Production and Identification of Clone Sheep Using Bone Marrow Mesenchymal Stem Cells as Donor Cell

In order to obtain the cloned sheep using bone marrow mesenchymal stem cells（BMSCs）as the donor cells,the BMSCs of sheep were chosen and reconstructed embryos were built to transfer.10 published microsatellite markers were chosen,and the DNA samples from clone sheep,donor cells and surrogate ewes were amplified,and the relationship of father-son（RCP）was analyzed using the Quantity One for genotyping.The results showed that the reconstructed embryos were successfully built for electric fusion using sheep BMSCs as nuclear donor,and making nuclear transplantation into enucleated mature oocytes of which the fusion rate was 80.62%.20 surrogate ewe were chosen to be implanted with the reconstructed embryos at morula stage by implant surgery,and 5 lambs were born and only 3 were survived.The genotype of cloned sheep was in line with the dornor cell and the RCP were more than 99.999%.In conclusion,the first clone sheep were obtained successfully by using BMSCs as a nuclear donor in this experiment.     

桃在低温贮藏过程中品质变化研究

为比较不同类型桃在低温条件下的贮藏特性,以水蜜桃‘美香’和蟠桃‘银河’为试材,研究了低温贮藏过程中果皮颜色、果实硬度、可溶性糖和有机酸含量等的变化。结果表明:低温贮藏期间桃果皮颜色的变化在不同品种间表现有较大差异,‘银河’在贮藏过程中果皮颜色略微加深;2个品种果实硬度总体下降比较明显;不同品种果实可溶性糖含量的变化比较复杂,其中果实可溶性糖总量波动较小,蔗糖含量在贮藏后期明显下降;有机酸总量方面,‘美香’和‘银河’总体都呈现前期下降,至贮藏后期小幅上升的变化趋势,其中柠檬酸的含量在贮藏后期出现明显下降。     

Immunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA

Inactivated vaccines of Avibacterium paragallinarum provide protection and reduce the economic losses caused by infectious coryza. However, inactivated bacterins provide protection only against the Page serovars included in the vaccine. In this study, we investigated the immunological properties of a functional recombinant haemagglutinin protein (rHagA) derived from a Taiwan isolate strain A9 as the immunogen for vaccination. The rHagA subunit vaccine protected 71% of immunized chickens against 10(10) colony-forming units (CFU) of viable A9. Vaccinated chickens which showed no clinical signs of coryza developed haemagglutination inhibition (HI) titers of 1:10 or greater. Haemagglutination (HA) of serovars A and C was not affected by the presence of rHagA specific antiserum. The HA of rHagA could only be induced against formaldehyde-fixed chicken red blood cells (FA-RBCs). These results suggested that HagA is a moderate immunogen and might not be a major haemagglutinin in vivo. However, HagA might be involved in haemagglutination when treated serovar C aggregates fixed RBCs in vitro.     

国内外致泻大肠埃希氏菌O157:H7的检测标准比较及建议

致泻大肠埃希氏菌O157:H7（STEC O157:H7）是大肠埃希氏菌中致病性最严重的一种食源性致病菌,主要存在于牛肉、牛奶、水果及其制品中,对身体健康造成很大危害,甚至引发死亡。食品中STEC O157:H7检测尤为重要。本文对国内外STEC O157:H7的检测标准进行比较,提出我国标准在样品前处理、快速筛选方法的应用等方面需要加强,以便为该菌快速准确检测提供帮助,实现与国际标准化体系建设接轨,满足实验室检测需要。     

明胶用量及其与卡拉胶复配对奶冻品质的影响

[目的]奶冻在储存过程中常出现失水现象,为提高奶冻贮藏中的品质和保水性。[方法]以不同时间下奶冻质构特性、持水力、感官品质指标评价奶冻品质,探究明胶添加量以及明胶-卡拉胶复配对奶冻品质的影响。[结果]随明胶添加量增大,水析率、回复性和弹性逐渐减小,硬度和咀嚼度逐渐增大,感官品质先提升后下降,明胶添加量为1.8%时奶冻品质最好;在总胶体用量为1.8%情况下,卡拉胶比例增大,水析率和弹性变化幅度小,硬度先增大后减小再增大,咀嚼度先减小后增大,回复性减小,感官品质先降低后提升最后持续降低,卡拉胶比例为7%时其奶冻品质最好。[结论]1.8%的胶体添加量下添加7%的卡拉胶与明胶复配提高了奶冻的贮藏品质,胶体复配可改善奶冻贮藏中品质下降问题。     

A FINITE ELEMENT ALGORITHM FOR PARALLEL PROCESSING OF MULTI-FRONTS

In this paper,a new algorithm for solving large-scale finite element system is presented, which utilizes the architecture of parallel processing. Firstly, the structure is partitioned into sub-regions. Then multi-fronts are used to assemble and eliminate concurrently in every sub-region. Thus the condensed interface stiffness matrices and load vectors are obtained. By serially assembling and solving global equation to get interface displacements and backing to every sub-region, the inter-nodal displacements and element stresses can be solved. The results of computation show that it can not only increase the speed of computation,but also save the memory space of computer effectively. This algorithm is an effective method for solving large-scale structural finite element systems.     

Study on Landscape Planning of Mourtcainous City

:In accordance with the problems, occurred in the mountainous city's construction, especially the crises such as the destruction of ecological and humanistic environment, the loss of traditional culture and characteristics and the disorder of urban structure, this paper discusses the distinctive landscape characteristic of mountainous city thoroughly.     

A Spot Price Model with Var Cost

This paper has applied the var cost to the theory of spot price.An augmented Lagrange method is employed to develop the exact model of spot price of active power and reactive power,and the physical means of Lagrange multipliers are explained.The numerical examples are given to demonstrate the validity of the proposed model.     

Acoustic Evaluation of the Concert Hall Based on Simulated Impulse Responses

The impulse responses and other acoustical parameters of Huangpu Teenager Palace were measured. Meanwhile, the acoustical simulation based on software ODEON was carried out as well. The parameters based on computer simulation are compared with the measured ones. This case study shows that the auralization technique based on computer simulation can be used for predicting the acoustical quality of a hall at its design stage.