Inhibitory effect of AuNPs-Endostar on melanoma lung metastasis in mice

AIM: To observe the influence of gold nanoparticles combined with Endostar (AuNPs-Endostar) on the melanoma lung metastasis of mice and the underlying mechanism. METHODS: C57BL/6 mice (n=24) were selected for constructing the model of spontaneous lung metastasis of melanoma B16-F10 cells. Subsequently, the mice were randomly divided into Endostar group, AuNPs group, AuNPs-Endostar group and model group. After the formation of melanoma, the mice in each group were injected with different drugs through tail vein for 0.1 mL daily. After 9 d, the mice were narcotized for cutting the tumors in situ. After the operation, they were raised for 2 weeks before killed for obtaining the lung tissues to observe the situation of the metastasis. HE staining was utilized for observing the necrosis status of the tumors in situ, while immunostaining was applied for testing the expression of CD31, carbonic anhydrase-IX (CA-IX), vimentin and zonula occludens-1 (ZO-1) in the tumors. RESULTS: Compared with model group, the pulmonary metastasis in the groups with medical treatment was obviously reduced. In AuNPs-Endostar group, the metastasis inhibition rate was the highest, and the tumor necrosis was also decreased obviously, with the significant reduction of CD31, CA-IX and vimentin expression in the tumors and significant increase in ZO-1 expression. CONCLUSION: Compared with using Endostar or AuNPs alone, the combination of AuNPs with Endostar significantly improves the curative effect of inhibiting the pulmonary metastasis of melanoma in the mice. The mechanism may be related to reducing the tumor angiogenesis, norma-lizing the blood vessels and improving tumor hypoxia, thus inhibiting the tumor epithelial-mesenchymal transition, increasing the tight junctions between tumor cells and decreasing the invasiveness.     

内生菌与雷公藤细胞悬浮共培养的生理生化特性

将2株雷公藤内生真菌Fusarium oxysporum NS33与Penicillium steckii NS6、2株内生细菌Enterobacter cloacae sub sp LG3与Serratia marcescens LY1及其组合分别与雷公藤细胞悬浮共培养,对不同培养体系内雷公藤细胞的生长及其生理生化特征进行研究。结果显示,在共培养前期,与对照相比,接种单一内生菌株提高了细胞的干重,其中菌株NS6的促生效果最明显;而在共培养后期,无论是单一内生菌还是混合内生菌均对细胞生长具有抑制作用。内生真菌和菌株组合处理的培养液p H值有明显的升高,而内生细菌LY1则明显降低了培养液的p H值,其具有产酸性。另外,当雷公藤细胞同混合菌株共培养时,培养基中总糖消耗量是最大的,而接种单独菌株时则对细胞可溶性蛋白含量具有一定的提高作用。接种内生菌会影响雷公藤细胞的POD、CAT及SOD活性,与对照相比,接种单独菌株会更加提升POD与CAT的活性,而细胞MDA含量则明显下降。     

薄皮甜瓜新品种‘陕甜9号’

‘陕甜9号’是用‘E13’作母本,‘C20’作父本杂交育成优质抗病薄皮甜瓜新品种。植株生长势中强,果实短梨形,平均单果质量500 g,果皮光滑,黄白色,果肉浅绿至白色,中心可溶性固形物含量14.7%,耐贮运。全生育期85～100 d,果实发育期28～32 d,抗病、抗逆性较强。适应于黑龙江、河北、甘肃、新疆等地区保护地及露地栽培。     

Heme oxygenase-1 protects renal function in septic rats via influencing expression of thrombomodulin

AIM: To investigate the protective effect of heme oxygenase-1 (HO-1) on the kidney of septic rats and the influence of HO-1 on the expression of thrombomodulin (TM) in the kidney. METHODS: Sepsis in rats was developed with cecal ligation and puncture (CLP). The septic rats were randomly divided into sham group, CLP group, CLP+HO-1 inducer group and CLP+HO-1 inhibitor group (n=18). The plasma levels of creatinine (Cr), cystatin-C (Cys-C), carboxyhemoglobin (COHb), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and TM, and the changes of prothrombin time (PT) and activated partial thromboplastin time (APTT) in each group were measured. Histopathological examination was performed in the kidney. The expression of TM in the kidney tissue was detected by Western blot. RESULTS: Compared with sham group, significantly elevated plasma levels of Cr, Cys-C, TNF-α, IL-1β and TM (P<0.05), shortened PT and APTT (P<0.05), significantly increased microthrombus formation, and lowered TM expression in the kidney (P<0.05) of CLP group were observed. The administration of hemin lowered the plasma levels of Cr, Cys-C, TNF-α, IL-1β and TM (P<0.05), prolonged PT and APTT (P<0.05), attenuated microthrombus formation, and up-regulated the expression of TM in the kidney (P<0.05). In contrast, ZnPP had the opposite effects. CONCLUSION: HO-1 increases the expression of TM in the kidney and exerts anticoagulatory and antiinflammatory effects, thereby improving renal function in the septic rats.     

引黄灌区不同肥料类型和施肥技术对稻田氮磷流失的影响

为减少引黄灌区稻田退水导致的农业面源污染,寻找水稻稳产和环境友好的最佳结合点,研究了大田示范条件下不同肥料类型和施肥技术对稻田田面水和排水沟退水氮磷变化特征的影响。结果表明,与常规施肥处理比较,优化施肥和侧条施肥均可以显著降低稻田田面水和排水沟中TN质量浓度,其中以侧条施肥处理效果最好,田面水和排水沟中TN质量浓度仅为常规施肥的47.4%和34.3%,各施肥处理间田面水和排水沟中TP质量浓度变化差异不显著。优化施肥和侧条施肥处理在肥料减量的条件下水稻产量并没有降低,其中有机种植处理产量最低,相对产量仅为常规处理的73.5%。综合考虑水稻产量和环境因素,优化施肥和侧条施肥技术可以作为环境友好型施肥技术在引黄灌区进行推广。     

带分流叶片水泵水轮机转子强度及模态分析

以带分流叶片的水泵水轮机为研究对象,基于CFD与FSI方法分析其泵工况下转子的振动特性,对3种开度即小开度9.8°、最优开度17.5°和大开度24.8°下的流场进行数值模拟,将额定工况下的流场结果导入转子结构实现单向流固耦合,对带预应力的转子模型进行形变与等效应力分析.此外,还计算了转轮的干湿模态,对比分析了不同条件下转轮的振动特性.结果表明:不同开度对应的预应力下转轮形变与等效应力分布规律相似,转轮形变主要发生在上冠低压侧.按ND(节径数)在转轮圆周方向划分振动扇区描述振型规律时发现,各扇区内转轮上冠、下环低压侧的振幅较大.湿模态下水介质对转轮振型影响不大,但会明显降低转轮的振幅.预应力对转轮固有频率影响较小,可忽略不计,但在湿模态下,转轮固有频率会显著下降,这表明分析结构振动特性时应充分考虑水介质作用.     

保水材料对河套灌区土壤硝态氮和铵态氮动态变化的影响

为了研究内蒙古河套灌区农业化肥面源污染检测和治理措施,采用大田土壤淋溶试验,分析了河套灌区农田在保水材料处理下,玉米生育期内土壤硝态氮、铵态氮累积量及动态变化特征。结果表明,与CK相比,保水材料处理玉米苗期土壤NO_3~--N累积量平均提高20.49%,收获期平均降低13.98%;苗期土壤NH_4~+-N累积量平均提高35.21%,收获期平均降低28.93%。保水材料有效地抑制了氮素的淋溶损失,提高了氮素利用率,同时保证了玉米生育后期有效氮的供应,避免短时间内氮素的大量累积,在一定程度上减少了化肥面源污染,为农业面源污染治理提供有力措施。     

虚拟仪器技术在温室测控系统中的研究

利用虚拟仪器软件开发平台LabVIEW，对温室测控系统进行了虚拟化设计。此系统由温度传感器和土壤湿度传感器将温室的空气温度、土壤含水量变化转化为电压变化，然后用虚拟仪器对输入到数据采集控制卡的电压信号进行实时采集。数据以spreadsheet类型文件格式存储起来，同时进行初步统计分析，最后通过数据采集控制卡输出开关信号，控制电动机实现温室天窗的开启，以调节空气温度和控制电磁阀，实现喷水的控制，调节土壤湿度，同时将反馈信号显示在操作平台上。     

土壤及泥沙颗粒组成与养分流失的研究

利用自然降雨影响下的农田为试验样地,分析了降雨前后土壤颗粒组成与养分含量的变化,探讨了流失泥沙颗粒组成与养分的变化特征。结果表明,在降雨后,西瓜地与油菜地表土颗粒组成呈现粘粒与粉粒含量减少,砂粒含量相对增加的趋势;土壤养分含量都有不同程度的下降,其中水解氮与速效磷下降的幅度很大,西瓜地与油菜地水解氮降雨后降幅分别为9.67%和7.99%,速效磷的降幅分别为9.20%和8.76%;全氮养分降幅很小,西瓜地与油菜地分别为2.17%和1.54%;流失泥沙细小颗粒含量与养分含量随时间基本都呈降低的变化趋势,并且细小颗粒含量与携带流失的养分含量有显著甚至极显著的正相关性;流失泥沙具有富集粘粒和富集养分的特性,油菜地与西瓜地粘粒富集率分别为1.23和1.20,两样地全氮与速效磷的富集率都在1.3以上,而全磷和水解氮富集率基本处在1.1-1.3之间。     

大同市区主要蔬菜生产基地环境质量评价分级

对山西省大同市区主要蔬菜生产基地进行环境质量评价及其分级结果表明,该区主要蔬菜生产基地环境质量总体优良,完全满足目前国家所规定的无公害蔬菜或绿色食品产地环境质量的要求。