Clinical findings and progression of 10 cases of equine ulcerative keratomycosis (2004–2007)

Ulcerative keratomycosis is a serious sight‐threatening disease of horses and the veterinary literature is replete with cases of poor visual outcome following this condition. During a 3 year period, 10 horses were treated for confirmed keratomycosis at the Veterinary Teaching Hospital (VTH) of the University of Cordoba (Spain). Ulcerative keratomycosis accounted for an average of 8.62% of the total equine ophthalmic admissions during this time and an average of 33.3% of horses were diagnosed with infectious keratitis. Fungi were diagnosed using cytology (n = 4) and/or culture (n = 8) and histopathology (n = 1). Aspergillus sp. was the most commonly isolated fungus. Medical therapy alone or combined medical and surgical treatment was utilised for therapy depending on the clinical condition. Miconazole 1% was the most common topical antifungal therapy employed. Median duration of treatment was 73.12 days. Records were evaluated to determine visual outcome and globe survival.     

Apoptotic and free radical scavenging properties of the methanolic extract of Gentianella alborosea

Gentianella alborosea ("Hercampure") is a Peruvian species used in folk medicine for the treatment of a variety of health disorders. We tested the free radical scavenging (DPPH) and induction of apoptosis on a human uterus tumor cell line (HeLa) by its methanolic extract. The results showed a noticeable radical scavenging activity and a dose-dependent apoptotic effect.     

The inherent variability of water stress indicators in apple, nectarine and pear orchards, and the validity of a leaf-selection procedure for water potential measurements

The following two topics were examined: (1) The variability in the measurement of leaf water potential (LWP), stem water potential (SWP), maximum daily trunk shrinkage (MDS), and soil water tension (SWT) in apple, nectarine and pear orchards; and (2) The validity of a leaf-selection procedure for SWP measurements in commercial apple orchards. 27 trees were selected in an apple orchard, 27 in a nectarine orchard, and 30 in a pear orchard. The trees were close to each other. The measurements comprised of: midday SWP in apple, nectarine and pear; midday LWP in apple; MDS in apple and nectarine; and SWT in pear. The mean and standard errors (SEs) of each water status indicator in each species were calculated for an increasing sample size. The sample sizes required for stable averages were: SWP – 4, 5, and 8 trees for apple, nectarine and pear, respectively; MDS – 17 and 16 trees for apple and nectarine, respectively; SWT – 21 for pear trees. The relative SEs (i.e. percent of population mean) were 2.4, 6.1 and 10.1% in SWP/LWP, MDS and SWT, respectively. Possible explanations for the differing variability of the various water status indicators are discussed. The results show that smaller samples were sufficient to represent SWP and LWP properly than what was required for MDS and SWT. 9 commercial apple plots were selected and about 25 randomly selected leaves were used for midday SWP measurements in each plot (i.e. experimental sets). About 5 leaves on closely adjacent “representative” trees were selected in each of the commercial plots (i.e. commercial sets) and midday SWP was measured. The average difference in SWP between the experimental and the commercial sets was –0.127 MPa. The choice of closely adjacent trees increased the deviation from the experimental sets. The use of a reasonable sample size (n=7) may enable midday SWP to be measured within ±0.15 MPa in most commercial orchards.     

Glandular structures in the major interlobular and extrapancreatic ducts in the guinea pig

The morphology of the pancreatic duct system did not receive much attention as compared to the microanatomy of the exocrine and endocrine pancreas. The histological peculiarities of the excretory duct system are of major importance especially in laboratory animals like guinea pigs. The paper describes the histological peculiarities of the major interlobular and extrapancreatic ducts in guinea pigs. The pancreatic tissue samples were collected from five guinea pigs. For histological investigation, several pancreatic fragments underwent fixation in 10% buffered formalin and were later processed by the standard paraffin technique. Subsequentially, tissue sections were stained by Goldner's trichrome staining. The mucous substances were assessed by Alcian blue and Periodic acid–Schiff staining methods. The interlobular and main pancreatic ducts in the guinea pig present a simple columnar epithelium surrounded by a thick layer of dense connective tissue. The aforementioned epithelium of the main pancreatic ducts includes principal cells, goblet cells and basal cells. Additionally, the ductal epithelium presents occasional unicellular multiloculated intraepithelial mucous glands and prominent extraepithelial glands. The latter adopts a simple or compound tubular feature. The mucus elaborated by the three glandular types is mostly neutral in goblet cells, predominantly acidic in extraepithelial ductal glands, and a similar amount of acidic and neutral mucin in intraepithelial glands. In conclusion, the epithelium-associated mucous glands in the interlobular and main pancreatic ducts in the guinea pig are restricted not only to goblet cells. A substantial mucous discharge probably with a protecting role against irritative pancreatic juice derives from the main ductal intraepithelial and extraepithelial glands.     

Myenteric ganglionitis and intestinal leiomyositis in a Jack Russell terrier

A 9-year, 6-month old spayed female Jack Russell terrier presented with a 3-week history of intermittent vomiting, diarrhoea and weight loss. Serum biochemistry demonstrated severe panhypoproteinaemia, mild hypocalcaemia and mild hypocholesterolaemia, consistent with protein-losing enteropathy. Full-thickness biopsies obtained from the stomach and different sections of small intestine demonstrated histological features of both myenteric ganglionitis and early intestinal leiomyositis. Complete resolution of clinical signs occurred within 1 week of implementing immunosuppressive therapy. At the time of writing, 9 months following diagnosis, the dog remains in remission.     

An unusual clinical presentation of a dog with gastrinoma

Gastrinoma is a rare malignant neuroendocrine neoplasia that results in autonomous gastrin secretion that stimulates hypersecretion of gastric acid, resulting in severe gastric and proximal small intestinal ulcerations. The principal clinical manifestation of gastrinoma is persistent vomiting. This report describes an uncommon manifestation of pancreatic gastrinoma in a dog.     

Septal tryptophan-5-hydroxylase: divergent response to raphe lesions and parachlorophenylalanine

A soluble form of tryptophan-5-hydroxylase activity was found to be present in areas rich in serotonergic terminals (colliculi, hippocampus, septal area, and remaining telencephalon) as well as in brainstem, an area rich in cell bodies. The enzymatic activity in all brain regions, except the septal area, was inhibited to varying degrees following administration of parachlorophenylalanine. Destruction of the raphe nuclei in the midbrain led to a large and comparable decrease in both serotonin content and tryptophan hydroxylase activity of the hippocampus. In contrast, these lesions did not significantly affect the enzymatic activity of the septal area although the serotonin content was decreased by 72 percent. These findings suggest that the major portion of the tryptophan hydroxylase activity of the septal area is uniquely different from that found in other telencephalic areas in that it is not localized in serotonergic nerve terminals nor is it inhibited by parachlorophenylalanine.     

COMPARISON BETWEEN SURVEY RADIOGRAPHY,B‐MODE ULTRASONOGRAPHY,CONTRAST‐ENHANCED ULTRASONOGRAPHY AND CONTRAST‐ENHANCED MULTI‐DETECTOR COMPUTED TOMOGRAPHY FINDINGS IN DOGS WITH ACUTE ABDOMINAL SIGNS

Contrast‐enhanced multi‐detector computed tomography (CE‐MDCT) is used routinely in evaluating human patients with acute abdominal symptoms. Contrast‐enhanced ultrasound (CEUS) continues to be in its infancy as it relates to evaluation of the acute abdomen. The purpose of this study was to compare survey radiography, B‐mode ultrasound, CEUS, and CE‐MDCT findings in canine patients presenting with acute abdominal signs; with a focus on the ability to differentiate surgical from non‐surgical conditions. Nineteen dogs were prospectively enrolled. Inclusion required a clinical diagnosis of acute abdominal signs and confirmed surgical or non‐surgical causes for the clinical signs. Agreement for the majority of recorded imaging features was at least moderate. There was poor agreement in the identification of pneumoperitoneum and in the comparison of pancreatic lesion dimensions for B‐mode vs. CEUS. The CT feature of fat stranding was detected in cases including, but not limited to, gastric neoplasia with perforation, pancreatitis, and small intestinal foreign body. Ultrasound underestimated the size and number of specific lesions when compared with CE‐MDCT. Contrast‐enhanced ultrasound was successful in detecting bowel and pancreatic perfusion deficits that CE‐MDCT failed to identify. Accuracy for differentiation of surgical vs. non‐surgical conditions was high for all modalities; 100%, 94%, and 94% for CE‐MDCT, ultrasonography and survey radiography respectively. Findings indicated that CE‐MDCT is an accurate screening test for differentiating surgical from non‐surgical acute abdominal conditions in dogs. Focused CEUS following CE‐MDCT or B‐mode ultrasonography may be beneficial for identifying potentially significant hypoperfused lesions.     

Investigation of hematological indices of female rabbit blood with addition of an antioxidant preparation to the ration

Homeostasis constants of female rabbits of the Soviet Chinchilla breed aged from 2 to 6 months with addition of the antioxidant preparation Eurotiox Plus Dry to the ration were studied under normal ontogenetic conditions. Age-related changes in the cell composition of female rabbits of different age groups, including the quantitative composition and differential white blood cell count, are shown. The data obtained are analyzed in connection with ontogenetic characteristics of metabolism of the animals.