Production of Surface-Active Compounds by a Hydrocarbon-Degrading Actinobacterium: Presumptive Relationship with Lipase Activity

The replacement of synthetic surface-active compounds (SACs) by their microbial counterparts is carving out a niche for themselves in the field of bioremediation. However, the high cost of microbial products has limited their application at a realistic scale. In the current study, several hydrocarbon-degrading microorganisms were assayed as potential SAC producers in low-cost liquid media. Only the strain CC10, placed within the class Actinobacteria, was able to produce emulsifying molecules by using a combination of sugarcane vinasse or crude glycerol (as cheap carbon substrates) with urea or peptone (as nitrogen sources). The emulsifying activity of the supernatants and the stability of emulsions formed with motor oil depended on the carbon and nitrogen sources. However, the biodegradability of these metabolites was only associated with the carbon substrate, and it was always higher than the two tested synthetic surfactants, sodium dodecyl sulfate and Triton X-100. Also, a positive linear association between emulsifying and lipase activities of the CC10 supernatants was detected (r?=?0.781; p?=?0.219), with the maximum activities detected in the glycerol-peptone supernatant. Interestingly, this supernatant was able to emulsify different oily substrates, a property that could be used to increase the efficiency of the treatment of effluents with high fat content.     

Can mineral and organic fertilization help sequestrate carbon dioxide in cropland?

The soil organic matter content represents a huge reservoir of plant nutrients and an effective safeguard against pollution; beside it can sequestrate atmospheric CO₂. Since 1966 up to now in the Southeast Po valley (Italy), the soil organic C (SOC) and total N (TN) dynamics in the 0–0.40 m soil layer under a maize–wheat rainfed rotation are studied as influenced by organic and mineral N fertilizations. Every year in the same plots cattle manure, cattle slurry, and crop residues (i.e. wheat straw and maize stalk) are ploughed under to 0.40 m depth at a same dry matter rate (6.0 and 7.5 t DM ha⁻¹ year⁻¹ after wheat and maize, respectively) and are compared to an unamended control. Each plot is splitted to receive four rates of mineral fertilizer (0–100–200–300 kg N ha⁻¹). In the whole experiment, in 2000 SOC concentration was lower than in 1966 (6.77 and 7.72 g kg⁻¹, respectively), likely for the deeper tillage that diluted SOC and favoured mineralization in deeper soil layer. From 1972 to 2000 SOC stock did not change in the control and N fertilized plots, while it increased at mean rates of 0.16, 0.18, and 0.26 t ha⁻¹ year⁻¹ with the incorporation of residues, slurry and manure, corresponding to sequestration efficiencies of 3.7, 3.8 and 8.1% of added C with the various materials. TN followed the same SOC dynamic, demonstrating how it depends on the soil organic matter. Manure thus confirmed its efficacy in increasing both SOC content and soil fertility on the long-term. In developed countries, however, this material has become scarcely available; slurry management is expensive and implies high environmental risks. Moreover, in a C balance at a farm (or regional) scale, the CO₂ lost during manure and slurry stocking should be considered. For these reasons, the incorporation of cereal residues, even if only a little of their C content was found capable of soil accumulation, appears the best way to obtain a significant CO₂ sequestration in developed countries. Our long-term experiment clearly shows how difficult it is to modify SOC content. Moreover, because climate and soil type can greatly influence SOC dynamic, to increase CO₂ sequestration in cropland, it is important to optimize the fertilization within an agricultural management that includes all the agronomic practices (e.g. tillage, water management, cover crops, etc.) favouring the organic matter build up in the soil.     

Chemical and biological diversity of Bergamot (Citrus bergamia) in relation to environmental factors

Oil of bergamot is receiving renewed popularity in aromatherapy. The biovariability of Citrus bergamia grown wild in Calabria (Italy) was investigated as far as chemical markers (linalool, linalyl acetate and bergapten) content and antioxidant and antifungal activities of the methanolic extracts. The average content in the markers presents slight variations with the altitude and more evident changes with the latitude of the areas of plant collection.     

Variability in the content of active constituents and biological activity of Glycyrrhiza glabra

Nine samples of Glycyrrhiza glabra were collected in various sites of Calabria, Italy, with the aim to determine the variability in the confront of active constituents and in antibacterial and antifungal activities of the extracts. The samples showed remarkable differences in chemical composition and biological activity.     

In vitro digestion assay for determination of hidden fumonisins in maize

Hidden fumonisins have received great attention in the last years as they have been frequently found in maize products in addition to the free forms. Several papers have shown that interaction with macromolecular components such as protein and starch is at the base of the phenomenon: although the nature of the interaction (covalent or not) is still not clarified, the occurrence of hidden forms is generally revealed by the application of an alkaline hydrolysis procedure. In this study, an in vitro digestion model has been applied to raw maize to evaluate the possible release of hidden fumonisins under gastrointestinal conditions. Upon digestion of the food matrix, an increased amount of total detectable fumonisins was observed in comparison with the analysis on the nondigested matrix, an amount even higher than that calculated through the application of the hydrolysis procedure. Besides the analytical issues, our data have serious implications, since consumers may be exposed to a systematic higher risk than that estimated by conventional techniques.     

New aphid species for italy caught by suction trap

Six aphid species new for Italy were caught by suction trap in the Friuli-Venezia Giulia region, Italy. They are:Chaetosiphon chaetosiphon (Nevsky),Illinoia morrisoni (Swain),Myzodium modestum (Hottes),Pseudacaudella rubida (Börner),Schizaphis longicaudata Hille Ris Lambers andTrichosiphonaphis (Xenomyzus)polygonifoliae (Shinji). All species are provided here with characteristic morphological information and line drawings.     

Genetic diversity in landrace populations of Hordeum vulgare L. from Sardinia, Italy, as revealed by RAPDs, isozymes and morphophenological traits

Barley is the second most widely cultivated cereal on the island of Sardinia. It is grown for green fodder, grain and straw, all of which are used for animal feet. The cultivation of modem varieties is limited and many farmers grow local populations of the six-row landrace ‘S'orgiu sardu’. Twelve local populations (20 strains per population) were evaluated for 13 random amplified polymorphic DNA (RAPD) markers, sisisozyme loci and five morphophenological traits. Results showed that Sardinian barley is characterized by a lax six-row spike and is. in general, a spring type. Isozyme polymorphism was delected for only two esterase loci (Est and Est 4). The genetic variation for all types of murkers, was found located mostly within population (about 84%, for isozymes aud morphaphenological traits and 89%, for RAPDs). This finding appears to confirm the hypothesis that the populations studied belong to the same land race called ‘S'orgiu sardu’ by farmers. A geographical differential ion between southern and northern populations was foand in relation to distinct environmental characteristics. Taking into account all types of marker 209 (90.5%) different genotypes were identified out of 231 individuals. The overall results confirm the high variability that can be found within landrace populations, underlining the value of landraccs as a source of genetic variation.     

Rearing equipment on the quality aspects and growth performance of Crassostrea gigas (Thunberg, 1793): Implications for marketability

Shell characteristics and consequent marketability of the oyster Crassostrea gigas are influenced by several factors, and most important of which are the rearing methods and gears. In this study, we investigated the effects of two different gears on the quality aspect (shell shape, weight meat percentage, backward shell shape, epibionts and blisters) and growth rate (weight and length) of C. gigas by adopting the innovative Ortac Oyster Farming System, during the field grow‐out phase of production. Aspect and growth of oysters farmed in Ortac and floating bags were compared. After 14 weeks, the aspect of the animals was influenced by the gear, resulting in a lower quality in the Ortac (i.e. worse shell shape, lower weight meat percentage, higher percentage of backwards and blisters) than in the floating bag, while the incidence of epibionts was less in the Ortac. Regarding the growth performance, total wet weight gained in the Ortac was lower (~28.3 g) than in the floating bag (~32.4 g; p < .05). These results highlight the efficiency of the floating bag as oyster rearing system in the Mediterranean basin and suggest the need to alter the Ortac gear to these environmental conditions.     

Evaluation of estimated weather data for calculating Penman-Monteith reference crop evapotranspiration

Utilizing the weather generator ClimGen, daily solar radiation (R_s) and vapor pressure deficit (VPD) were estimated from temperature data and used to calculate evapotranspiration at five locations, representing tropical, temperate, semi-arid, and arid climates. ClimGen was calibrated for each location using the most recent 2 or 5 years of complete daily weather records. Actual and estimated values were compared on a daily and weekly (7-day running average) basis. Error indices were defined to indicate excellent to poor performance of the estimation methods. Overall in all locations, the ClimGen estimates for both daily R_s and VPD were poor to acceptable. The weekly analyses showed significant improvement in performance for both R_s and VPD estimations in arid and semi-arid locations. Daily reference crop evapotranspiration values using the FAO Penman-Monteith equation (PM ET_o) were calculated using complete daily weather records. These values were compared with (1) ET_o calculated with the PM model, actual temperature data, and ClimGen estimates of daily R_s, VPD, and generated wind speed (PM_Est ET_o), and (2) ET_o calculated solely from actual daily temperature data using a calibrated version of the Hargreaves method (HG_Adj ET_o). The daily PM_Est ET_o results were poor to acceptable in all locations, but analyses for weekly periods showed improved performance to acceptable and good levels for arid and semi-arid locations. The performance of the HG_Adj ET_o method was also poor to acceptable for daily ET estimates in all locations, while weekly analyses showed improvement. A non-calibrated version of the Hargreaves method did not work for either daily or weekly periods. The PM_Est ET_o and HG_adj ET_o methods appeared suitable for weekly periods in arid and semi-arid locations provided that at least 2 years of complete weather records were available to calibrate the parameters required. There was no advantage in using 5 years of weather records for calibration.Communicated by E. Fereres     

Mechanized thinning of walnut plantations established on ex-arable land

The study compares two product strategies—chips versus firewood—and two technological levels—manual versus mechanized—as applied to the thinning of walnut agroforestry plantations, established on ex-arable land. Such plantations are widespread all across Europe, and their establishment was subsidized under the provisions of EU Directive 2080/90, and of regional grant schemes. Field test data were used to build a simple deterministic model for estimating thinning productivity and cost under varying work and economic conditions. This model can assist prospective users when checking the profitability of an operation, or when assessing the competitiveness of alternative options. Removing nurse alder from young walnut plantations yields between 25 and 50 t of fresh biomass per hectare and is crucial to the good development of the stand. The study shows that the removal of nurse alder from walnut plantations is economically viable, and it can also offer some profits if stand and market conditions are favourable. As a rule, the average DBH of removal trees should not be smaller than 12 cm. Best results are obtained with mechanized harvesting, which does not seem to cause heavier stand and soil damage than manual harvesting. Manual harvesting is preferable only if the annual utilization of machinery is very low, and in this case it should be geared to firewood production. The manual whole-tree harvesting (WTH) method used in this study offers the lowest performance under all conditions, and should be replaced with some other manual alternative to WTH. Mechanized WTH offers a significant cost reduction over mechanized short-wood (SWS) harvesting, but this difference is still rather limited: hence, other parameters come into play when deciding what system to apply, and namely product price and mass output. In this respect, one also has to consider the cost of managing the harvesting residue, and that of fertilizing, if soil nutrient depletion is to be feared.