High frequency plant regeneration through adventitious multiple shoot organogenesis in epicotyl explants of Indian gooseberry (Emblica officinalis Gaertn)

Shoots regenerated adventitiously on epicotyl segments from in vitro seedlings of Emblica officinalis var. ‘Kanchan’. Epicotyls derived from 2-week-old aseptic seedlings were most responsive and produced a maximum number of 303 shoots per explant in Murashige and Skoog (1962) medium (MS) augmented with 8.8 μM N⁶-benzyladenine (BA) + 1.425 μM indole-3-acetic acid (IAA). Shoots readily elongated in MS lacking growth regulators and rooted in half-salt-strength MS (1/2 MS) supplemented with indole-3-butyric acid (IBA) or α-naphthalene acetic acid (NAA). The highest rooting response was recorded in 1/2 MS containing 14.7 μM IBA. Plantlets were acclimatized inside the green house and 80% of the plantlets survived on transfer to garden soil.     

Genotype × environment interaction for zinc and iron concentration of wheat grain in eastern Gangetic plains of India

Zinc and iron are important micronutrients for human health for which widespread deficiency occurs in many regions of the world including South Asia. Breeding efforts for enriching wheat grains with more zinc and iron are in progress in India, Pakistan and CIMMYT (International Maize and Wheat Improvement Centre). Further knowledge on genotype × environment interaction of these nutrients in the grain is expected to contribute to better understand the magnitude of this interaction and the potential identification of more stable genotypes for this trait. Elite lines from CIMMYT were evaluated in a multilocation trial in the eastern Gangetic plains (EGP) of India to determine genotype × environment (GE) interactions for agronomic and nutrient traits. Agronomic (yield and days to heading) data were available for 14 environments, while zinc and iron concentration of grains for 10 environments. Soil and meteorological data of each of the locations were also used. GE was significant for all the four traits. Locations showed contrasting response to grain iron and zinc. Compared to iron, zinc showed greater variation across locations. Maximum temperature was the major determinant for the four traits. Zinc content in 30–60 cm soil depth was also a significant determinant for grain zinc as well as iron concentration. The results suggest that the GE was substantial for grain iron and zinc and established varieties of eastern Gangetic plains India are not inferior to the CIMMYT germplasm tested. Hence, greater efforts taking care of GE interactions are needed to breed iron and zinc rich wheat lines.     

Evaluation of a recombinant LigB protein of Leptospira interrogans serovar Canicola in an enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis

A recombinant leptospiral lipoprotein, LigB, was evaluated for use in the diagnosis of bovine leptospirosis by enzyme-linked immunosorbent assay (rLigB IgG ELISA). The standard reference test (Microscopic agglutination test, MAT) of 200 serum samples from cattle suspected of leptospirosis showed that 95 (47.5%) samples had positive agglutination titres, which ranged from 100 to 1600. In rLigB IgG ELISA, 49% of the samples were positive. Sensitivity of IgG ELISA for 95 bovine sera, which had MAT titres of greater than or equal to 100, were 100%. ELISA showed a specificity of 97.1% with 105 bovine sera, which were negative at a 1:50 dilution in MAT for Leptospira interrogans serovars. The results of ELISA and MAT correspond very good. When analytical specificity of IgG ELISA was evaluated using bovine serum samples from animals showing the serum antibodies to other pathogens, no cross-reaction was observed. Thus the recombinant LigB IgG ELISA can be used instead of the MAT as an aid to the diagnosis of bovine leptospirosis.     

Nutrient Indexing in “‘Kinnow” Mandarin (Citrus deliciosia Lour.× Citrus nobilis Tanaka) Grown in Indogangetic Plains

Diagnosis and remediation of nutrient constraints in perennial fruit crop like citrus are the two important pillars of an effective nutrient management program. Efforts were made to develop nutrient indexing (NI) criteria based on generated leaf and soil analysis dataset for “Kinnow” mandarin (Citrus deliciosia Lour. × Citrus nobilis Tanaka) grown on illitic soils of Indogangetic plains (Entisol, Inceptisol, and Aridisol). NI through diagnosis and recommendation integrated system (DRIS) using leaf analysis data showed optimum value of leaf nutrient concentration as 2.22–2.32% nitrogen (N), 0.11–0.15% phosphorus (P), 1.10–1.41% potassium (K), 2.32–2.79% calcium (Ca), 0.38–0.61% magnesium (Mg), 22.4–58.3 ppm iron (Fe), 26.3–56.2 ppm manganese (Mn), 4.2–7.2 ppm copper (Cu), and 21.3–26.9 ppm zinc (Zn) vis-à-vis a fruit yield of 32.4–56.1 kg tree^?1. Using these NI criteria, Zn was observed as most deficient (64.7%) followed by Fe (61.5%), Mn (57.6%), N (96.1%), and P (38.5%) using percentage of orchards as basis. While, optimum NI (mg kg^?1) using soil analysis data was determined as 114.3–121.2 potassium permanganate (KMnO₄-N), 7.8–12.3 Olsen-P, 96.4–131.3 ammonium acetate (NH₄OAc)-K, 189.4–248.6 NH₄OAc-Ca, 72.3–89.9 NH₄OAc-Mg, 5.8–11.1, DTPA-Fe, 4.3–6.9 diethylenetriaminepentaacetic acid (DTPA)-Mn, 0.45–0.69 DTPA-Cu, and 21.3–26.9 DTPA-Zn for the optimum yield of 32.4–56.1 kg tree^?1. Soil analysis-based NIs displayed a good complementary with leaf analysis-based NIs evident from the diagnoses indicating Mn (52.2%) as most dominant constraint Zn (61.2%) followed by Mn (48.3%), N (41.2%), and P (35.6%). The recommended DRIS-based NIs would lay a scientific basis in formulating citrus fertilization program.