Clonal diversity of Staphylococcus aureus originating from the small ruminants goats and sheep

Staphylococcus aureus is an important pathogen in humans and many animal species. The prevalence of different clonal types in animal species remains largely unknown. We analyzed 267 S. aureus from intramammary infections in goats (47) and sheep (220) by spa typing, multi-locus sequence typing (MLST) and antimicrobial susceptibility. The most frequent spa types in goats were t337 (N=9), t759 (N=6) and t1534 (N=5). Sheep isolates mainly belonged to spa types t1534 (N=72), t2678 (N=29) and t3576 (N=20). Eighteen novel spa-types were observed; two from goat strains, 13 from sheep and three in both species. The majority of the goat strains grouped in MLST CC133 (N=10) and ST522 (N=10), followed by CC9 (N=9), while the majority of the sheep strains were of ST522 (N=108) followed by CC133 (N=86) and CC130 (N=11). Nine new MLST types were detected; three in goat and sheep isolates (ST1739, ST1758 and ST1780), two identified in goats only (ST1740 and ST2061) and four in sheep only (ST1742, ST1743, ST1781 and ST2011). Strains showed resistance below 20% against penicillin and tetracycline; a strong association between CC-types and penicillin resistance was observed. No resistance was detected to cefoxitin, quinupristin-dalfopristin, rifampicin and vancomycin. This study suggests that ST522 is the most common S. aureus clone associated with small ruminants followed by CC133.     

Hydroponic horticulture using residual waters from Oreochromis niloticus aquaculture with biofloc technology in photoautotrophic conditions with Chlorella microalgae

Protein‐dependent aquaculture generates large amounts of nutrient‐rich residuals; a feasible way to develop sustainable production systems is to integrate Decoupled Aquaponic Systems (DAPS) with residual water bioprocesses, to combine Photoautotrophic Biofloc Technology (P‐BFT) aquaculture and hydroponic horticulture. This study describes the characteristics of residual water from Oreochromis niloticus aquaculture performed with P‐BFT inoculated with Chlorella microalgae, reared during the nursery (180 fish m³) and grow‐out (55 fish m³) phases. The experiment included five treatments: photoautotrophic BFT inoculated with Chlorella sp. (M), C. sorokiniana 2714 (CV), and C. sorokiniana 2805 (CS), and chemoautotrophic (Q) and heterotrophic (H) as controls. Elemental characteristics in liquid and solid residual fractions (15 macro‐ and micronutrients) were compared among treatments and against Hoagland & Arnon solution with hydroponics and used in Nutrient Film Technique (NFT) hydroponic horticulture including five plant species: lettuce (Lactuca sativa), pak‐choi (Brassica rapa subsp. chinensis), rocket (Eruca sativa), spinach (Spinacia oleracea) and basil (Ocimum basilicum). The physicochemical parameters were ideal for O. niloticus and plants. The relationship between N:P was ideal until weeks 16–22 in the photoautotrophic treatments, compared with hydroponic solutions. Micronutrient content was greater in the solid than a liquid fraction. The best BFT effluent regarding fish and plant growth was photoautotrophic treatments. Oreochromis niloticus BFT aquaculture in photoautotrophic mode using microalgae Chlorella inoculations provided residual water beneficial to hydroponic horticulture in DAPS located in coastal arid zones where freshwater is scarce, improving aquaculture performance and reusing water and nutrients.     

Gradual Fall of s-Triazine Herbicides in Drinking and Wastewater Samples as Influenced by Light and Temperature

The effect of light and temperature on the dissipation of four s-triazine herbicides (terbuthylazine, simazine, atrazine and prometryn) was studied in drinking and wastewaters during long-term laboratory incubation (4 months), and by comparing the results with those obtained in purified water (Milli-Q). Residues were analyzed by GC-NPD and confirmed by GC-MSD. A micro on-line method for isolating the herbicide residues was used. The results showed that temperature and light had a certain influence on the behaviour of the s-triazine herbicides. In drinking water, prometryn dissipated more rapidly than the other compounds under all laboratory conditions (t _1/2= 75–128 days), while atrazine showed the highest degree of persistence (t _1/2= 132–227 days). In general, atrazine was the least (t _1/2= 90–142 days) and simazine the most (t _1/2= 118–278 days) persistent compound in Milli-Q and wastewater. Only in the case of atrazine in drinking water was the remaining percentage at the end of the experiment higher than 50% (53–69%). Dissolved organic substances in wastewater (DOC, 53.3 mg l^–1) appear to be particularly important in the photosensitization process. The time required to reach the maximum admissible concentration (MAC) according to European legislation (0.1 g l^–1) ranged from 3 to 10 years for prometryn and atrazine, respectively, in drinking water depending on the used conditions.     

Varietal influence of orange trees on armoured scale insect fecundity (Hemiptera: Diaspididae)

Abstract

We studied the influence of two orange tree varieties on the fecundity of two hemipteran diaspidid scale insect species: Cornuaspis beckii (Newman) and Parlatoria pergandii Comstock. Samples of females were taken at 7 – 40 day intervals from two orange orchards [Citrus sinensis (L.) Osbeck], one of the Navel Lane Late cultivar and the other of the Valencia Late cultivar. Different categories for the egg stage were considered: preoviposited, oviposited and hatched eggs. Average fecundity in C. beckii was significantly higher than that of P. pergandii. Significant differences between the two orange varieties were evident for some egg categories, with a higher fecundity in Navel Lane Late than in Valencia Late. The temporal pattern of fecundity in relation to accumulated temperature was studied by two methods: (i) comparing the parameter r of a logistic function that related egg-days to accumulated degree-days, and (ii) comparing the parameters b and c of a sinusoidal function that relates the variation egg-days/degree-days to accumulated degree-days. The comparison of such parameters (indicators of changes in the temporal patterns of fecundity) did not show significant differences between orange varieties. Our results suggest that some type of physiological difference between Navel Lane Late and Valencia varieties could be responsible for the differential fecundity of diaspines. Nevertheless, currently we cannot recommend changes in the management of diaspine in citrus orchards.     

Conservation status of the inland aquatic crustaceans in the Yucatan Peninsula,Mexico: shortcomings of a protection strategy

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Limited performance of MALDI‐TOF for identification of fish Aeromonas isolates at species level

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Use of an acoustic telemetry array for fine scale fish behaviour assessment of captive Paiche,Arapaima gigas,breeders

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Health risk behaviors and work injury among hispanic adolescents and farmworkers

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