Environmental factors and megafauna spatio‐temporal co‐occurrence with purse‐seine fisheries

Tropical tuna purse‐seine fisheries spatially co‐occur with various megafauna species, such as whale sharks, dolphins and baleen whales in all oceans of the world. Here, we analyzed a 10‐year (2002–2011) dataset from logbooks of European tropical tuna purse‐seine vessels operating in the tropical Eastern Atlantic and Western Indian Oceans, with the aim of identifying the principle environmental variables under which such co‐occurrence appear. We applied a Delta‐model approach using Generalized Additive Models (GAM) and Boosted Regression Trees (BRT) models, accounting for spatial autocorrelation using a contiguity matrix based on a residuals autocovariate (RAC) approach. The variables that contributed most in the models were chlorophyll‐a concentration in the Atlantic Ocean, as well as depth and monsoon in the Indian Ocean. High co‐occurrence between whale sharks, baleen whales and tuna purse‐seine fisheries were mostly observed in productive areas during particular seasons. In light of the lack of a full coverage scientific observer on board program, the large, long‐term dataset obtained from logbooks of tuna purse‐seine vessels is highly important for identifying seasonal and spatial co‐occurrence between the distribution of fisheries and megafauna, and the underlying environmental variables. This study can help to design conservation management measures for megafauna species within the framework of spatial fishery management strategies.     

Long-term immunogenicity and protection against Mycoplasma agalactiae induced by an oil adjuvant vaccine in sheep

The long-term protective immunity of an inactivated mineral-oil adjuvanted Mycoplasma agalactiae vaccine was evaluated in sheep. The antigen suspension was emulsified with a mixture of three mineral oils (Montanide ISA-563, Marcol-52, Montane-80 at the ratio of 30%, 63%, and 7%, respectively). Twenty-two animals were divided in 2 groups (A and B) and immunised with two doses of the vaccine (group A, n = 14) or used as unvaccinated control (group B, n = 8). Five months after the second vaccination, seven animals of group A and four animals of group B were challenged by nasal route with M. agalactiae. The remaining seven vaccinated and four control animals were challenged intranasally eight months after vaccination. The vaccine was able to induce a full-protective immunity preventing the clinical signs of contagious agalactia and the infection by M. agalactiae in all groups of animals irrespective of the time of challenge after booster administration.     

Effect of carbohydrates on in vitro low-temperature storage of shoot cultures of apricot

Procedures for cold storage of in vitro cultures can delay subculturing, reducing production costs and risks of contamination and somaclonal variation. The present work investigates the effects of media with sorbitol (116.8 mM, medium SO) or sucrose (58.4 mM) alone (medium SU), or the latter in combination with mannitol (58.4 mM, medium M) on 7-month storage at 5 °C of apricot shoots, cv San Castrese and Boreale. Shoots in SO survived in lower percentages and grew less than in the other treatments during storage, and died in large numbers after transfer to standard culture conditions. In comparison to other treatments, survival was 100% in the presence of M and both shoot weight and number of surviving proliferated axillary shoots was increased. Moreover, M improved regrowth compared to SU under standard culture conditions. The SOD and CAT activity confirmed the higher stress of shoots stored in SO than controls, and in contrast, the low stress of shoots in M.     

Integrated approach of multiple environmental datasets for the assessment of sediment contamination in marine areas affected by long-lasting industrial activity: the case study of Bagnoli (southern Italy)

Purpose

The Bagnoli’s brownfield site (southern Italy) is an environmental priority for the Italian Government and is currently considered as a case study for the definition and application of innovative approaches to ecosystem recovery after a prolonged pollution history and eventual steel plant decommissioning.

Materials and methods

Here, we analyse high-quality datasets that constrain the distribution patterns and sources of contaminants in the coastal zone facing the site where the steel plant operated for almost one century. Innovative statistical approaches provide new insights into the temporal and spatial distribution of anthropogenic impacts, documented over the last 30 years, following lines of evidence emerging from investigations on grain size, TOC, heavy metals, PAHs, PCBs and high-resolution morpho-bathymetric information.

Results and discussion

The results derived from statistical analysis, referred also to the seafloor morphological variability, highlighted the presence of recent disposal of highly contaminated sediments and their potential pathways of dispersion to wider and deeper water zones.

Conclusions

The adopted multidisciplinary approach yields fundamental information towards an effective and proper ecosystem recovery of highly contaminated marine coastal areas and provides at the same time, a sound base for the refitting of decommissioned industrial plants.

     

Effect of menadione sodium bisulfite,an inducer of plant defenses,on the dynamic of banana phytoalexin accumulation during pathogenesis

Using an authentic sample of 2-hydroxy-9-(p-hydroxyphenyl)-phenalen-1-one, a banana phenalenone-type phytoalexin, we studied its dynamic of accumulation during pathogenesis of banana plants (Musa acuminata (AAA), Grand Nain) inoculated with Fusarium oxysporum f.sp. cubense (FOC), Race 4, the causal agent of Panama disease. The results obtained demonstrate that banana plants treated prior inoculation with menadione sodium bisulfite (MSB), an inducer of plant defenses, are capable of changing the dynamic of accumulation (higher amount and speed of biosynthesis) of this banana phytoalexin, biosynthesized by the banana plant during pathogenesis.     

From waste to resource: mycoremediation of contaminated marine sediments in the SEDITERRA Project

Purpose

Port-dredging activities produce large volumes of contaminated sediments. Dredged sediments are considered a waste by national laws, but recently the desire to consider them a resource has become widespread and remedies for their contamination are being searched to allow their reuse. In this work, we studied, developed, and tested a method for remediate marine-dredged sediments contaminated by heavy metals using native fungi and a microporous membrane, in order to achieve the sediment quality and allow their reuse.

Materials and methods

Activity was carried out on port sediments from Genoa, Leghorn, Pisa, and Cagliari (Italy). Autochthonous fungi were isolated from each sediment and employed in mycoremediation tests. Two plastic boxes were prepared (for each Port) with 5 kg of sediment in each box, employed for metal bioaccumulation using a sterile polyester membrane inoculated with fungi. Membranes were analyzed at 15, 30, and 60 days after inoculums, and sediments were analyzed after 60 days at the end of the experiment to verify metal contamination degree. Recovery efficiency (RE%) and difference recovery efficiency (DRE%) were calculated for each experiment: the first shows the absorption capability of the membrane-fungi consortium; the second evidences only the fungal contribution to the metal absorption. To assess sediment contamination before and after the mycoremediation treatment, we considered chemical levels of reference L1 (the lowest chemical level of reference) and L2 (the highest chemical level of reference), and the evaluation of chemical hazard (HQ) for the chemical contaminants defined by the Italian Ministerial Decree 173/2016.

Results and discussion

Fungi from Genoa sediments increase the membrane absorption of Cu and Zn. Regarding Leghorn results, RE (%) increases and reaches the maximum value after 60 days of treatment for each considered metal. Cr tot, Ni, and Mn appear to be hyper-bioaccumulated. DRE values of Pisa sediments show that Mn is excluded by fungi and it does not bioaccumulate, while other metals and in particular Cd, Cr tot, Zn, and Sb are bioaccumulated. Cagliari DREs show that fungi are not able to bioaccumulate Cr tot, Ni, and Mn and their accumulation is due to the membrane, while As and Cd are bioaccumulated.

Conclusions

Our work evidenced that selected fungi are able to grow on a microporous support and actively reduce metal concentrations in the sediments, achieving their quality. This biomembrane system may represent an important instrument for the remediation of the residual metal contamination of port sediments.

     

Therapeutic Ultrasound as a Potential Male Dog Contraceptive: Determination of the Most Effective Application Protocol

Ultrasound is one of the most promising forms of non‐invasive contraception and has been studied in several animal models. The objective of the current investigation was to determine the most practical and effective application protocol for dog sterilization. A total of 100 dogs were divided into five equal groups. Group A received 5‐min applications three times performed at 48‐hr intervals and covering the entire testicular area at frequency of 1 MHz; Group B received 5‐min applications three times performed at 48‐hr intervals over the dorso‐cranial area of the testis at frequency of 3 MHz; Group C received three sequential 5‐min applications (at 5‐min intervals between applications) covering the entire testicular area at frequency of 1 MHz; Group D received 15‐min applications two times performed at 48‐hr intervals and covering the entire testicular area at frequency of 1 MHz. The experimental groups' ultrasound had an intensity of 1.5W/cm². The Control Group had the same procedure as Group A, but with the transducer switched‐off. Dogs were surgically castrated 40 days following the treatment for histological examination. Azoospermia, testicular volume reduction and apparently irreversible testicular damage were achieved by Group A. No effects were noticed in the other groups. Testosterone levels remained within physiological range with all application protocols. A regimen of three applications of ultrasound at 1 MHz, and 1.5 W/cm², lasting 5 min with an interval of 48 h was effective as permanent sterilization in the dog without hormonal impact.     

NMR analysis of seven selections of vermentino grape berry: metabolites composition and development

The goal of this work was to study via NMR the unaltered metabolic profile of Sardinian Vermentino grape berry. Seven selections of Vermentino were harvested from the same vineyard. Berries were stored and extracted following an unbiased extraction protocol. Extracts were analyzed to investigate variability in metabolites concentration as a function of the clone, the position of berries in the bunch or growing area within the vineyard. Quantitative NMR and statistical analysis (PCA, correlation analysis, Anova) of the experimental data point out that, among the investigated sources of variation, the position of the berries within the bunch mainly influences the metabolic profile of berries, while the metabolic profile does not seem to be significantly influenced by growing area and clone. Significant variability of the amino acids such as arginine, proline, and organic acids (malic and citric) characterizes the rapid rearrangements of the metabolic profile in response to environmental stimuli. Finally, an application is described on the analysis of metabolite variation throughout the physiological development of berries.     

Chromatographic methods for metabolite profiling of virus- and phytoplasma-infected plants of Echinacea purpurea

This study was focused on the effects of virus and phytoplasma infections on the production of Echinacea purpurea (L.) Moench secondary metabolites, such as caffeic acid derivatives, alkamides, and essential oil. The identification of caffeic acid derivatives and alkamides was carried out by means of high-performance liquid chromatography-diode array detection (HPLC-DAD), HPLC-electrospray ionization-mass spectrometry (ESI-MS), and MS(2). Quantitative analysis of these compounds was carried out using HPLC-DAD. The results indicated that the presence of the two pathogens significantly decreases (P < 0.05) the content of cichoric acid, the main caffeic acid derivative. Regarding the main alkamide, dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide, a significant decrease (P < 0.05) in the content of this secondary metabolite was observed in virus-infected plants in comparison with healthy plants, while in the phytoplasma-infected sample the variation of this secondary metabolite was not appreciable. The % relative area of the E/Z isomers of this alkamide was also found to change in infected samples. The gas chromatography (GC) and GC-MS analysis of E. purpurea essential oil enabled the identification of 30 compounds. The main significant differences (P < 0.05) in the semiquantitative composition were observed for three components: limonene, cis-verbenol, and verbenone. The results indicate that the presence of virus and phytoplasma has an appreciable influence on the content of E. purpurea secondary metabolites, which is an important issue in defining the commercial quality, market value, and therapeutic efficacy of this herbal drug.