Effects of leaf age and tree size on stomatal and mesophyll limitations to photosynthesis in mountain beech (Nothofagus solandrii var. cliffortiodes)

Mesophyll conductance, g(m), was estimated from measurements of stomatal conductance to carbon dioxide transfer, g(s), photosynthesis, A, and chlorophyll fluorescence for Year 0 (current-year) and Year 1 (1-year-old) fully sunlit leaves from short (2 m tall, 10-year-old) and tall (15 m tall, 120-year-old) Nothofagus solandrii var. cliffortiodes trees growing in adjacent stands. Rates of photosynthesis at saturating irradiance and ambient CO(2) partial pressure, A(satQ), were 25% lower and maximum rates of carboxylation, V(cmax), were 44% lower in Year 1 leaves compared with Year 0 leaves across both tree sizes. Although g(s) and g(m) were not significantly different between Year 0 and Year 1 leaves and g(s) was not significantly different between tree heights, g(m) was significantly (19%) lower for leaves on tall trees compared with leaves on short trees. Overall, V(cmax) was 60% higher when expressed on the basis of CO(2) partial pressure at the chloroplasts, C(c), compared with V(cmax) on the basis of intercellular CO(2) partial pressure, C(i), but this varied with leaf age and tree size. To interpret the relative stomatal and mesophyll limitations to photosynthesis, we used a model of carbon isotopic composition for whole leaves incorporating g(m) effects to generate a surface of 'operating values' of A over the growing season for all leaf classes. Our analysis showed that A was slightly higher for leaves on short compared with tall trees, but lower g(m) apparently reduced actual A substantially compared with A(satQ). Our findings showed that lower rates of photosynthesis in Year 1 leaves compared with Year 0 leaves were attributable more to increased biochemical limitation to photosynthesis in Year 1 leaves than differences in g(m). However, lower A in leaves on tall trees compared with those on short trees could be attributed in part to lower g(m) and higher stomatal, L(s), and mesophyll, L(m), limitations to photosynthesis, consistent with steeper hydraulic gradients in tall trees.     

Emergence of Edwardsiella piscicida in Farmed Channel ♀, Ictalurus punctatus × Blue ♂, Ictalurus furcatus,Hybrid Catfish Cultured in Mississippi

There is a trend toward the increased incidence and prevalence of Edwardsiella piscicida septicemia in US catfish aquaculture, particularly in channel ♀, Ictalurus punctatus, × blue ♂, I. furcatus, hybrid catfish. From 2013 to 2017, a total of 3242 disease case submissions were made to the Aquatic Research and Diagnostic Laboratory (ARDL) at the Thad Cochran National Warmwater Aquaculture Center in Stoneville, MS. Of these, 1400 (43.2%) were hybrids. E. piscicida was suspected in 138 (4.3%) of cases, the majority of which (89.1%) were from hybrid catfish. A molecular survey of these isolates confirmed the majority (92.0%) to be E. piscicida. Furthermore, cases of E. piscicida from hybrids submitted to the ARDL and the Aquatic Diagnostic Laboratory of the Mississippi State University College of Veterinary Medicine in Starkville, MS, were documented for gross lesions and histological analysis. Grossly, E. piscicida presents with small dermal ulcerations, a raised fluid‐filled cranial midline lesion that is frequently ulcerated, hemorrhage in the gills, exophthalmia, and abdominal distension. Internally, lesions include splenomegaly, straw‐colored ascites, renomegaly, and occasionally hemorrhagic intestines. Histopathological examination is in agreement with gross observations, and infected fish repeatedly demonstrate a mononuclear meningoencephalitis, hemorrhagic branchitis, splenitis, ulcerative dermatitis, granulomatous interstitial nephritis, and hepatitis coupled with a hemorrhagic enteritis.     

An analysis of a community food waste stream

Food waste comprises a significant portion of the waste stream in industrialized countries, contributing to ecological damages and nutritional losses. Guided by a systems approach, this study quantified food waste in one U.S. County in 1998–1999. Publications and personal interviews were used to quantify waste from food production, processing, distribution, and consumption. Approximately 10,205 tons of food waste was generated annually in this community food system. Of all food waste, production waste comprised 20%, processing 1%, distribution 19%, and 60% of food waste was generated by consumers. Less than one-third (28%) of total food waste was recovered via composting (25%) and food donations (3%), and over 7,000 tons (72%) were landfilled. More than 8.8 billion kilocalories of food were wasted, enough to feed county residents for 1.5 months. This case study offers an example of procedures to quantify and compare food waste across a whole community food system.     

Evaluation of 2 sources of Angus cattle under South Florida subtropical conditions

The objective of this study was to compare performance and aspects of adaptability attributes of cattle from a Florida Angus bloodline (local source from a mostly closed herd for over 50 yr) to cattle that are representative of modern Angus bloodlines (outside source) in US subtropical conditions. Embryos from both sources were transferred to Brahman-crossbred cows in South Florida, and calves (n=82) were born in 3 yr. Before weaning, summer tympanic temperatures were recorded hourly for 3 d in each year. Heifers were placed with fertile bulls until diagnosed pregnant. Traits relative to sexual maturation of bulls were recorded at 1- or 2-mo intervals until approximately 17 mo of age. Calves from outside sources had greater hip height at weaning than calves from the local source (P<0.001; 108.8 ± 0.62 and 104.7 ± 0.68 cm, respectively). Local-source calves (n=37) had greater (P=0.03) exit velocity (2.7 ± 0.3 m/s) than outside-source (n=45) calves (2.0 ± 0.29 m/s), which may be indicative of more nervous or temperamental disposition. However, no source differences were detected for other assessments of disposition (chute or pen score, P>0.8). Few source differences for minimum, maximum, or range of daily tympanic (inner ear) temperatures were detected. At 17 mo of age, outside-source heifers were heavier (P = 0.05) and had greater (P<0.001) hip height than Angus heifers from the local source. Heifers from the outside source were younger (P<0.001) at the time of their first conception (454 ± 17.5 d) than heifers from the local source (550 ± 16.9 d). Outside-source heifers also had greater (P<0.02) pregnancy and calving rates (0.7 ± 0.119 and 0.62 ± 0.125, respectively) from exposure to bulls within a year from weaning than the heifers from the local source (0.29 ± 0.089 and 0.19 ± 0.077, respectively). Bulls from the outside source were heavier (P=0.05) at 320 d of age than local-source bulls. From 14 through 17 mo of age, outside-source bulls had greater (P≤0.05) scrotal circumference and tended (P≤0.15) to be heavier than local-source bulls. There appeared to be no performance or adaptation advantages for the local-source Angus through 17 mo of age. The large source difference for age at first conception in heifers merits additional attention and comparison with cow lifetime production performance for the 2 sources.     

Scaling foliar respiration to the stand level throughout the growing season in a Quercus rubra forest

Stand-level, canopy foliar carbon loss (R(can)) was modeled for a virtual Quercus rubra L. monoculture at two sites differing in soil water availability in a northeastern deciduous forest (USA) throughout the 2003 growing season. Previously reported foliar respiratory temperature responses of Q. rubra were used to parameterize a full distributed physiology model that estimates R(can) by integrating the effects of season, site and canopy position, and represents the best estimation of R(can). Model sensitivity to five simplified parameterization scenarios was tested, and a reasonable procedure of simplification was established. Neglecting effects of season, site or canopy position on respiration causes considerable relative error in R(can) estimation. By contrast, assuming a constant E(0) (a temperature response variable of the respiration model), or a constant night temperature (mean nighttime temperature) caused only a small relative error (< 10%) compared with the full model. From June 8 to October 28, 2003, modeled R(can) of the virtual Q. rubra monoculture was, on average, 45.3 mmol CO(2) m(-2) night(-1) on a ground-area basis (or 334 mmol CO(2) kg(-1) night(-1) on a biomass basis) and 101 mmol CO(2) m(-2) night(-1) (or 361 mmol CO(2) kg(-1) night(-1)) at the drier site and the more mesic site, respectively. To model R(can) of Q. rubra (or other Quercus species with similar respiratory properties), variations in the base respiration rate across season, site and canopy position need to be fully accounted for, but E(0) may be assumed constant. Modeling R(can) at the mean nighttime temperature would not strongly affect estimated canopy carbon loss.     

Reactions of singlet oxygen with pine pollen

Exposure of pine pollen to single oxygen, generated in an aqueous environment, resulted in a decrease in the relative quantities of unsaturated fatty acids that could be recovered by solvent extraction of surface and near surface pollen lipids. The involvement of excited oxygen was confirmed by substitution of deuterium oxide for water, which led to a twofold greater decrease in the unsaturated acids. The potential environmental and biomedical implications of these observations are discussed in terms of this model system.