Effects of isorhapontigenin on cell cycle arrest and down-regulation of cyclin D1 expression in bladder cancer cells

AIM：To explore the inhibitory mechanism of isorhapontigenin (ISO) on the proliferation, migration and invasion of UMUC3 bladder cancer cells. METHODS：Human UMUC3 bladder cancer cells were pretreated with ISO, and the proliferation of the cells was observed under phase-contrast microscope and by ATPase assay. The expression of cyclin D1 was determined by RT-PCR and Western blotting. The cell cycle alteration was detected by flow cytometry, and the cell migration was examined by wound-healing assay. RESULTS：Over 20 μmol/L of ISO significantly inhibited the proliferation of UMUC3 cells with the IC50 of (22.5±2.8) μmol/L. The mRNA and protein levels of cyclin D1 in UMUC3 cells were markedly decreased after treatment with ISO. Exposure of UMUC3 cells to low dose (5 μmol/L) of ISO led to significant induction of G0/G1 growth arrest at both 12 h (58.82%) and 24 h (63.94%), compared with the negative control cells (47.33%) without inducing obvious apoptosis. ISO at dose of 5 μmol/L also markedly inhibited the cell migration. CONCLUSION：ISO significantly exhibits inhibitory effects on the proliferation and migration of human bladder cancer cells by down-regulation of cyclin D1 expression accompanying with G0/G1 cell cycle arrest.     

Immune responses in piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus

Porcine reproductive and respiratory syndrome virus (PRRSV) infection compromises the host's innate and adaptive immunity. The aim of this study was to investigate the immune responses of piglets infected with highly pathogenic (HP) PRRSV (HuN4 strain) with or without the immunization with CH-1R attenuated PRRSV vaccine. The response was evaluated for the clinical signs, pathological changes and virus load in immune organs, antibody responses and levels of serum IFN-γ, IL-4 and IL-10. The result showed that in comparison with the piglets received the immunization, the piglets infected with HP-PRRSV alone had the thymus atrophy, decreased serum levels of IL-4 and increased serum levels of IL-10 and INF-γ. These results suggest that elevated IL-10 levels at the early stage of the infection may enhance virus survival and delay the induction of protective immunity, while increased levels of IL-4 induce the effective immune responses and increase the animals' health status.     

一年两作地区玉米直播机械化技术可行性研究

在山东一年两作的玉米播种中多年来一直沿用了麦田套种的习惯，成为机械化技术的大面积高效率应用的一个障碍。本文在总结山东省各地实践验的基础上，从机械技术的发展、玉米品种的培育和气候变迁等方面研究了实行玉米直播机械化技术的可行性，以期对于适宜地区的玉米生产提供参考。     

河北省地衣新记录属

报道了采自河北省地衣5新记录属:孔文衣属(Opegrapha Humb.)、袋衣属(Hypogymnia(Nyl.)Nyl.)、黄茶渍属(Candelariella Muell.Arg.)、黄烛衣属(Candelaria Massal.)和盾衣属(Peltula Nyl.)。文中对5属地衣的生境、识别特征和地理分布特点进行了简要概述。     

奶牛杂交群体及其遗传评估的模拟研究

为验证在奶牛杂交群体中将品种组成和杂合度作为协变量的遗传评估理论,为奶牛杂交模式选择和杂交群体遗传评估等实际问题提供理论依据,结合我国奶业现状,本研究模拟了在低产荷斯坦牛群引入法系蒙贝利亚牛、德系西门塔尔牛进行杂交,采用随机交配和三品种轮回杂交模式、群体世代重叠17年的繁育过程.群体杂合度在第7年后趋于平衡,轮回杂交模式的群体杂合度高于随机交配.经过杂交,原中低产荷斯坦牛群体在产奶性能、生长性能方面都有进展,轮回杂交提高幅度相对略高.此外,利用品种效应和杂合度效应剖分杂种优势,采用单性状和多性状动物模型对模拟群体进行遗传评估,效果基本一致,都可以很好地用于杂交群体的遗传评估.结果表明,对荷斯坦牛中低产群体进行杂交,可实现生产性能和效益的提高,回归模型可用于多元群体的遗传评估.     

新疆边境跨界动物疫病传入风险因素调查

新疆地域辽阔,周边国家动物疫情形势复杂,随时有传入外来动物疫病的危险。调查结果表明新疆接壤国家多且边境线长,地理环境复杂,动物及动物产品贸易频繁,野生动物种类繁多,虽然边界线隔离设施较好,但仍然存在跨界动物疫病传入的风险因素,需要有针对性地强化边境防控措施。     

双向电泳结合蛋白质印迹技术分析猪囊尾蚴乳酸脱氢酶

为了研究猪囊尾蚴乳酸脱氢酶(LDH)的表达,试验选择四川省雅江县呷拉乡猪带绦虫病患者,给其口服槟榔-南瓜子驱虫,收集、制备虫卵悬液(8万个/mL),再给3头20日龄三元杂交乳猪猪灌胃虫卵悬液,每头1 mL,40 d后收集、制备猪囊尾蚴蛋白,进行双向电泳(2-DE)分析,将凝胶蛋白斑点转移至聚偏氟乙烯膜(PVDF膜),用自制的大鼠抗猪带绦虫LDH血清作为一抗、健康大鼠血清作为阴性对照进行蛋白质印迹(Western-blotting)分析。结果表明:双向电泳凝胶共检测到(207±9)个蛋白质斑点,相对分子质量(Mr)为14 400～94 000,等电点(pI)为3.0～10.0;试验组特异性抗原抗体阳性杂交斑点为1个,阴性对照未见阳性杂交斑点;将Western-blotting检测的抗原抗体阳性杂交斑点与原双向电泳凝胶斑点进行比对,找到对应蛋白斑点,经ImageMaster 2D Platinum 5.0软件分析后初步确定该蛋白斑点的pI/Mr为7.03/35 368,与猪带绦虫LDH的pI/Mr理论推导值接近,说明猪囊尾蚴表达LDH。     

草原和农田几种主要鼠种数量动态研究及预测

2007年4～10月,在内蒙古呼和浩特郊区和锡林郭勒草原分别对长爪沙鼠、黑线仓鼠、五趾跳鼠和布氏田鼠进行了种群数量动态和预测研究.根据2007年实地调查数据,利用已经建立的预测模型,分别对上述4种鼠2008年的数量作出预测:预测2008年上述两地长爪沙鼠数量均不多,不致形成危害;黑线仓鼠2008年春季捕获率为0.97±0.48%～1.49±0.59%,秋季捕获率为0.52%,也不会形成危害;2007年五趾跳鼠捕获率0.06%,预测2008年该鼠数量也不多,不会造成危害;锡林郭勒草原2007年布氏田鼠年捕获率为1.67%,预测2008年布氏田鼠数量仍处在低谷期,不会形成大面积危害,但局部地方,如锡林浩特市郊的草场春季可能增多,应作好防治的准备工作.建议建立草原鼠类数量长期监测点,开展预测预报,在鼠的数量上升之前采取主动防治措施,把鼠密度长期控制在危害阈值之下,实现草原鼠害的持续控制.     

饲料的科学贮存

1饲料原料(1)动物蛋白质类饲料。鱼粉、蚕蛹、肉骨粉、骨粉等动物蛋白质类饲料极易染菌、生虫,影响其营养价值。这类饲料一般用量不大,采用塑料袋贮存较好。为防止饲料受潮发热霉变,装好后应封严,置于干燥、通风处。保存期间要勤检查温度,如有发热现象要及时处理。