Microarray-based miRNAs profiling in lower limb arteriosclerosis of diabetic rats

AIM: To establish the profiling of microRNAs (miRNAs) in the lower extremity arterial tissue between diabetic rats with lower limb arteriosclerosis (DAS) and diabetic rats with normal lower limb (DN), and to explore the possible molecular mechanisms involved in aberrant miRNA expression in DAS. METHODS: The rat models of DAS and DN were successfully established. The respective lower extremity arterial tissue was isolated. The total miRNAs were purified for a hybridization detection by miRNA microarray. The results of chip scanning and data were analyzed and verified by RT-qPCR. RESULTS: Ten miRNAs related to DAS, including rno-miR-206-3p, rno-miR-133a-5p, rno-miR-133b-3p, rno-miR-133a-3p, rno-miR-325-5p, rno-miR-675-3p, rno-miR-411-5p, rno-miR-329-3p, rno-miR-335 and rno-miR-126a-3p, were determined. All 10 abnormally expressed miRNAs were up-regulated. The validating results of RT-qPCR confirmed 9 of the miRNAs in line with chip expression. Just rno-miR-335 showed the opposite between PCR detection and microarray result. CONCLUSION: A group of miRNAs in diabetic rats suffering from lower limb arteriosclerosis plays an important role in the vascular atherosclerosis process. The abnormal expression of miRNAs is likely to affect the vascular atherosclerosis process.     

piggyBac转座子介导的家蚕细胞转基因研究初探

为探讨稳定转化家蚕细胞表达外源基因的新方法,以家蚕核型多角体病毒(BmNPV)极早期蛋白基因(ie-1)启动子元件驱动新霉素抗性基因(neor),并克隆至具有绿色荧光蛋白基因(gfp)标记的昆虫转座子载体piggyBac中,构建转基因载体pigA3-IE-Neo。以该载体转染家蚕BmN细胞,用终浓度800μg/mL的遗传霉素(geneticin,G418)筛选3个月,获得了稳定转化的细胞,呈现绿色荧光的细胞数达80%以上。通过PCR鉴定证实了细胞基因组DNA中neor基因和gfp基因的存在。     

利用家蚕表达合成人表皮生长因子(EGF)及产物对胃粘膜损伤的修复作用

基于家蚕杆状病毒密码子的偏爱性,对人表皮生长因子基因(hEGF)的部分密码子进行修改后合成一个新的基因,命名为CSEGF。所合成的CSEGF能编码同hEGF完全一致的氨基酸序列,并被重组进家蚕杆状病毒获得重组Bm-BacCSEGF。重组病毒感染家蚕后,该基因在家蚕中的表达量达28.4μg/mL幼虫血淋巴及33.07μg/mL蛹血淋巴。将表达CSEGF的蚕蛹经冻干直接制成EGF冻干粉胶囊,经测定冻干粉中EGF的质量比达140μg/g。动物模型试验显示该冻干粉对酸诱导的急性胃粘膜损伤的SD大鼠有显著的修复作用,对大鼠裸鼠的肾细胞生长也有明显的促进作用。     

遥感技术在山西省森林资源清查中的应用

山西省林业部门早在“四五”期间就广泛应用黑白航片,全野外调绘,进行全省森林资源清查。完成了林业部下达的任务。“六五”期间山西省农业遥感应用科学研究所,应用陆地卫星影像,进行全省森林资源解译,获得了全省森林分布情况;“七五”期间遥感所与北京林科院协作,应用陆地卫星“ＴＭ·ＣＣＴ”磁带,经计算机专题提取信息,对关帝山林区森林进行了分类,求得了各类树种的分布与面积,同时还完成了“三北”防护林建设工程的试点县——大宁县森林资源动态遥感调查,并应用彩红外航片结合地面样方进行了沙棘资源的调查,为我省森林资源合理开发利用,提供了科学依据     

葡萄糖氧化酶基因在棉花中的高效转化——转化与抗性愈伤组织的诱导和筛选

试验探讨了利用农杆菌介导法把葡萄糖氧化酶基因（ＧＯ基因）转化到棉花中影响转化和抗性愈伤组织诱导的一些因素。结果表明,５～７ｄ 苗龄的无菌苗是做转化的最佳时期,以３Ｄ＋ ２Ｌ处理的无菌苗效果好。下胚轴的形态学部位对转化的影响差异不大。菌株的活力状态是影响转化出愈率的主要因素之一。以菌株的培养时间为依据简便有效。试验还发现,培养基的固化程度不同,出愈率有很大差异,固化程度高,异常愈伤组织增多,正常愈伤组织的出愈率降低。这可能与其活性氧平衡被打破对细胞造成伤害有关。对坑性愈伤组织的筛选次数也做了讨论。     

A Method for Solving Strong Nonlinear Vibration

A method,stroboscopic harmonicbalance method which is used to solve strong nonlinear system, is proposed. The method is used to find the solution of strong nonlinear system. In investigation,the existence conditions of primary resonance solution and subharmonic resonance solutions are obtained. The results which are obtained by the stroboscopic harmonicbalance method are compared with those by computer numerical simulation. The results show that the stroboscopic harmonicbalance method is correct in qualitative analysis and it can meet the demands in engineering.     

溧阳鸡产肉性能及肌肉品质的测定

以8周龄溧阳鸡为素材,测定其屠宰性能、肉品质和化学指标。结果表明:溧阳鸡产肉性能良好,8周龄屠宰率在88%以上,全净膛率在60%以上;胸、腿肌pH值分别为5.93、6.40;胸肌失水率大于腿肌,但胸肌剪切力小于腿肌;腿、胸肌水分含量为710-740g·kg-1;脂肪含量母鸡胸、腿肌分别为8.4、23.0g·kg-1,公鸡分别为6.6、20.6g·kg-1;蛋白质含量母鸡胸、腿肌分别为218.4、181.0g·kg-1,公鸡分别为228.1、194.7g·kg-1。各指标性别间差异均不显著。     

我国主要类型土壤的有效态硒及其与某些土壤性质的关系

Forty-eight soil profiles were sampled from different ecological and pedogeochemical areas of China and their available selenium (Av-Se) contents were determined. Results showed that the content of Av-Se in the profiles of soils in China ranged within 2.49~18.10 μg kg^-1, with a mean value of 10.01 μg kg^-1, and was in the sequence of Ferralisols > Luvisols > Isohumisols > Aridisols. The correlation analysis between Av-Se and the soil physical-chemical properties revealed that the correlations between Av-Se and O.M., CEC, Fe₂O₃, Al₂O₃, ignition loss, Co and Zn were positive at a highly significant level, but those between Av-Se and pH, base saturation, CaO, Ba and Sr were all negative at a highly significant level. The reason of some diseases related to the Se deficiency might be the leaching loss of selenium in some soils.     

气候智慧型农业措施对中国农田土壤有机碳含量影响的Meta分析

气候智慧型农业（Climate-smart agriculture,CSA）作为一种致力于实现提高农业效率、增强农业适应性和减少温室气体排放三大目标的新型农业,已经在全球农业区得到了广泛实施。本研究旨在探究4种CSA措施——生物炭、免耕、绿肥和秸秆还田对我国农田土壤有机碳（Soil organic carbon,SOC）含量的影响,以期为我国农田SOC管理和CSA的推广提供科学依据。数据来源于CNKI和Web of Science两大文献数据库,共收集了2002—2022年78篇相关的大田试验文献,并摘录470条试验结果和试验背景信息,利用整合分析（Meta-analysis）的方法量化4种CSA措施对我国农田SOC含量的作用效果及作用过程中的外部影响因素。结果表明：相较于传统农业,CSA措施对农田SOC含量具有显著的提升作用,总体可提高18.19%。4种措施的实施效果表现为生物炭>秸秆还田>绿肥>免耕,SOC含量增长率分别为46.67%、17.08%、9.32%、7.34%。区域环境因素（气温、降水、土壤深度、土壤pH、初始SOC含量等）和管理方式（试验持续时长、试...     

中国人为土的多样性

Human activities make strong effects on soil formation. Anthropogenic soils are much more intensive and extensive in China for their history of agricultural production can be dated back to more than 7 000 years ago. Owing to different physical conditions and land uses, the anthropogenic soil-forming processes are various. Anthrosols are proposed, and the corresponding soil order is set up in Chinese Soil Taxonomy (CST). Mainly based on 6 Anthropogenic diagnostic horizons, which are anthraquic epipedon, hydragric horizon, irragric epipedon, cumulic epipedon, fimic epipedon and agric horizon, the Anthrosols Order is subdivided into 2 soil suborders and 4 soil groups. Meanwhile the classification of Anthrosols in CST has been basically accepted as the classification of Anthrosols in World Reference Base for Soil Resources (WRB).