干旱沙区农田防护林营建模式与经营评价研究

通过对干旱沙区人工绿洲农田防护林林木生长量的调查测定和样本树干解析数据,揭示了林木生长规律,建立了树干材积模型,为农田防护林合理更新提出了科学依据。利用相关依据指标,对现有农田防护林的营建模式进行了评价,提出了新的更新模式。     

奶牛乳房炎金黄色葡萄球菌疫苗研究进展

奶牛乳房炎是影响乳品工业经济效益的重要疾病之一,引起奶牛乳房炎的致病菌约有150多种,但金黄色葡萄球菌是引起奶牛乳房炎的主要病原。由它引起的乳房炎很难治愈,而且对抗生素产生耐药性的菌株已经相当普遍,因此使用预防或治疗性疫苗防治乳房炎就成为首选。然而金黄色葡萄球菌的致病力由许多不同的因子(如黏附素、荚膜等)决定,这使得制备非常有效的疫苗变得很困难。本文就这些致病因子在疫苗研制方面的进展做一简要介绍。     

中药复方制剂“促免增重颗粒”对云岭黑山羊血液生理生化指标的影响

本研究旨在探讨中药复方制剂促免增重颗粒对云岭黑山羊血液生理生化指标的影响。选择6-12月龄云岭黑山羊40只,按照体重相近的原则随机分为4个组,每组10只。对照组饲喂基础日粮,促免增重颗粒组、四君子散组、强壮散组分别在精料中添加促免增重颗粒、四君子散和强壮散,预饲期为7 d,正试期为60 d。在正试期第0、30和60天采样,检测血液学和血液生化指标。结果显示,促免增重颗粒对云岭黑山羊血液生理生化指标有一定影响,表明促免增重颗粒有提高免疫功能的作用。     

唐山市耕地资源现状与可持续利用对策分析

唐山市是一个以重工业为主的老工业城市。近年来,由于城市化的发展以及其他不合理的开发利用,再加上水土流失、工业废物排放、农药化肥的大量使用、不合理的灌溉方式及自然灾害等直接影响了耕地资源的有效、持续利用,使得唐山市的耕地资源大量减少,质量严重下降。为此,就唐山市耕地资源出现的问题进行了初步探讨,分析了产生问题的原因,提出了耕地资源可持续利用的对策。     

毛乌素沙地臭柏、油蒿细根生产动态及影响因子

在毛乌素沙地开发整治研究中心北试实验地选取了七块样地,并依据建群植被外貌特征划分为臭柏群落、臭柏灌丛、油蒿群落三类。用根钻对各样地一个生长季内的根系进行了五次取样,同时测量土壤水分、枝条生长长度、温湿度等一些气象因子。对活细根分级并称干重,发现臭柏、油蒿不同径级细根的季节动态不同,不同土层内相同径级的细根生产动态也不相同:①臭柏与油蒿D≤2 mm的细根生物量在4~5月份都有一个快速增长的阶段,随后增长格局各不相同。②臭柏灌丛中,1 mm相似文献   

甘肃省生态承载力分析

近年来,随着经济的发展和人口的增加,水、土地等资源不足的矛盾日益突出,实现甘肃经济、社会和资源环境的可持续发展变得日益紧迫。本文以甘肃省实际情况为依据,通过建立区域生态承载力指标体系,利用主成分分析方法定量分析各个城市承载力情况,并在此基础上进行城市承载力类型划分,根据不同类型城市生态承载力相对优势和薄弱环节,对其今后的发展提出若干建议。     

Autophagy induces mitochondrial dysfunction in neurons from neonatal rats with hypoxic ischemic encephalopathy

AIM: To explore the influence of autophagy on the induction of mitochondrial dysfunction in the neurons in a neonatal rat hypoxic ischemic encephalopathy (HIE) model. METHODS: Ten-day-old rat pups (n=30) were randomly divided into sham group and model group. The rats in the latter group were subject to hypoxia-ischemia treatment via unilateral common carotid artery ligation. The rats were sacrificed for brain pathological examination, and the protein levels of cleaved caspase-3 and LC3B-II were detected by immunohistochemical analysis. For the in vitro experiments, the autophagy of primarily cultured rat neurons was observed after hypoxia, and Western blot and mitochondrial function testing were also performed. RESULTS: Compare with sham group, the hypoxia-ischemia treatment caused atrophy and apoptosis of neurons, and ventricular area enlargement of rat brains. Immunohistochemical results demonstrated significantly higher levels of apoptosis- and autophagy-associated proteins, such as cleaved caspase-3 and LC3B-II (P<0.01). In vitro experiments demonstrated that hypoxia induced autophagy and apoptosis in the neurons. Compared with sham group, there were higher levels of reactive oxygen species and mitochondrial superoxide, and lower mitochondrial membrane potential in the model group (P<0.01). CONCLUSION: In neonatal HIE rat model, the hypoxia-induced mitochondrial dysfunction is related to apoptosis and autophagy. It will provide a new idea for administration of autopahgy inducer agents in treatment of HIE.     

Effect of DNA methylation of miR-30a-5p promoter region on hepatic injury induced by homocysteine

AIM: To explore the role of DNA methylation of microRNA-30a-5p(miR-30a-5p) promoter region in hepatic injury. METHODS: Four-week-old normal mice and cystathionine β-synthase (CBS) single gene knockout mice were used and divided into normal (CBS^+/+, n=12) group and single gene knockout (CBS^+/-, n=12) group, and the mice were fed with high methionine diet for 8 weeks. HL-7702 hepatic cells were routinely cultured in vitro and divided into control group, homocysteine (Hcy) group and Hcy+5-azacytidne (AZC) group. Serum Hcy, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured by automatic biochemical analyzer. The levels of ALT and AST in the cells culture medium were determined by the microplate method. Hepatic injury in the mice were observed with HE staining. Cell viability staining was used to measure the viability of hepatocytes. RT-qPCR was used to detect the expression of miR-30a-5p in the liver tissues and hepatocytes. The correlation between the expression of miR-30a-5p and serum ALT and AST levels was analyzed by Pearson correlation analysis. DNA methylation level of miR-30a-5p promoter region in the liver tissues and hepatocytes was detected by nested landing methylation-specific PCR (nMS-PCR). RESULTS: Compared with the CBS^+/+ mice, the serum levels of Hcy, ALT and AST in the CBS^+/- mice were significantly increased (P < 0.05). HE staining showed the hepatocyte swelling and nuclear fragmentation and dissolution. The expression level of miR-30a-5p in the liver tissues was decreased (P < 0.01). Besides, the expression level of miR-30a-5p in the mice was negatively correlated with serum ALT and AST levels (r²=0.4557, P=0.0003, r²=0.4626, P=0.0003), and the DNA methylation of miR-30a-5p promoter region was increased (P < 0.01). In the HL-7702 cells, compared with control group,the ALT and AST levels were increased in Hcy group (P < 0.05, P < 0.01), and the cell viability was remarkablely decreased. DNA methylation of miR-30a-5p promoter region was increased (P < 0.01), which decreased after treated the cells with AZC (P < 0.05), while the expression level of miR-30a-5p in the cells was increased (P < 0.05). CONCLUSION: Hypermethylation of miR-30a-5p promoter region may play an important role in hepatic injury.     

文心兰新品种‘海文吉祥1号’

文心兰新品种‘海文吉祥1号’是由文心兰‘柠檬绿’（HoneyAngle）组培苗芽变株选育而来。花梗直立坚挺,花瓣和萼瓣淡黄色,唇瓣柱头处呈黄色,向四周边缘逐渐变浅成乳白色。为切花型品种,观赏价值高,应用前景广。     

A New Beam Column Element of Space Framed Structures for Nonlinear Analysis of Geometric Material

An incremental virtual work equation of three dimensional(3D) beam column element is proposed based on the UL method. The geometric nonlinear tangent stiffness matrix for 3D beam column element is deduced with the consideration of warping, shear deformation and effects of moment spatial rotation. Material nonlinearity is addressed by introducing plastic hinges at the element ends. And the Orbison sectional strength is modified in order to consider the effects of torsion moment and warping. The elastic plastic tangent stiffness matrix is acquired as the element ends are in plastic phase based on the flow theory. With a case studied, it is shown that the model can be used to predict accurately the ultimate bearing capacity and instability mode of 3Dspace frames by only one or two elements, and address the effects of warping and shear deformations effectively.