of oncosis on pathological morphology, nuclear biochemical changes and molecular mechanisms.